Resveratrol promotes the sensitivity of small-cell lung cancer H446 cells to cisplatin by regulating intrinsic apoptosis

Abstract

The aim of the present study was to evaluate the effects of resveratrol on small-cell lung cancer (SCLC) cell proliferation and apoptosis. The results demonstrated that resveratrol concentration- and time-dependently reduced H446 cell viability. In addition, cells treated with resveratrol displayed higher apoptotic rates, in association with mitochondrial depolarization, cytochrome c release from the mitochondrial compartment to the cytoplasm, apoptosis-inducing factor translocation from the mitochondrial compartment to the nucleus, and altered protein levels of Bcl-2, Bcl-xL and Bax. Furthermore, resveratrol promoted H446 cell inhibition by cisplatin, as reflected by reduced viability and increased apoptosis. These findings suggest that resveratrol exerts antitumor effects on SCLC H446 cells and promotes H446 cell killing by cisplatin via modulation of intrinsic apoptosis.

Resveratrol inhibits viability and induces apoptosis in the small‑cell lung cancer H446 cell line via the PI3K/Akt/c‑Myc pathway

“…The cells were randomly divided into a blank control group, Res group, Res+ N-acetyl-L-cysteine (NAC) group, and NAC group. The treatment concentrations of Res and NAC [a reactive oxygen species (ROS) inhibitor] were 40 µg/ml and 2 mmol/l, respectively, in line with our previous research results (7,8). Cells in the Res+NAC group were first treated with 2 mmol/l of NAC for 2 h and then with 40 µg/ml Res for 24 h.…”

“…In our previous research, the relationship between the concentration of Res and toxicity in H446 cells was explored, confirming that Res has potent toxic effects on the SCLC H446 cell line ( 7 ). Based on these findings, 40 µg/ml Res was used in the present study to further explore the anticancer effects of Res in H446 cells.…”

“…A number of studies have found that Res has potential anticancer activity in tumors of multiple systems in the human body ( 6 , 11 ), although there are few studies on the anticancer effects of Res in lung cancer. Previous research by our group revealed that Res concentration- and time-dependently decreased the viability of H446 cells, promoted their apoptosis through the mitochondrial apoptosis pathway, and boosted the antitumor effect of cisplatin on H446 cells ( 7 ).…”

“…Zeng et al (6) revealed that the anticancer activity of Res affects the entire process of cancer development and can inhibit the proliferation of cancer cells and induce their apoptosis. Our previous research results (7) revealed that Res significantly inhibited the viability of H446 SCLC cells and had a synergistic effect on cisplatin, indicating that their combination could enhance the anticancer effects of cisplatin on H446 cells.…”

Resveratrol synergizes with cisplatin in antineoplastic effects against AGS gastric cancer cells by inducing endoplasmic reticulum stress‑mediated apoptosis and G2/M phase arrest

“…RES, a natural polyphenol compound found in plants, is considered to have potent anticancer activity against various types of cancers ( 12 – 15 , 27 ). Moreover, RES has been reported to act as a chemical sensitizer and to enhance the anticancer effect of DDP in prostate cancer ( 29 ), lung cancer ( 30 , 31 ) and hepatoma ( 32 ). Herein, it was revealed that RES exerted antineoplastic effects against the GC cell line AGS by suppressing cell viability and promoting cell apoptosis in a dose-dependent manner.…”