Resveratrol raises in vitro anticancer effects of paclitaxel in NSCLC cell line A549 through COX-2 expression

Abstract

The aim of this study was to determine the raising anticancer effects of resveratrol (Res) on paclitaxel (PA) in non-small cell lung cancer (NSCLC) cell line A549. The 10 µg/ml of Res had no effect on human fetal lung fibroblast MRC-5 cells or on A549 cancer cells and the 5 or 10 µg/ml of PA also had no effect on MRC-5 normal cells. PA-L (5 µg/ml) and PA-H (10 µg/ml) had the growth inhibitory effects in NSCLC cell line A549, and Res increased these growth inhibitory effects. By flow cytometry experiment, after Res (5 µg/ml)+PA-H (10 µg/ml) treatment, the A549 cells showed the most apoptosic cells compared to other group treatments, and after additional treatment with Res, the apoptosic cells of both two PA concentrations were raised. Res+PA could reduce the mRNA and protein expressions of COX-2, and Res+PA could reduce the COX-2 related genes of VEGF, MMP-1, MMP-2, MMP-9, NF-κB, Bcl-2, Bcl-xL, procollagen I, collagen I, collagen III and CTGF, TNF-α, IL-1β, iNOS and raise the TIMP-1, TIMP-2, TIMP-3, IκB-α, p53, p21, caspase-3, caspase-8, caspase-9, Bax genes compared to the control cells and the PA treated cells. From these results, it can be suggested that Res could raise the anticancer effects of PA in A549 cells, thus Res might be used as a good sensitizing agent for PA.

Resveratrol Enhances Apoptotic and Oxidant Effects of Paclitaxel through TRPM2 Channel Activation in DBTRG Glioblastoma Cells

“…Hence, this combination enhanced apoptotic effects in DBTRG cells. Similarly, another study showed that RESV increased the anticancer effects of PAX through upregulation of apoptosis and caspase 3 in non-small cell lung cancer [ 86 ] and HepG2 cancer cell lines [ 12 , 87 , 88 ]. Our data shows that PAX and RESV treatment enhances apoptosis in a caspase-9-independent manner.…”

Resveratrol: A Double-Edged Sword in Health Benefits

“…Stilbenoids including resveratrol are non-nitrogenous polyphenols with acidic and amphiphilic characters with anti-inflammatory activity. Many of their targets are occurring on cyclooxygenase (COX), 5-lipoxygenase (5-LOX) and protein kinase B [ 92 ], which is associated with its ability to inhibit COX-1 and COX-2 activity along with transcription factors activity inhibition, directly involved in COX activity regulation [ 93 ]. Studies reported the ability of resveratrol to reduce the secretion and expression of inflammatory factors [ 94 ].…”

Synthesis and Cytotoxic Activities of Resveratrol and Valproic Acid Ester Derivatives with Anti-Bladder-Cancer Effects

“…Previous studies have shown that lauric acid and resveratrol have anticancer effects and are useful in the management of various cancers. [10][11][12][13][14][15][16][17][26][27][28] . Compound 2 is a synthetic substance, synthesized from lauric acid and resveratrol.…”