Reticulated platelet monitoring after autologous peripheral haematopoietic progenitor cell transplantation

Martinelli, G.; Merlo, Patrizia; Fantasia, R; Gioia, Francesco; Crovetti, G

doi:10.1016/j.transci.2009.03.018

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…Previous studies used different definitions for platelet recovery, for example, platelet count ≥30 × 10 9 /L for three consecutive days, seven consecutive days with a platelet count ≥20 × 10 9 /L without platelet transfusions, or a spontaneous increase in PLT count without platelet transfusions . Despite the fact that we used RCV to define platelet recovery, the interval between increase in immature platelets and platelet recovery we found appears similar, that is, approximately 2 d .…”

Section: Discussionmentioning

confidence: 71%

“…Several more recent publications confirmed this observation (2,(10)(11)(12). This might also be of interest in patients undergoing stem cell transplantation (SCT) for hematological malignancies (7,(13)(14)(15)(16)(17)(18)(19). In the post-transplant period, patients have a significantly lower concentration of platelets due to a decrease in thrombopoiesis.…”

mentioning

confidence: 73%

“…If a reliable prediction of the natural recovery of the platelet number within a few days is feasible, a valid decision whether or not to give a prophylactic platelet transfusion would be facilitated. The number of immature platelets could be used as such a predictor, as various studies have described a rise in the number of immature platelets shortly preceding platelet recovery . Some studies also proposed a cutoff value for immature platelets predicting platelet recovery .…”

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confidence: 99%

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Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation

Linden

Klinkenberg

Meex

et al. 2014

European J of Haematology

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ObjectivesA period of thrombocytopenia is common after stem cell transplantation (SCT). To prevent serious bleeding complications, prophylactic platelet transfusions are administered. Previous studies have shown that a rise in immature platelets precedes recovery of platelet count. Our aim was to define a cutoff value for immature platelets predicting thrombopoietic recovery within 2 d.MethodsHematological parameters were measured on the Sysmex XN hemocytometer. We calculated reference change values (RCV) for platelets in eight healthy individuals as marker for platelet recovery. To define a cutoff value, we performed ROC analysis using data from 16 autologous SCT patients.ResultsRCV for platelet concentration was 14.1%. Platelet recovery was observed 13 (median; range 9–31) days after SCT. Increase in immature platelet fraction (IPF) before platelet recovery was seen in all autologous SCT patients. Optimal cutoff IPF was found to be 5.3% for platelet recovery within 2 d (specificity 0.98, sensitivity 0.47, positive predictive value 0.93).ConclusionsWe identified an optimal cutoff value for IPF 5.3% to predict platelet recovery after autologous SCT within 2 d. Implementing this cutoff value in transfusion strategy may reduce the number of prophylactic platelet transfusions.

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Section: Discussionmentioning

confidence: 71%

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confidence: 73%

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confidence: 99%

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Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation

Linden

Klinkenberg

Meex

et al. 2014

European J of Haematology

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“…It is known that activationdependent mechanisms enhance platelet turnover and contribute to the presence of RPs in circulation. Therefore, assessment of RP could differentiate peripheral destruction vs suppression of bone marrow thrombopoiesis [12].…”

Section: Contents Lists Available At Sciencedirectmentioning

confidence: 99%

Clinical applicability of reticulated platelets

Dusse

Freitas

2015

Clinica Chimica Acta

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“…As an alternative to platelets isolated by platelet apheresis or concentration from whole blood donations used for therapeutic platelet substitution [1][2][3], in vitro generation of platelets or megakaryocytically committed progenitors from hematopoietic stem and progenitors cells (HPCs) in liquid cultures has been proposed [4,5]. Cytokines which have been investigated for this purpose include thrombopoietin (TPO), stem cell factor (SCF), interleukins (IL-3, IL-11) and fetal liver tyrosine kinase 3 ligand (FLT3L) [6,7].…”

Section: Introductionmentioning

confidence: 99%

Platelet Precursor Cells Can Be Generated from Cultured Human CD34+ Progenitor Cells But Display Recirculation into Hematopoietic Tissue upon Transfusion in Mice

Hintze¹,

Rüster²,

Seifried³

et al. 2010

Transfus Med Hemother

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Background: Whereas ex vivo expanded megakaryocytic progenitor cells have been investigated for their ability to support platelet regeneration, the question whether more mature platelet-like particles expanded from hematopoietic progenitor cells may be useful for transfusion purposes remains largely elusive. Methods: Human peripheral blood progenitor cells (PBPCs) were enriched using surface expression of CD34 by immunoselection. CD34+ enriched PBPCs were expanded ex vivo in serum-free medium supplemented with cytokines. As a proof-of-principle, distribution of expanded CD61+ particles was analyzed after transfusion into Non-Obese Diabetic/ Severe Combined Immunodeficiency (NOD/SCID) mice. Results: Highest ex vivo expansion for CD41+/CD61+ cells was achieved when medium was supplemented with SCF, TPO and IL-3. During expansion culture, CD34 marker expression decreased from 85 to 2–8%, while megakaryocytic cells appeared and CD41 and CD61 expression increased from 3 to about 30%. After transfusion of the expanded cells in NOD/SCID mice, CD61+ cells located mainly to bone marrow and to a lesser degree to spleen, but also circulated in blood. Conclusion: Platelet-like particles using cytokine-substituted serumfree medium can be generated efficiently from CD34+ expansion cultures, but mainly home to hematopoietic tissue.

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Reticulated platelet monitoring after autologous peripheral haematopoietic progenitor cell transplantation

Cited by 9 publications

References 21 publications

Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation

Immature platelet fraction measured on the Sysmex XN hemocytometer predicts thrombopoietic recovery after autologous stem cell transplantation

Clinical applicability of reticulated platelets

Platelet Precursor Cells Can Be Generated from Cultured Human CD34+ Progenitor Cells But Display Recirculation into Hematopoietic Tissue upon Transfusion in Mice

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