Resveratrol protects against oxidative injury induced by H2O2 in acute hippocampal slice preparations from Wistar rats

Almeida, Lúcia Maria Vieira de; Leite, Marina Concli; Thomazi, Ana Paula; Battu, Cintia Eickhoff; Nardin, Patrícia; Tortorelli, Lucas Silva; Zanotto, Caroline; Posser, Thaís; Wofchuk, Susana Tchernin; Leal, Rodrigo Bainy; Gonçalves, Carlos Alberto; Gottfried, Carmem

doi:10.1016/j.abb.2008.09.006

Cited by 56 publications

(32 citation statements)

References 47 publications

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“…However, it remains to be clarified whether these neuroprotective effects in neurons are SIRT1 dependent, since SIRT1 levels decrease during aging and in neurodegenerative disorders such as Alzheimer's disease [40][41][42]. Therefore, the neuroprotective effects of RESV may be due to its antioxidant properties [34].…”

Section: Discussionmentioning

confidence: 98%

“…Currently, SIRT1 activation by RESV is an area of interest, since it leads to improved metabolic control and contributes to protecting cells from several insults such as reactive oxygen species generation and oxidative injury, or from exposure to the neurotoxin 6-hydroxydopamine [32][33][34][35][36][37]. In neuronal cells, SIRT1 induction by RESV contributes to survival in the presence of MPP ?…”

Section: Discussionmentioning

confidence: 99%

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Resveratrol Inhibits Proliferation and Promotes Apoptosis of Neuroblastoma Cells: Role of Sirtuin 1

et al. 2010

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Resveratrol prolongs lifespan and prevent cancer formation; however, the mechanisms are not understood. Here we evaluated the cell-cycle inhibition and apoptosis of resveratrol in B65 neuroblastoma cells, and we also studied the effects of resveratrol on the mammalian silent information regulator 2 (SIRT1). Results show that resveratrol reduces cell viability and causes apoptosis at 24 h of treatment. Resveratrol partially blocked cell proliferation, and significantly increased the fraction of cells arrested in the S phase. The role of SIRT1 in cell-cycle effects mediated by resveratrol was studied through changes in the expression of SIRT1 using western blot. Exposure to resveratrol decreased SIRT1 content, concomitant with an increase in the acetylated form of sirtuin substrates p53 and NFκ-β. Treatment of B65 neuroblastoma cells with resveratrol also reduced the content of the phosphorylated form of AKT. Exposure to the SIRT1 inhibitors nicotinamide and sirtinol altered neither cell viability nor the fraction of apoptotic cells. Furthermore, when cells were exposed simultaneously to resveratrol and nicotinamide or sirtinol, no changes were observed in the fraction of apoptotic cells. Our results show that a decrease in SIRT1 content, caused by exposure to resveratrol, does not appear to be involved in cell-cycle arrest or activation of apoptosis.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Resveratrol Inhibits Proliferation and Promotes Apoptosis of Neuroblastoma Cells: Role of Sirtuin 1

et al. 2010

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“…Hydrogen peroxide (H 2 O 2 ), which is a byproduct of both normal and aberrant metabolism, has been shown to elicit cellular injury by initiating lipid peroxidation, protein oxidation and DNA damage(Cochrane, 1991). At higher concentrations (200-800μM), H 2 O 2 alters astrocyte membranes and the cytoskeleton (Zhu et al, 2005), which enable it to cross cell membranes and reach different subcellular compartments (de Almeida et al, 2008; Pedroso et al, 2009). Inside the cell and in the presence of transition metal ions such as Fe2+ or Cu+, H 2 O 2 can be converted into the highly reactive hydroxyl radical.…”

Section: Discussionmentioning

confidence: 99%

Antioxidative effects of Panax notoginseng saponins in brain cells

et al. 2014

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Oxidative stress resulting from accumulation of reactive oxygen species (ROS) is involved in cell death associated with neurological disorders such as stroke, Alzheimer’s disease and traumatic brain injury. Antioxidant compounds that improve endogenous antioxidant defenses have been proposed for neural protection. The purpose of this study was to investigate the potential protective effects of total saponin in leaves of Panax notoginseng (LPNS) on oxidative stress and cell death in brain cells in vitro. Lactate dehydrogenase (LDH) assay indicated that LPNS (5μg/ml) reduced H2O2-induced cell death in primary rat cortical astrocytes (23±8% reduction in LDH release vs. control). Similar protection was found in oxygen and glucose deprivation/reoxygenation induced SH-SY5Y (a human neuroblastoma cell line) cell damage (78±7% reduction vs.control). The protective effects of LPNS in astrocytes were associated with attenuation of reactive oxygen species (ROS) accumulation. These effects involved activation of Nrf2 (nuclear translocation) and upregulation of downstream antioxidant systems including heme oxygenase-1 (HO-1) and glutathione S-transferase pi 1 (GSTP1). These results demonstrate for the first time that LPNS has antioxidative effects which may be neuroprotectionin neurological disorders.

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“…Although acute OHSCs may be more representative of mature brain than chronic OHSCs, acute slices have been used mostly for electrophysiology studies [148], rather than the examination of pathologic aspects described in this review. Only a few studies have reported using acute slice systems for stroke and TBI research [149–151]. Moreover, acute slices might have their own limitations.…”

Section: Conclusion Limitations and Prospectsmentioning

confidence: 99%

Organotypic Hippocampal Slices as Models for Stroke and Traumatic Brain Injury

Han

Wang

2015

Mol Neurobiol

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Organotypic hippocampal slice cultures (OHSCs) have been used as a powerful ex vivo model for decades. They have been used successfully in studies of neuronal death, microglial activation, mossy fiber regeneration, neurogenesis, and drug screening. As a pre-animal experimental phase for physiologic and pathologic brain research, OHSC offers outcomes that are relatively closer to those of whole-animal studies than outcomes obtained from cell culture in vitro. At the same time, mechanisms can be studied more precisely in OHSCs than they can be in vivo. Here, we summarize stroke and traumatic brain injury research that has been carried out in OHSCs and review classic experimental applications of OHSCs and its limitations.

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Resveratrol protects against oxidative injury induced by H2O2 in acute hippocampal slice preparations from Wistar rats

Cited by 56 publications

References 47 publications

Resveratrol Inhibits Proliferation and Promotes Apoptosis of Neuroblastoma Cells: Role of Sirtuin 1

Resveratrol Inhibits Proliferation and Promotes Apoptosis of Neuroblastoma Cells: Role of Sirtuin 1

Antioxidative effects of Panax notoginseng saponins in brain cells

Organotypic Hippocampal Slices as Models for Stroke and Traumatic Brain Injury

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