Resveratrol supplementation of high‐fat diet‐fed pregnant mice promotes brown and beige adipocyte development and prevents obesity in male offspring

Abstract

Promoting beige/brite adipogenesis and thermogenic activity is considered as a promising therapeutic approach to reduce obesity and metabolic syndrome. Maternal obesity impairs offspring brown adipocyte function and correlates with obesity in offspring. We previously found that dietary resveratrol (RES) induces beige adipocyte formation in adult mice. Here, we evaluated further the effect of resveratrol supplementation of pregnant mice on offspring thermogenesis and energy expenditure. Female C57BL/6 J mice were fed a control diet (CON) or a high-fat diet (HFD) with or without 0.2% (w/w) RES during pregnancy and lactation. Male offspring were weaned onto a HFD and maintained on this diet for 11 weeks. The offspring thermogenesis and related regulatory factors in adipose tissue were evaluated. At weaning, HFD offspring had lower thermogenesis in brown and white adipose tissues compared with CON offspring, which was recovered by maternal RES supplementation, along with the appearance of multilocular brown/beige adipocytes and elevated thermogenic gene expression. Adult offspring of RES-treated mothers showed increased energy expenditure and insulin sensitivity when on an obesogenic diet compared with HFD offspring. The elevated metabolic activity was correlated with enhanced brown adipose function and white adipose tissue browning in HFD+RES compared with HFD offspring. In conclusion, RES supplementation of HFD-fed dams during pregnancy and lactation promoted white adipose browning and thermogenesis in offspring at weaning accompanied by persistent beneficial effects in protecting against HFD-induced obesity and metabolic disorders.

Programming of the Beige Phenotype in White Adipose Tissue of Adult Mice by Mild Resveratrol and Nicotinamide Riboside Supplementations in Early Postnatal Life

“…Two recent reports carried out in mice and rats studied whether perinatal RSV supplementation to pregnant and lactating mothers could counteract undesired effects of maternal malnutrition in the offspring. In these studies, maternal RSV supplementation had no effect on the offspring of regular diet‐fed dams but counteracted the increased adiposity at weaning of the offspring of HFD‐fed dams .…”

“…Two recent reports carried out in mice and rats studied whether perinatal RSV supplementation to pregnant and lactating mothers could counteract undesired effects of maternal malnutrition in the offspring. In these studies, maternal RSV supplementation had no effect on the offspring of regular diet‐fed dams but counteracted the increased adiposity at weaning of the offspring of HFD‐fed dams . The study carried out in mice addressed long‐term effects, showing that maternal high RSV supplementation (≈200 mg kg –1 body weight per day) also counteracts the increased susceptibility of the offspring of HFD‐fed dams to develop obesity upon HFD feeding post‐weaning through effects in adipose tissues .…”

“…In these studies, maternal RSV supplementation had no effect on the offspring of regular diet‐fed dams but counteracted the increased adiposity at weaning of the offspring of HFD‐fed dams . The study carried out in mice addressed long‐term effects, showing that maternal high RSV supplementation (≈200 mg kg –1 body weight per day) also counteracts the increased susceptibility of the offspring of HFD‐fed dams to develop obesity upon HFD feeding post‐weaning through effects in adipose tissues . However, only male offspring was studied, and interpretation of the results is complicated, due to the fact that a pharmacological dose of RSV was used, RSV can cross the placenta, and little is known about its excretion in breast milk.…”

“…However, only male offspring was studied, and interpretation of the results is complicated, due to the fact that a pharmacological dose of RSV was used, RSV can cross the placenta, and little is known about its excretion in breast milk. Thus, in the study of Zou et al., direct effects in the fetus cannot be discarded, as well as effects due to changes in milk composition secondary to the high maternal RSV intake.…”

Prevalence of generalized and abdominal obesity in police worker - A cross-sectional study

“…[1][2][3] It has been defined by the World Health Organization (WHO) as abnormal or excessive fat accumulation that may impair health. Obesity is one of the most common, yet, among the most neglected, public health problems in both developed and developing countries.…”