Retention of D genome chromosomes in pentaploid wheat crosses

Martin, Anke; Simpfendorfer, S.; Hare, Ray A.; Eberhard, F.; Sutherland, Mark W.

doi:10.1038/hdy.2011.17

Cited by 18 publications

(19 citation statements)

References 16 publications

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“…The present study was designed according to this rational, along with additional considerations, to construct four pentaploid wheat lines with distinct features that are suitable to specifically address the question, i.e., whether the capacity to buffer and sustain imbalanced D-genome chromosomes by the BBAA component of hexaploid wheat is an evolved trait. Our results, based on high resolution FISH/GISH karyotyping of large numbers of immediate progeny cohorts of each of the four pentaploid wheat lines, have confirmed the previously only implied possibility ( Martin et al, 2011 ; Padmanaban et al, 2017a ). Our results also provide additional insights into the extent and trend of numerical and structural chromosome instabilities in the pentaploid wheat-derived progenies.…”

Section: Introductionsupporting

confidence: 85%

“…Recent years have witnessed a renewed interest in the generation of pentaploid wheat for the purpose of reciprocally introgressing desirable traits from one species to the other (reviewed in Padmanaban et al, 2017b , 2018 ). Apart from the practical success ( Wang et al, 2005 ; Eberhard et al, 2010 ; Padmanaban et al, 2017a , b ), an interesting observation is that, in the progenies of pentaploid wheat, there exists a significant positive correlation between proportions of the hexaploid wheat BBAA genomic content and the retention of unbalanced D chromosome ( Martin et al, 2011 ; Padmanaban et al, 2017a ). This finding suggests that the BBAA components of hexaploid wheat have a stronger capacity to retain unbalanced D chromosomes than that of the tetraploid wheat BBAA genome.…”

Section: Introductionmentioning

confidence: 99%

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The Capacity to Buffer and Sustain Imbalanced D-Subgenome Chromosomes by the BBAA Component of Hexaploid Wheat Is an Evolved Dominant Trait

Deng

Sha

et al. 2018

Front. Plant Sci.

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Successful generation of pentaploid wheat (genome, BBAAD) via interspecific hybridization between tetraploid wheat (BBAA) and hexaploid wheat (BBAADD) holds great promise to mutually exchange desirable traits between the two cultivated wheat species, as well as providing a novel facet for evolutionary studies of polyploid wheat. Taking advantage of the viable and fertile nature of an extracted tetraploid wheat (ETW) with a BBAA genome that is virtually identical with the BBAA component of a hexaploid common wheat, and a synthetic hexaploid wheat, we constructed four pentaploid wheats with several distinct yet complementary features, of which harboring homozygous BBAA subgenomes is a common feature. By using a combined FISH/GISH method that enables diagnosing all individual wheat chromosomes, we precisely karyotyped a larger number of cohorts from the immediate progenies of each of the four pentaploid wheats. We found that the BBAA component of hexaploid common wheat possesses a significantly stronger capacity to buffer and sustain imbalanced D genome chromosomes and appears to harbor more structural chromosome variations than the BBAA genome of tetraploid wheat. We also document that this stronger capacity of the hexaploid BBAA subgenomes behaves as a genetically controlled dominant trait. Our findings bear implications to the known greater than expected level of genetic diversity in, and the remarkable adaptability of, hexaploid common wheat as a staple crop of global significance, as well as in using pentaploidy as intermediates for reciprocal introgression of useful traits between tetraploid and hexaploid wheat cultivars.

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Section: Introductionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

The Capacity to Buffer and Sustain Imbalanced D-Subgenome Chromosomes by the BBAA Component of Hexaploid Wheat Is an Evolved Dominant Trait

Deng

Sha

et al. 2018

Front. Plant Sci.

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“…Variation is expected in the transmission of D-genome chromosomes to subsequent generations [81,82]. It also means that it will not be possible to replicate the phenotypic data recorded on the DGRH 1 lines, unless the segregating lines are confirmed for the presence of a particular chromosome with the phenotypes.…”

