“…Dmitry Shayakhmetov and Andre Lieber (University of Washington) (Shayakhmetov and Lieber, 2000). The modified fibre gene was subcloned into an E1/E3-deleted Ad genomic plasmid, pRP2014 (Christou and Parks, 2011;Poulin et al, 2010), and a cytomegalovirus immediate early enhancer/promoter-lacZ expression cassette (pCA38, (Addison et al, 1997)) was introduced to replace the E1-deletion, using RecA-mediated recombination (Chartier et al, 1996), as detailed previously (Willemsen, 2009), and designated Ad5SlacZ. To introduce the poly-lysine motif into the H-I loop of the knob domain of the short fibre, a BglII/PstI fragment containing part of the knob domain was first subcloned into pSP72 (Promega).…”