Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression

Liao, Yiling; Chiou, Ming-Jyun; Tsai, Jia-Lun; Wen, Chi‐Chung; Wang, Y.-H.; Cheng, Chen; Chen, Y.-H.

doi:10.38212/2224-6614.2258

Cited by 4 publications

(3 citation statements)

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“…DaiDai fruit is abundant in flavonoids and polyphenols, known for their antioxidant and anti-inflammatory properties. These ingredients not only suppress the generation of inflammatory cells in models of inflammation but also reduce the expression of associated inflammatory factors, leading to anti-inflammatory effects ( Liao et al, 2020 ; Russo et al, 2023 ). The fermentation process increased the levels of flavonoids and polyphenolic ingredients in the DaiDai fruit fermentation broth, enhancing its anti-inflammatory potential.…”

Section: Resultsmentioning

confidence: 99%

Fermentation of DaiDai fruit and its biological activity

Meng,

Tang,

et al. 2024

Front. Microbiol.

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DaiDai fruit, a medicinal and edible plant fruit, is abundant in biologically active compounds and has a long history of use in traditional Chinese medicine. This research focuses on utilizing fermentation to develop a functional DaiDai fruit fermentation broth. Lactobacillus, Bacillus subtilis and Saccharomyces cerevisiae were employed in the fermentation process. By conducting screenings of bacterial strains, single factor experiments, and response surface methodology, the total flavonoids, polysaccharides, polyphenols, and 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical scavenging rate were used as the index for selection, ultimately identifying Lactobacillus L-13 as the optimal fermentation strain. The optimal fermentation conditions were determined to be a time of 108 h, a temperature of 43.6°C, and a solid–liquid ratio of 1:15.157 (w/v). Under these conditions, the total flavonoid content reached 412.01 mg/g, representing a 36.71% increase compared to conventional extraction methods. The contents of polysaccharides and polyphenols and the DPPH scavenging rate were also increased. The fermentation broth of DaiDai fruit exhibited inhibitory effects on tyrosinase and melanin production in mouse melanoma cells B16-F10 induced by α-MSH and anti-inflammatory properties in a zebrafish inflammation model. These indicate that the DaiDai fruit fermentation broth possesses anti-melanoma, whitening, and anti-inflammatory properties, showcasing significant potential for applications in medicine, cosmetics, and other industries.

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Section: Resultsmentioning

confidence: 99%

Fermentation of DaiDai fruit and its biological activity

Meng,

Tang,

et al. 2024

Front. Microbiol.

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“…Zebrafish embryo is also accepted as a proper model for assessing the anti-inflammatory effect of drugs because zebrafish has innate and acquired immune systems similar to mammals (Hwang et al., 2016 ; S. Lee et al., 2013 ). Also, the zebrafish embryos can develop immune cells similar to their mammalian equivalents (Liao et al., 2011 ), which makes the inflammatory pathophysiological features of zebrafish similar to human (Yang et al., 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Fabrication and characterization of Agarwood extract-loaded nanocapsules and evaluation of their toxicity and anti-inflammatory activity on RAW 264.7 cells and in zebrafish embryos

et al. 2021

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Aquilaria malaccensis has been traditionally used to treat several medical disorders including inflammation. However, the traditional claims of this plant as an anti-inflammatory agent has not been substantially evaluated using modern scientific techniques. The main objective of this study was to evaluate the anti-inflammatory effect of Aquilaria malacensis leaf extract (ALEX-M) and potentiate its activity through nano-encapsulation. The extract-loaded nanocapsules were fabricated using water-in-oil-in-water ( w/o/w ) emulsion method and characterized via multiple techniques including DLS, TEM, FTIR, and TGA. The toxicity and the anti-inflammatory activity of ALEX-M and the extract-loaded nanocapsules (ALEX-M-PNCs) were evaluated in-vitro on RAW 264.7 macrophages and in-vivo on zebrafish embryos. The nanocapsules demonstrated spherical shape with mean particle diameter of 167.13 ± 1.24 nm, narrow size distribution (PDI = 0.29 ± 0.01), and high encapsulation efficiency (87.36 ± 1.81%). ALEX-M demonstrated high viability at high concentrations in RAW 264.7 cells and zebrafish embryos, however, ALEX-M-PNCs showed relatively higher cytotoxicity. Both free and nanoencapsulated extract expressed anti-inflammatory effects through significant reduction of the pro-inflammatory mediator nitric oxide (NO) production in LPS/IFNγ-stimulated RAW 264.7 macrophages and zebrafish embryos in a concentration-dependent manner. The findings highlight that ALEX-M can be recognized as a potential anti-inflammatory agent, and its anti-inflammatory activity can be potentiated by nano-encapsulation. Further studies are warranted toward investigation of the mechanistic and immunomodulatory roles of ALEX-M.

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“…Therefore, zebrafish are a useful and popular animal model for a variety of inflammation studies. In in vivo anti-inflammation test models, examining zebrafish is widely accepted as the best method for effective anti-inflammation assay [ 19 , 20 , 21 ]. Therefore, in this study, extract of soft coral Dendronephthya puetteri (DPE) was investigated to identify in vivo anti-inflammatory effects in zebrafish model for its potential use in natural anti-inflammatory agent.…”

Section: Introductionmentioning

confidence: 99%

Soft Coral Dendronephthya puetteri Extract Ameliorates Inflammations by Suppressing Inflammatory Mediators and Oxidative Stress in LPS-Stimulated Zebrafish

Kim

Ding

Yang

et al. 2018

IJMS

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Marine-derived extract and/or bioactive compounds have attracted increasing demand due to their unique and potential uses as cures for various inflammation-based diseases. Several studies revealed anti-inflammatory candidates found in soft corals. However, the effects of soft corals on inflammation in an in vivo model remain to be determined. Therefore, the extract of soft coral Dendronephthya puetteri (DPE) was investigated for an in vivo anti-inflammatory effect in a lipopolysaccharide (LPS)-stimulated zebrafish model to determine its potential use as a natural anti-inflammatory agent. We also investigated whether DPE has toxic effects in a zebrafish model. No significant changes were observed in terms of survival, heart beat rate, or developmental abnormalities in the zebrafish embryos exposed to a concentration below 100 µg/mL of DPE. Treating the zebrafish model with LPS-treatment significantly increased the ROS, NO generation, and cell death. However, DPE inhibited this LPS-stimulated ROS, NO generation, and cell death in a dose-dependent manner. In addition, DPE significantly reduced the mRNA expression of both iNOS and COX-2 and markedly suppressed the expression levels of the proinflammatory cytokines, TNF-α and IL-6, in an LPS-stimulated zebrafish model. These findings demonstrate that DPE has profound anti-inflammatory effect in vivo, suggesting that DPE might be a strong natural anti-inflammatory agent.

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Resveratrol treatment attenuates the wound-induced inflammation in zebrafish larvae through the suppression of myeloperoxidase expression

Cited by 4 publications

References 0 publications

Fermentation of DaiDai fruit and its biological activity

Fermentation of DaiDai fruit and its biological activity

Fabrication and characterization of Agarwood extract-loaded nanocapsules and evaluation of their toxicity and anti-inflammatory activity on RAW 264.7 cells and in zebrafish embryos

Soft Coral Dendronephthya puetteri Extract Ameliorates Inflammations by Suppressing Inflammatory Mediators and Oxidative Stress in LPS-Stimulated Zebrafish

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