Resveratrol Prevents Warm Ischemia–Reperfusion Injury in Liver Grafts From Non–Heart-Beating Donor Rats

Shimizu, Kenji; Miyagi, Shigehito; Miyazawa, Kohtaro; Maida, Kai; Kashiwadate, Toshiaki; Hara, Yasuyuki; Goto, Masafumi; Kawagishi, Naoki; Ohuchi, Noriaki

doi:10.1016/j.transproceed.2015.11.031

Cited by 7 publications

(9 citation statements)

References 24 publications

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“…Resveratrol (3,5,4'-trihydroxystilbene, RSV) is a non-flavonoid polyphenol found mainly in grapes. It has pleiotropic effects, including anti-cancer, anti-aging, anti-inflammatory, and antioxidant actions, as well as cardioprotective and neuroprotective properties (Shimizu et al, 2016). The effects of RSV during the freezing of sperm have been studied in recent work.…”

Section: Introductionmentioning

confidence: 99%

Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing

Zhai

Duan

et al. 2020

J. Zhejiang Univ. Sci. B

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Resveratrol (3,5,4'-trihydroxystilbene, RSV) has been widely used in mammalian cells, but whether it can be used during freezing boar semen is still unknown. The effects of RSV treatment during boar semen freezing on its anti-freezing ability, apoptosis, and possible apoptotic pathways were observed in this study. Sperm motility, mitochondrial membrane potential (∆Ψ m), adenosine triphosphate (ATP) content, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL)-positive apoptotic state, and messenger RNA (mRNA) expression levels of apoptotic genes involved in different apoptotic pathways after freezing with or without RSV treatment were tested. The results showed that: (1) Compared with fresh sperm, the motility, normal acrosome rate, and plasma membrane integrity rate of frozen boar sperm decreased significantly (P<0.05), and RSV did not significantly increase the sperm motility (0.44 vs. 0.40, P>0.05), but it did significantly improve the normal acrosome rate (57.65% vs. 47.00%, P<0.05) and plasma membrane integrity rate (46.67% vs. 38.85%, P<0.05). (2) After freezing, most boar sperm showed low mitochondrial ∆Ψ m. RSV treatment could increase the rate of high mitochondrial ∆Ψ m of boar sperm. (3) RSV treatment significantly decreased reactive oxygen species (ROS) levels (58.65% vs. 88.41%, P<0.05) and increased the ATP content (0.49 μmol/L vs. 0.25 μmol/L, P<0.05) of boar sperm during freezing. (4) The apoptotic rate of the freezing group (80.41%) with TUNEL detection increased significantly compared to the fresh group (9.70%, P<0.05), and RSV treatment greatly decreased the apoptotic rate (68.32%, P<0.05). (5) Real-time polymerase chain reaction (RT-PCR) showed that not only the genes from the death receptor-mediated apoptotic pathway (tumor necrosis factor-α (TNF-α), Fas ligand (FasL), and Caspase-8), but also the genes from the mitochondria-mediated apoptotic pathway (manganese superoxide dismutase (MnSOD), B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and Caspase-9) were both significantly changed after freezing. RSV treatment during freezing greatly changed their expression levels. Although RSV treatment during boar semen freezing did not significantly increase motility after thawing, it still played an efficient antioxidant role, which could enhance the mitochondrial function and decrease the apoptotic level induced by both the death receptor-and mitochondria-mediated apoptotic pathways.

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Section: Introductionmentioning

confidence: 99%

Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing

Zhai

Duan

et al. 2020

J. Zhejiang Univ. Sci. B

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“…The use of organs from cadavers with cardiac arrest (NHBD) to cope with this obstacle is equivocal. Based on the constraint in organ procurement, organ harvest from NHBD has come to the fore several times since the brain death declaration criteria was published in Harvard Medical School in 1986 (7,8). To elucidate the condition in detail, Maastricht (1-4) and Modified Maastricht criteria (1-5) were published (8).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, an urgent organ retrieval is of great importance to protect the organs and minimize the warm ischemia time. Several surgical methods were suggested for this issue (4)(5)(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Urgent Organ Retrieval from Non-Heart-Beating Donor with Declared Brain Death: Harvest at Arrest

