Resveratrol Regulates Mitochondrial Biogenesis and Fission/Fusion to Attenuate Rotenone‐Induced Neurotoxicity

Peng, Kaige; Yuan, Tao; Zhang, Jun; Wang, Jian; Feng, Yang; Dan, Guorong; Zhao, Yuanpeng; Cai, Ying; Zhao, Jinmin; Wu, Qiang; Zou, Zhongmin; Cao, Jia; Sai, Yan

doi:10.1155/2016/6705621

Cited by 99 publications

(63 citation statements)

References 33 publications

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“…In addition, this study showed that resveratrol treatments increase both mitochondrial mass and mtDNA contents during osteogenic differentiation in PO-MSCs. These findings indicate that resveratrol can up-regulate mitochondrial biogenesis during osteogenic differentiation of PO-MSCs and are consistent with other reports regarding mitochondrial biogenesis by resveratrol in various cell types (26,(38)(39)(40)(41)(42). Moreover, the up-regulation of mitochondrial biogenesis by resveratrol in PO-MSCs is in line with the notion that metabolic shift from glycolysis to mitochondrial OXPHOS occurs during stem cell differentiation (16,17,(43)(44)(45)(46)(47).…”

Section: Discussionsupporting

confidence: 92%

Resveratrol can enhance osteogenic differentiation and mitochondrial biogenesis from human periosteum-derived mesenchymal stem cells

Moon

Kim

Lee

et al. 2020

Preprint

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Background: Osteoporosis is a metabolic bone disorder that leads to low bone mass and microstructural deterioration of bone tissue and increases bone fractures. Resveratrol, a natural polyphenol compound, has pleiotropic effects including anti-oxidative, anti-aging, and anti-cancer effects. Resveratrol also has roles in increasing osteogenesis and in up-regulating mitochondrial biogenesis of bone marrow-derived mesenchymal stem cells (BM-MSCs). However, it is still unclear that resveratrol can enhance osteogenic differentiation or mitochondrial biogenesis of periosteum-derived MSCs (PO-MSCs), which play key roles in bone tissue maintenance and fracture healing. Thus, in order to test a possible preventive or therapeutic effect of resveratrol on osteoporosis, this study investigated effects of resveratrol treatments on osteogenic differentiation and mitochondrial biogenesis of PO-MSCs.Methods: The optimal doses of resveratrol treatment on PO-MSCs were determined by cell proliferation and viability assays. Osteogenic differentiation of PO-MSCs under resveratrol treatment was assessed by alkaline phosphatase activities (ALP, an early biomarker of osteogenesis) as well as by extracellular calcium deposit levels (a late biomarker). Mitochondrial biogenesis during osteogenic differentiation of PO-MSCs was measured by quantifying both mitochondrial mass and mitochondrial DNA (mtDNA) contents.Results: Resveratrol treatments above 10 µM seem to have negative effects on cell proliferation and viability of PO-MSCs. Resveratrol treatment (at 5 µM) on PO-MSCs during osteogenic differentiation increased both ALP activities and calcium deposits compared to untreated control groups, demonstrating an enhancing effect of resveratrol on osteogenesis. In addition, resveratrol treatment (at 5 µM) during osteogenic differentiation of PO-MSCs increased both mitochondrial mass and mtDNA copy numbers, indicating that resveratrol can bolster mitochondrial biogenesis in the process of PO-MSC osteogenic differentiation.Conclusion: Taken together, the findings of this study describe roles of resveratrol in promoting osteogenesis and mitochondrial biogenesis of human PO-MSCs suggesting a possible application of resveratrol as a supplement for osteoporosis and/or osteoporotic fractures.

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Section: Discussionsupporting

confidence: 92%

Resveratrol can enhance osteogenic differentiation and mitochondrial biogenesis from human periosteum-derived mesenchymal stem cells

Moon

Kim

Lee

et al. 2020

Preprint

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“…The cells showing 70% fragmented or intermediate mitochondria were classified as fragmented cells [42]; their number increased significantly upon infliction of oxidative treatment and, not surprisingly, Cr preloading prevented this effect (Figure 1(d)). …”

