Retention of bacteria by cellulose fibres as a means of reducing biofouling in paper pulp production processes

Vieira, M. J.; Beleza, V. M.; Melo, L. F.

doi:10.1080/08927019809378367

Cited by 8 publications

(5 citation statements)

References 14 publications

(16 reference statements)

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“…A continuous culture of P. fluorescens was produced as described elsewhere (Pereira et al, 1998). The bacterial culture was grown in a 0.5 l glass reactor suitably aerated and agitated, and continuously fed with a sterile concentrated nutrient solution consisting of 5 g glucose l 71 , 2.5 g peptone l 71 and 1.25 g yeast extract l 71 , in phosphate buffer at pH 7, at a flow rate of 10 ml h 71 .…”

Section: Microorganism and Cell Growthmentioning

confidence: 99%

Effect of Different Concentrations ofOrtho-phthalaldehyde on Biofilms Formed byPseudomonas fluorescensUnder Different Flow Conditions

Simões¹,

Vieira²

2003

Biofouling

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The effectiveness of different concentrations of ortho-phthalaldehyde (OPA) in controlling biofilms of Pseudomonas fluorescens formed on stainless steel slides, using flow cell reactors under laminar and turbulent flow, was investigated by determining the variation in mass and respiratory activity. The physical stability of the biofilm with and without exposure to OPA was studied in a rotating device as variation in the mass of the biofilm on the surface after exposure to different rotation velocities. The activity of OPA against bacterial suspended cultures was evaluated in the presence and absence of bovine serum albumin (BSA) in order to evaluate the interference of proteins on the activity of the biocide. The results showed that biofilms formed under different flow conditions had different properties and reacted differently after biocide application. Biofilms formed under laminar flow were more easily inactivated than those formed under turbulent conditions. However, OPA did not promote the detachment of biofilms from the surface. The exposure of biofilms to different shear stress conditions after OPA treatment enhanced removal from the surface, indicating that OPA may weaken the biofilm matrix. The biocide was more effective on suspended cells than on cells grown in biofilms. This fact may be explained by the reaction of the biocide with proteins of the polymeric matrix of the biofilm as suggested by the significant reduction of biocide action on suspended cells in the presence of BSA.

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Section: Microorganism and Cell Growthmentioning

confidence: 99%

Effect of Different Concentrations ofOrtho-phthalaldehyde on Biofilms Formed byPseudomonas fluorescensUnder Different Flow Conditions

Simões¹,

Vieira²

2003

Biofouling

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“…Microorganism growth. A continuous pure culture of the P. fluorescens bacteria as described elsewhere (Pereira et al, 1998) was used as the test microorganism. A continuous pure culture of the bacteria was grown in a 2 L glass fermenter, at 27 ± 1°C, suitably aerated and magnetically agitated.…”

Section: Experiments With Planktonic Bacteriamentioning

confidence: 99%

“…The respiratory activity of the several samples was evaluated by measuring oxygen uptake rates in a biological oxygen monitor (BOM) in short-term assays. The assays were performed in a Yellow Springs Instruments BOM (Model 53) and the procedure used has been described elsewhere (Pereira et al, 1998).…”

Section: Experiments With Planktonic Bacteriamentioning

confidence: 99%

Monitoring the effects of biocide treatment of Pseudomonas fluorescens biofilms formed under different flow regimes

Simões

Vieira

2003

Water Science and Technology

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The effectiveness of glutaraldehyde (GTA), a very common biocide for controlling biofilms formed by Pseudomonas fluorescens on stainless steel slides, in laminar and turbulent flow, was investigated. Tests were performed using a concentration of biocide of 200 mg L(-1) and a range of exposure times. The GTA action was assessed by means of activity tests and dry weight of the biofilms. The physical stability of the biofilm without biocide application and after exposure to GTA for different periods of time was also studied and evaluated through the variation of the mass of the deposit after submission to different rotation velocities. The results showed that, in all the situations studied, biofilms were not controlled after the treatment with biocide, since they remained on the metal surface and were still active. The results also demonstrated that the physical stability of the biofilms increased with biocide application.

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“…Microorganism growth A continuous pure culture of P. fluorescens was produced as described elsewhere (Pereira et al, 1998). The bacterial culture was grown at 27+18C in a 3 l glass fermenter, suitably aerated and magnetically agitated.…”

Section: Experiments With Suspended Microorganismsmentioning

confidence: 99%

Studies on the Behaviour of Pseudomonas fluorescens Biofilms after Ortho-phthalaldehyde Treatment

Simões¹,

Carvalho²,

Vieira³

2003

GBIF

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A relatively novel biocide, ortho-phthalaldehyde (OPA), was tested to control biofilms formed by Pseudomonas fluorescens on stainless steel surfaces. The toxic action of OPA was assessed in terms of inactivation and removal of the biofilm by means of, respectively, the determination of the respiratory activity and the variation in the dry weight of the biofilms. For comparison, the activity of OPA against suspended bacteria was also evaluated. The results showed that higher concentrations of OPA and longer exposure times are needed to inactivate P. fluorescens biofilms than planktonic populations, thus denoting that sessile bacteria have a reduced susceptibility to OPA. This appears to be associated with the reaction with the proteins of the matrix, as demonstrated by the reduction of the antimicrobial action of OPA in the presence of a protein (bovine serum albumin). The application of OPA appeared to cause little effect in the removal of biofilms from the metal slides since the mass of biofilm that remained on the surfaces, after biocide treatment, was within the same range as those observed in the control tests. These results suggest that, with OPA application, biofilms can be inactivated but stay attached to the surfaces, decreasing thereby the success of the chemical treatment.

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Retention of bacteria by cellulose fibres as a means of reducing biofouling in paper pulp production processes

Cited by 8 publications

References 14 publications

Effect of Different Concentrations ofOrtho-phthalaldehyde on Biofilms Formed byPseudomonas fluorescensUnder Different Flow Conditions

Effect of Different Concentrations ofOrtho-phthalaldehyde on Biofilms Formed byPseudomonas fluorescensUnder Different Flow Conditions

Monitoring the effects of biocide treatment of Pseudomonas fluorescens biofilms formed under different flow regimes

Studies on the Behaviour of Pseudomonas fluorescens Biofilms after Ortho-phthalaldehyde Treatment

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