Reticulated Platelet Counts in the Diagnosis of Acute Immune Thrombocytopenic Purpura

Saxon, Ben; Blanchette, Victor S.; Butchart, Sheila; Lim-yin, Jim; Poon, Annette

doi:10.1097/00043426-199801000-00007

Cited by 50 publications

(34 citation statements)

References 20 publications

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“…Richards & Baglin (1995) studied 79 thrombocytopenic patients (25 with immune thrombocytopenia and a normal bone marrow, 31 with bone marrow aplasia, and seven with consumptive coagulopathy) and found that the patients with increased peripheral platelet destruction had a higher RP% than those with decreased platelet production. Similar results have been reported by other groups (Watanabe et al, 1995;Chavda et al, 1996;Peterec et al, 1996;Koike et al, 1998;Saxon et al, 1998;Stohlawetz et al, 1999). It has even been postulated that the RP% alone has an even better predictive value than PA-IgG measured with flow cytometry for the diagnosis of autoimmune thrombocytopenia .…”

Section: Rp In Healthy Controls and Patientssupporting

confidence: 87%

“…Unstained control samples have also been used in setting the gate of positivity (Romp et al, 1994;Richards & Baglin, 1995;Tàssies et al, 1995). Recently, lyophilised platelets were also introduced as control samples (Saxon et al, 1998). However, there is no internationally agreed system of standardisation or consensus for acquisition of data or for their analysis.…”

Section: Measurement Of Rpmentioning

confidence: 99%

“…As controls, either lyophilised platelets, erythrocyte fluorescence, unstained samples, control cut-off values or RNAase treatment have been used (Ault et al, 1992;Bonan et al, 1993;Rinder et al, 1993;Romp et al, 1994;Richards & Baglin 1995;Saxon et al, 1998). Here, RNAase was not applied in every test series, rather an internal control sample was used.…”

Section: Measurement Of Reticulated Platelets In Thrombocytopeniamentioning

confidence: 99%

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Autoimmune Thrombocytopenia

2017

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Section: Rp In Healthy Controls and Patientssupporting

confidence: 87%

Section: Measurement Of Rpmentioning

confidence: 99%

Section: Measurement Of Reticulated Platelets In Thrombocytopeniamentioning

confidence: 99%

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Autoimmune Thrombocytopenia

2017

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“…Kienast and Schmitz initiated a breakthrough in the field when they described a flow cytometric technique for analyzing retPLT, based on RNA staining by thiazole orange [15]. In subsequent years, several research groups published their findings in a wide variety of conditions like thrombocytopenia [16][17][18][19][20][21], thrombocytosis [22,23], after stem cell transplantation [24][25][26][27], hereditary platelet diseases [28,29], thrombo-embolic disorders [30,31], kidney disease [32][33][34], preeclampsia [35], hyperthyroidism [36] and in healthy and thrombocytopenic neonates [37,38]. The overall conclusion from these studies is that retPLT in blood represent a useful non-invasive marker of megakaryopoietic activity in the bone marrow.…”

Section: Reticulated Platelet Methods -Flow Cytometrymentioning

confidence: 99%

Reticulated platelets: analytical aspects and clinical utility

Hoffmann

2014

Clinical Chemistry and Laboratory Medicine (CCLM)

136

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Reticulated platelets are immature platelets circulating in blood; they reflect the activity of megakaryopoiesis in the bone marrow. Therefore, they can be used as a non-invasive test in patients with thrombocytopenia in various clinical conditions. The preferred method of analysis is by flow cytometry. However, there is an evident lack of analytical standardization, making it difficult to compare results obtained in different laboratories. Currently, two types of hematology analyzers are on the market offering fully automated measurement of reticulated or immature platelets: the high end analyzers manufactured by Sysmex (XE-and XN-series) and Abbott (CELL-DYN Sapphire). Although the methods are essentially different and cannot be used interchangeably, both have been proven to have clinical utility. Reticulated or immature platelet assays are useful for the differential diagnosis of thrombocytopenia and for monitoring bone marrow recovery after chemotherapy or stem cell transplantation. These assays may aid clinicians in platelet transfusion decisions when recovery from thrombocytopenia is imminent. In addition, preliminary findings indicate that there is a rationale for reticulated or immature platelets for risk stratification in acute coronary syndromes and for monitoring the effect of treatment with antiplatelet drugs in patients with coronary artery diseases. The aim of this paper is to present the present technology available for measuring reticulated platelets as well as an overview of the current status of clinical application. This overview also indicates that more research is needed before reticulated or immature platelet assays can be applied in other clinical conditions than thrombocytopenia and after transplantation.

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“…4 It has been suggested that this can provide a reliable indication of the rate of thrombopoiesis, similar to the use of the erythrocyte reticulocyte count to evaluate erythropoiesis. 4,5 Several studies have demonstrated the usefulness of evaluation of reticulated platelets to characterize thrombocytopenia [6][7][8][9][10][11][12][13] or thrombocytosis. 14 Glycocalicin (GC), which is a carbohydrate-rich hydrophilic fragment that can be rapidly cleaved from the extramembranous portion of the ␣-chain of glycoprotein Ib by various proteases, 15,16 has been proposed as a novel platelet specific marker for platelet membrane injury in vivo.…”

mentioning

confidence: 99%

Characterization of autotransplant-related thrombocytopenia by evaluation of glycocalicin and reticulated platelets

Catani

Vianelli

Luatti

et al. 1999

Bone Marrow Transplant

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Summary:Thrombocytopoiesis of 21 multiple myeloma patients undergoing single or double transplant regimen was characterized by measuring the level of reticulated platelets and plasma glycocalicin. Since reticulated platelets are an index of thrombopoietic activity and glycocalicin plasma values are related to platelet damage and turnover, it may be possible to perform a novel type of analysis of the thrombopoietic compartment during the mobilizing regimen and during transplantrelated chemotherapy. Patients underwent mobilizing therapy and first transplant. Some randomized patients also underwent a second transplant with mobilized peripheral blood stem cells. The results show that the percentage of reticulated platelets decreased after therapy and then gradually increased in the recovery phase either during first or second transplant. By contrast, the percentage of reticulated platelets increased until day +8 and then gradually decreased during the mobilizing regimen. The glycocalicin index (glycocalicin plasma value normalized for the individual platelet count) increased significantly both during the course of mobilization and after transplant-related chemotherapy when the platelet number was at its nadir. However, the glycocalicin index was more elevated after transplantrelated chemotherapy than after the mobilizing regimen. Our findings suggest that chemotherapy-related thrombocytopenia may be due to a dual mechanism: thrombocytopenia results from decreased platelet production in addition to increased platelet damage and possible destruction.

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Reticulated Platelet Counts in the Diagnosis of Acute Immune Thrombocytopenic Purpura

Cited by 50 publications

References 20 publications

Autoimmune Thrombocytopenia

Autoimmune Thrombocytopenia

Reticulated platelets: analytical aspects and clinical utility

Characterization of autotransplant-related thrombocytopenia by evaluation of glycocalicin and reticulated platelets

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