Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Lock, John G.; Jones, Max; Askari, Janet A.; Gong, Xiaoyi; Oddone, Anna; Olofsson, Helene; Göransson, Sara; Lakadamyali, Melike; Humphries, Martin J.; Strömblad, Staffan

doi:10.1038/s41556-018-0220-2

Cited by 113 publications

(168 citation statements)

References 66 publications

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“…This likely frees up a pool of actin monomers (Kaur et al, 2014), which is then used to assemble a thin (Clark et al, 2013), mechanically rigid (Fischer-Friedrich et al, 2015), cortical actomyosin network that drives mitotic rounding (Reinsch & Karsenti, 1994;Ragkousi & Gibson, 2014;Sorce et al, 2015). While the mechanisms underlying this mitotic switch in actin organisation are not well understood, the process likely involves the following: (i) the loss of interphase focal adhesions (Dix et al, 2018;Lock et al, 2018), (ii) the loss of Arp2/3-dependent lamellipodia (Ibarra et al, 2005;Bovellan et al, 2014;Rosa et al, 2015) and (iii) the activation of formins downstream of Ect2/Pbl and the GTPase Rho (Maddox & Burridge, 2003;Matthews et al, 2012;Rosa et al, 2015;Chugh et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis

et al. 2019

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Cells going through mitosis undergo precisely timed changes in cell shape and organisation, which serve to ensure the fair partitioning of cellular components into the two daughter cells. These structural changes are driven by changes in actin filament and microtubule dynamics and organisation. While most evidence suggests that the two cytoskeletal systems are remodelled in parallel during mitosis, recent work in interphase cells has implicated the centrosome in both microtubule and actin nucleation, suggesting the potential for regulatory crosstalk between the two systems. Here, by using both in vitro and in vivo assays to study centrosomal actin nucleation as cells pass through mitosis, we show that mitotic exit is accompanied by a burst in cytoplasmic actin filament formation that depends on WASH and the Arp2/3 complex. This leads to the accumulation of actin around centrosomes as cells enter anaphase and to a corresponding reduction in the density of centrosomal microtubules. Taken together, these data suggest that the mitotic regulation of centrosomal WASH and the Arp2/3 complex controls local actin nucleation, which may function to tune the levels of centrosomal microtubules during passage through mitosis.

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Section: Introductionmentioning

confidence: 99%

Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis

et al. 2019

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“…In line with the role of PCDH7 during adhesion-dependent cytokinesis, we observed a significant cytokinesis failure in PCDH7 KO RPE1 cells when myosin activity was abolished by blebbistatin treatment (Figure 6). The remodeling of mitotic adhesion assists dividing cells for separation and re-spreading (Dix et al, 2018;Lock et al, 2018). Integrin dependent cell-substrate adhesion has been shown to be the dominant player of cytokinesis in adherent non transformed human cells (Dix et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

ZDHHC5 targets Protocadherin 7 to the cell surface by a palmitoylation-dependent mechanism to promote successful cytokinesis

Küçük

Yigit

Degirmenci

et al. 2020

Preprint

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Successful cell division requires dramatic reorganization of the cell cortex in coordination with actomyosin cytoskeleton organization, membrane trafficking and cell adhesion. Although the contractile actomyosin ring is considered as hallmark of cytokinesis, in some cell types cell adhesion systems have been shown to drive cytokinesis independently from actomyosin function. We previously reported that Protocadherin 7 (PCDH7) localizes to the mitotic cortex which is required for building up the full mitotic rounding pressure. Here, we show that PCDH7 localizes to the mitotic cell cortex and to the cleavage furrow by a palmitoylation-dependent mechanism. At the cleavage furrow, PCDH7 facilitates the activation of myosin II and successful cytokinesis. Strikingly, PCDH7 promotes cytokinesis even when the myosin II contractility and integrin mediated adhesion are blocked. This work describes a palmitoylation-dependent cortical reorganization which promotes cytokinesis under different conditions.

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“…On the other side, intra-and extracellular parameters can affect the ability of αVβ3 integrin to select between different ligands. Interestingly, αVβ5 integrin was recently shown to contribute to non-canonical integrin functions either in so called reticular adhesions or by associating with clathrin containing structures (Zuidema et al, 2018, Lock et al, 2018, Baschieri et al, 2018. The switch between these structures and focal adhesions is regulated by different intra-and extracellular parameters including actomyosin-contractility.…”

Section: The Vitronectin Receptor and The Cellmentioning

confidence: 99%

Ligand binding promiscuity of αVβ3 integrin is enlarged in response to mechanical force

Bachmann

Schäfer

et al. 2017

Preprint

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αVβ3 integrin recognizes multiple extracellular matrix proteins, including vitronectin (Vn) and fibronectin (Fn). However, cell experiments are frequently performed on homogenously coated substrates with only one integrin ligand present. Here, we employed binary-choice substrates of Fn and Vn to dissect αVβ3 integrin-mediated binding to both ligands on the subcellular scale. Superresolution imaging revealed that αVβ3 integrin preferred binding to Vn under various conditions. In contrast, binding to Fn required mechanical load on αVβ3 integrin. Integrin mutations, structural analysis, and molecular dynamics simulations established a model where the extended-closed conformation of αVβ3 integrin binds Vn but not Fn. Force-mediated hybrid domain swing-out characterizes the extended-open conformation needed for efficient Fn binding. Thus, force-dependent conformational changes in αVβ3 integrin increase the number of available ligands and therefore the ligand promiscuity of this integrin. These findings for αVβ3 integrin were shown to regulate cell migration and mechanotransduction differentially on Fn compared to Vn and therefore to regulate cell behavior.

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Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis

Abstract: Reticular adhesions are a distinct class of cell-matrix adhesions that mediate attachment during mitosis.

Cited by 113 publications

References 66 publications

Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis

Local actin nucleation tunes centrosomal microtubule nucleation during passage through mitosis

ZDHHC5 targets Protocadherin 7 to the cell surface by a palmitoylation-dependent mechanism to promote successful cytokinesis

Ligand binding promiscuity of αVβ3 integrin is enlarged in response to mechanical force

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