Section: Resultsmentioning

confidence: 99%

Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity

Barbieri

Guescini

Calcabrini

et al. 2016

Oxidative Medicine and Cellular Longevity

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Creatine (Cr) is a nutritional supplement promoting a number of health benefits. Indeed Cr has been shown to be beneficial in disease-induced muscle atrophy, improve rehabilitation, and afford mild antioxidant activity. The beneficial effects are likely to derive from pleiotropic interactions. In accord with this notion, we previously demonstrated that multiple pleiotropic effects, including preservation of mitochondrial damage, account for the capacity of Cr to prevent the differentiation arrest caused by oxidative stress in C2C12 myoblasts. Given the importance of mitochondria in supporting the myogenic process, here we further explored the protective effects of Cr on the structure, function, and networking of these organelles in C2C12 cells differentiating under oxidative stressing conditions; the effects on the energy sensor AMPK, on PGC-1α, which is involved in mitochondrial biogenesis and its downstream effector Tfam were also investigated. Our results indicate that damage to mitochondria is crucial in the differentiation imbalance caused by oxidative stress and that the Cr-prevention of these injuries is invariably associated with the recovery of the normal myogenic capacity. We also found that Cr activates AMPK and induces an upregulation of PGC-1α expression, two events which are likely to contribute to the protection of mitochondrial quality and function.

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“…Type I (slow-twitch) muscle fibres are rich in the mitochondria (3,32) . It has been reported that some nutrients (such as leucine and resveratrol) can regulate type I (slowtwitch) muscle fibres expression by improving the mitochondrial biogenesis and function (29,30,33) . In the present study, our data found that dietary supplementation of 1•0 % Arg significantly increased the PGC-1α, Sirt1 and Cytc protein expressions, the PGC-1α, NRF1, TFB1M, Cytc and ATP5G mRNA expressions and the mtDNA content, suggesting that Arg plays an important role in regulating mitochondrial biogenesis and function.…”

Section: Discussionmentioning

confidence: 99%

Arginine promotes porcine type I muscle fibres formation through improvement of mitochondrial biogenesis

et al. 2019

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The present study aimed to investigate whether arginine (Arg) promotes porcine type I muscle fibres formation via improving mitochondrial biogenesis. In the in vivo study, a total of sixty Duroc × Landrace × Yorkshire weaning piglets with an average body weight of 6·55 (sd 0·36) kg were randomly divided into four treatments and fed with a basal diet or a basal diet supplemented with 0·5, 1·0 and 1·5 % l-Arg, respectively, in a 4-week trial. Results showed that dietary supplementation of 1·0 % Arg significantly enhanced the activity of succinate dehydrogenase, up-regulated the protein expression of myosin heavy chain I (MyHC I) and increased the mRNA levels of MyHC I, troponin I1, C1 and T1 (Tnni1, Tnnc1 and Tnnt1) in longissimus dorsi muscle compared with the control group. In addition, ATPase staining analysis indicated that 1·0 % Arg supplementation significantly increased the number of type I muscle fibres and significantly decreased the number of type II muscle fibres. Furthermore, 1·0 % Arg supplementation significantly up-regulated PPAR-γ coactivator-1α (PGC-1α), sirtuin 1 and cytochrome c (Cytc) protein expressions, increased PGC-1α, nuclear respiratory factor 1 (NRF1), mitochondria transcription factor B1 (TFB1M), Cytc and ATP synthase subunit C1 (ATP5G) mRNA levels and increased mitochondrial DNA content. In the in vitro study, mitochondrial complex I inhibitor rotenone (Rot) was used. We found that Rot annulled Arg-induced type I muscle fibres formation. Together, our results provide for the first time the evidence that Arg promotes porcine type I muscle fibres formation through improvement of mitochondrial biogenesis.

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Resveratrol Regulates Mitochondrial Biogenesis and Fission/Fusion to Attenuate Rotenone‐Induced Neurotoxicity

Cited by 99 publications

References 33 publications

Resveratrol can enhance osteogenic differentiation and mitochondrial biogenesis from human periosteum-derived mesenchymal stem cells

Resveratrol can enhance osteogenic differentiation and mitochondrial biogenesis from human periosteum-derived mesenchymal stem cells

Creatine Prevents the Structural and Functional Damage to Mitochondria in Myogenic, Oxidatively Stressed C2C12 Cells and Restores Their Differentiation Capacity

Arginine promotes porcine type I muscle fibres formation through improvement of mitochondrial biogenesis

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