Section: Discussionmentioning

confidence: 99%

Physical mapping resources for large plant genomes: radiation hybrids for wheat D-genome progenitor Aegilops tauschii

et al. 2012

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BackgroundDevelopment of a high quality reference sequence is a daunting task in crops like wheat with large (~17Gb), highly repetitive (>80%) and polyploid genome. To achieve complete sequence assembly of such genomes, development of a high quality physical map is a necessary first step. However, due to the lack of recombination in certain regions of the chromosomes, genetic mapping, which uses recombination frequency to map marker loci, alone is not sufficient to develop high quality marker scaffolds for a sequence ready physical map. Radiation hybrid (RH) mapping, which uses radiation induced chromosomal breaks, has proven to be a successful approach for developing marker scaffolds for sequence assembly in animal systems. Here, the development and characterization of a RH panel for the mapping of D-genome of wheat progenitor Aegilops tauschii is reported.ResultsRadiation dosages of 350 and 450 Gy were optimized for seed irradiation of a synthetic hexaploid (AABBDD) wheat with the D-genome of Ae. tauschii accession AL8/78. The surviving plants after irradiation were crossed to durum wheat (AABB), to produce pentaploid RH1s (AABBD), which allows the simultaneous mapping of the whole D-genome. A panel of 1,510 RH1 plants was obtained, of which 592 plants were generated from the mature RH1 seeds, and 918 plants were rescued through embryo culture due to poor germination (<3%) of mature RH1 seeds. This panel showed a homogenous marker loss (2.1%) after screening with SSR markers uniformly covering all the D-genome chromosomes. Different marker systems mostly detected different lines with deletions. Using markers covering known distances, the mapping resolution of this RH panel was estimated to be <140kb. Analysis of only 16 RH lines carrying deletions on chromosome 2D resulted in a physical map with cM/cR ratio of 1:5.2 and 15 distinct bins. Additionally, with this small set of lines, almost all the tested ESTs could be mapped. A set of 399 most informative RH lines with an average deletion frequency of ~10% were identified for developing high density marker scaffolds of the D-genome.ConclusionsThe RH panel reported here is the first developed for any wild ancestor of a major cultivated plant species. The results provided insight into various aspects of RH mapping in plants, including the genetically effective cell number for wheat (for the first time) and the potential implementation of this technique in other plant species. This RH panel will be an invaluable resource for mapping gene based markers, developing a complete marker scaffold for the whole genome sequence assembly, fine mapping of markers and functional characterization of genes and gene networks present on the D-genome.

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“…Spring wheat Choteau (6X) and the durum wheat Mountrail (4X) were found to produce a sufficient amount of viable seed to develop recombinant inbred line (RIL) populations at both 4X and 6X ploidy levels. Most of the progeny in advanced generations were euploid , as was observed in another set of 6X/4X crosses (Martin et al 2011). The development of 4X and 6X RIL populations from crosses of 4X and 6X parents gives the opportunity to determine the effect of the D genome on quantitative traits in a wheat population, including epistatic interactions.…”

Section: Introductionmentioning

confidence: 99%

Impact of the D genome and quantitative trait loci on quantitative traits in a spring durum by spring bread wheat cross

et al. 2015

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The impact of the D genome and QTL in the A and B genomes on agronomic performance of hexaploid wheat and tetraploid durum was determined using novel recombinant inbred line populations derived from interploid crosses. Genetic differences between common hexaploid (6X) bread wheat (Triticum aestivum, 2n = 6x = 42, genome, AABBDD) and tetraploid (4X) durum wheat (T. turgidum subsp. durum, 2n = 4x = 28, genome, AABB) may exist due to effects of the D genome and allelic differences at loci in the A and B genomes. Previous work allowed identification of a 6X by 4X cross combination that resulted in a large number of fertile recombinant progeny at both ploidy levels. In this study, interspecific recombinant inbred line populations at both 4X and 6X ploidy with 88 and 117 individuals, respectively, were developed from a cross between Choteau spring wheat (6X) and Mountrail durum wheat (4X). The presence of the D genome in the 6X population resulted in increased yield, tiller number, kernel weight, and kernel size, as well as a decrease in stem solidness, test weight and seed per spike. Similar results were found with a second RIL population containing 152 lines from 18 additional 6X by 4X crosses. Several QTL for agronomic and quality traits were identified in both the 4X and 6X populations. Although negatively impacted by the lack of the D genome, kernel weight in Mountrail (4X) was higher than Choteau (6X) due to positive alleles from Mountrail on chromosomes 3B and 7A. These and other favorable alleles may be useful for introgression between ploidy levels.

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Retention of D genome chromosomes in pentaploid wheat crosses

Cited by 18 publications

References 16 publications

The Capacity to Buffer and Sustain Imbalanced D-Subgenome Chromosomes by the BBAA Component of Hexaploid Wheat Is an Evolved Dominant Trait

The Capacity to Buffer and Sustain Imbalanced D-Subgenome Chromosomes by the BBAA Component of Hexaploid Wheat Is an Evolved Dominant Trait

Physical mapping resources for large plant genomes: radiation hybrids for wheat D-genome progenitor Aegilops tauschii

Impact of the D genome and quantitative trait loci on quantitative traits in a spring durum by spring bread wheat cross

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