Yıldız¹,

Koca²,

Sabuncuoğlu³

2017

chr

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RezumatScop: Din cauza numărului insuficient de donatori disponibili pentru a acoperi nevoia de transplanturi de organe, noi cercetări sunt în desfăşurare. În contextul acesta, cazurile de stop cardiac şi moarte cerebrală au fost evaluate ca donatori probabili în cursul ultimilor ani. Scopul acestui studiu este de a discuta tehnicile salutare de prelevare de organe cu distrugere minimă a ţesutului şi rapiditate maximă de efectuare, în condiţiile asigurate de centrul nostru, precum şi experienţa proprie în acest domeniu. Materiale şi metode: Un număr total de 4 din 13 pacienţi aflaţi în moarte cerebrală care au prezentat stop cardiac în aşteptarea rezultatelor de laborator în cadrul centrului nostru în perioada 2015-2016 au fost conduşi către sala de operaţie de urgenţă sub masaj cardiac şi s-a efectuat prelevare de organe în regim de urgenţă. Datele clinice ale acestui grup specific au fost analizate. Rezultate: În perioada 2015-2016 au existat 13 cazuri de prelevare de organe de la pacienţi aflaţi în moarte cerebrală. Din aceste 13 cazuri, 9 au fost supuse laparotomiei şi canulării de urgenţă, iar organele au fost prelevate după perfuzia cu soluţie rece in situ, fără alte complicaţii. În schimb, în 4 cazuri care au prezentat stop cardiac la care s-a efectuat perfuzie cu soluţii reci ex-vivo din lipsa de mijloace în sala de operaţie, afectarea vasculară şi parenchimală au apărut la nivelul a două organe hepatice şi a 2 rinichi. Utilizând această tehnică au fost prelevate şi transplantate 4 ficati şi 8 rinichi. Concluzii: Laparotomia de urgenţă, canularea şi perfuzia cu soluţii saline reci in situ reprezintă abordarea ideală pentru un timp de

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“…The utilization of resveratrol in animal models had a positive impact on liver preservation. At the functional level this has been reflected by decreased levels of plasma aminotransferase activities [ 219 ], improved bile production [ 220 ] as well as increased portal vein flow volume [ 220 ]. Additionally, attenuation of IRI-induced thioredoxin-interacting protein (Txnip) indicative of liver stress has been reported [ 221 ] along with increased ATP-content of liver grafts [ 220 ].…”

Section: Resveratrolmentioning

confidence: 99%

“…At the functional level this has been reflected by decreased levels of plasma aminotransferase activities [ 219 ], improved bile production [ 220 ] as well as increased portal vein flow volume [ 220 ]. Additionally, attenuation of IRI-induced thioredoxin-interacting protein (Txnip) indicative of liver stress has been reported [ 221 ] along with increased ATP-content of liver grafts [ 220 ]. Moreover, resveratrol treatment induced positive effects on a morphological level including preservation of sinusoidal endothelial cells [ 220 ] along with generally reduced histological liver injury [ 219 ].…”

Section: Resveratrolmentioning

confidence: 99%

Combating Ischemia-Reperfusion Injury with Micronutrients and Natural Compounds during Solid Organ Transplantation: Data of Clinical Trials and Lessons of Preclinical Findings

Mauerhofer

Grumet

Schemmer

et al. 2021

IJMS

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Although extended donor criteria grafts bear a higher risk of complications such as graft dysfunction, the exceeding demand requires to extent the pool of potential donors. The risk of complications is highly associated with ischemia-reperfusion injury, a condition characterized by high loads of oxidative stress exceeding antioxidative defense mechanisms. The antioxidative properties, along with other beneficial effects like anti-inflammatory, antiapoptotic or antiarrhythmic effects of several micronutrients and natural compounds, have recently emerged increasing research interest resulting in various preclinical and clinical studies. Preclinical studies reported about ameliorated oxidative stress and inflammatory status, resulting in improved graft survival. Although the majority of clinical studies confirmed these results, reporting about improved recovery and superior organ function, others failed to do so. Yet, only a limited number of micronutrients and natural compounds have been investigated in a (large) clinical trial. Despite some ambiguous clinical results and modest clinical data availability, the vast majority of convincing animal and in vitro data, along with low cost and easy availability, encourage the conductance of future clinical trials. These should implement insights gained from animal data.

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Resveratrol Prevents Warm Ischemia–Reperfusion Injury in Liver Grafts From Non–Heart-Beating Donor Rats

Cited by 7 publications

References 24 publications

Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing

Effect of resveratrol treatment on apoptosis and apoptotic pathways during boar semen freezing

Urgent Organ Retrieval from Non-Heart-Beating Donor with Declared Brain Death: Harvest at Arrest

Combating Ischemia-Reperfusion Injury with Micronutrients and Natural Compounds during Solid Organ Transplantation: Data of Clinical Trials and Lessons of Preclinical Findings

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