Retention and separation efficiency of some synthetic oligopeptides in reversed-phase thin-layer chromatography

Gwarda, Radosław Ł.; Tomczyszyn, Aleksandra; Misicka, Aleksandra; Dzido, Tadeusz H.

doi:10.1556/achrom.27.2015.1.1

Cited by 12 publications

(2 citation statements)

References 15 publications

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“…It is a cost-effective method as many samples can be analyzed simultaneously with only small solvent consumption. In addition, various stationary and (gradient) mobile phases as well as detection options are available [ 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. Through the application of fingerprinting, HPTLC can also provide a simple and reliable method for quality control.…”

Section: Introductionmentioning

confidence: 99%

High-Performance Thin-Layer Chromatography-Immunostaining as a Technique for the Characterization of Whey Protein Enrichment in Edam Cheese

Treblin

Oesen

Lüneburg

et al. 2022

Foods

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Whey protein-enriched cheese can be produced by means of a high-temperature treatment of a part of the cheese milk. In this way, the nutritional quality of the resulting cheeses can be increased while resources are conserved. High-performance thin-layer chromatography-immunostaining (HPTLC-IS) using specific β-lactoglobulin (β-LG) antibodies was applied to study the implementation and stability of β-LG in two different sample sets of whey protein-enriched Edam model cheeses, including industrial-scale ones. Two methods were compared for the extraction of the proteins/peptides from the cheese samples. By applying tryptic hydrolysis directly from a suspended cheese sample instead of a supernatant of a centrifuged suspension, a better yield was obtained for the extraction of β-LG. When applying this method, it was found that selected epitopes in the tryptic β-LG peptides remain stable over the ripening period of the cheese. For four of the tryptic β-LG peptides detected by immunostaining, the amino acid sequence was identified using MALDI-TOF-MS/MS. One of the peptides identified was the semi-tryptic peptide VYVEELKPTP. A linear relationship was found between the content of this peptide in cheese and the proportion of high-heated milk in the cheese milk. β-LG enrichment factors of 1.72 (n = 3, sample set I) and 1.33 ± 0.19 (n = 1, sample set II) were determined for the cheese samples containing 30% high-heated milk compared to the non-enriched samples. The relative β-LG contents in the cheese samples with 30% high-heated milk were calculated to be 4.35% ± 0.39% (sample set I) and 9.11% ± 0.29% (sample set II) using a one-point calibration. It can be concluded that the HPTLC-IS method used is a suitable tool for the analysis of whey protein accumulation in cheese, being therefore potentially directly applicable on an industrial scale. For more accurate quantification of the whey protein content in cheese, an enhanced calibration curve needs to be applied.

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Section: Introductionmentioning

confidence: 99%

High-Performance Thin-Layer Chromatography-Immunostaining as a Technique for the Characterization of Whey Protein Enrichment in Edam Cheese

Treblin

Oesen

Lüneburg

et al. 2022

Foods

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“…In our previous papers we have presented detailed investigations concerning influence of particular variables on selectivity and efficiency of peptide separation in HPTLC [4,6,18,19] and PPEC [4,20] systems, under various conditions. The aim of this work was the correlation of the selectivity obtained using similar HPTLC and PPEC separation systems studied before and evaluation of potential utility of combination of these both techniques for 2D separation of peptides.…”

Section: Introductionmentioning

confidence: 99%

Correlation of Migration Distance of Peptides in High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography Systems

Gwarda

Dzido

2018

Chromatographia

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In a series of our previous papers we have investigated the influence of various variables on retention/migration of peptides in various high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC) systems. Here we present a correlation of the selectivity of peptide separation in similar, as well as in various HPTLC and PPEC systems investigated before. Our results show that the selectivity in similar HPTLC and PPEC systems is quite different. This results from the share of electrophoresis in separation of solutes by PPEC. The results suggest that combination of HPTLC and PPEC, with properly selected separation conditions (the same, or even better-different for each technique), may be used for efficient two-dimensional separation of peptides. The best separation can be obtained if PPEC is carried out in two opposite directions (toward the cathode and the anode) simultaneously.

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Pressurized planar electrochromatography of DNS amino acids derivatives in silica gel and silanized silica gel systems with formic acid addition to the water mobile phase

Chomicki

Dzido

2021

JPC-J Planar Chromat

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Pressurized planar electrochromatography (PPEC) of dansyl (DNS) derivatives of amino acids in normal- and reversed-phase systems is presented. The results have been obtained for mobile phases with different acetonitrile (ACN) concentrations (0–85%). The data obtained show differences in separation selectivity between high-performance thin-layer chromatography (HPTLC) and PPEC systems. These differences originate from the electrophoretic effect which is involved in the PPEC system, contrary to the HPTLC one.

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Retention and separation efficiency of some synthetic oligopeptides in reversed-phase thin-layer chromatography

Cited by 12 publications

References 15 publications

High-Performance Thin-Layer Chromatography-Immunostaining as a Technique for the Characterization of Whey Protein Enrichment in Edam Cheese

High-Performance Thin-Layer Chromatography-Immunostaining as a Technique for the Characterization of Whey Protein Enrichment in Edam Cheese

Correlation of Migration Distance of Peptides in High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography Systems

Pressurized planar electrochromatography of DNS amino acids derivatives in silica gel and silanized silica gel systems with formic acid addition to the water mobile phase

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