Resveratrol Protects Mouse Oocytes from Methylglyoxal-Induced Oxidative Damage

Abstract

Methylglyoxal, a reactive dicarbonyl compound, is mainly formed from glycolysis. Methylglyoxal can lead to the dysfunction of mitochondria, the depletion of cellular anti-oxidation enzymes and the formation of advanced glycation ends. Previous studies showed that the accumulation of methylglyoxal and advanced glycation ends can impair the oocyte maturation and reduce the oocyte quality in aged and diabetic females. In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. Besides, methylglyoxal-treated oocytes displayed more DNA double strands breaks and this can also be decreased by treatment of resveratrol. Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. Collectively, our results showed that resveratrol can protect the oocytes from methylglyoxal-induced cytotoxicity and this was mainly through the correction of the abnormity of cellular reactive oxygen species metabolism.

The sensitivity of the DNA damage checkpoint prevents oocyte maturation in endometriosis

“…To test if ROS was responsible for the MI arrest associated with Endo-FF, we utilised the antioxidants resveratrol and melatonin353637. Oocytes matured in Endo-FF were >2-fold more likely to undergo PBE in the presence of either 2 μM resveratrol (P < 0.0001; Fig.…”

Resveratrol protects PC12 cells against OGD/R-induced apoptosis via the mitochondrial-mediated signaling pathway

“…Previous studies have demonstrated the neuroprotective properties of resveratrol [6]. For instance, administration of resveratrol to culture medium protects a wide variety of neuronal cell types from oxidative stress-induced injury [7][8][9]; dietary supplementation of resveratrol can ameliorate neuronal damage and death resulted from oxidative stress in rodents [10].…”

“…For instance, edaravone that has been used in the treatment of patients with ischemic stroke inhibits both water-soluble and lipid-soluble peroxyl radical-induced peroxidation systems [5]. Resveratrol (trans-3,4′,5-trihydroxystilbene), a unique phytoalexin widely distributed in natural plants, possesses a strong antioxidant activity capable of opposing the potentially cytotoxic stresses of oxidative damage [6]. Several in vitro studies have identified that resveratrol may protect various neuronal cell types from oxidative stress-induced cell death [7][8][9].…”

YY1 is indispensable for Lgr5 + intestinal stem cell renewal

“…6 A and B). Mitochondrial dysfunction has been shown to increase generation of reactive oxygen species (ROS) and DNA damage (47)(48)(49)(50)(51). Indeed, immunohistochemistry revealed elevated levels of 8-hydroxyguanosine, an antigen formed on DNA oxidation (Fig.…”