Resveratrol prevents TNF-α-induced muscle atrophy via regulation of Akt/mTOR/FoxO1 signaling in C2C12 myotubes

Wang, Dongtao; Yin, Yi; Yang, Yajun; Lv, Pei-Jia; Shi, Ying; Lu, Lu; Wei, Lianbo

doi:10.1016/j.intimp.2014.02.002

Cited by 140 publications

(116 citation statements)

References 52 publications

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“…Resveratrol has been shown to prevent TNF-α induced muscle atrophy (Wang et al, 2014) and to alleviate oxidative stress also in old mice (Jackson et al, 2011). Therefore, we investigated whether resveratrol attenuates the age-related muscle wasting and normalizes muscle hypertrophy in old mice.…”

Section: Resveratrol Decreases the Sc Density After Overloadmentioning

confidence: 99%

Blunted hypertrophic response in old mouse muscle is associated with a lower satellite cell density and is not alleviated by resveratrol

Ballak

Jaspers

Deldicque

et al. 2015

Experimental Gerontology

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Background: Sarcopenia contributes to the decreased quality of life in the older person.While resistance exercise is an effective measure to increase muscle mass and strength, the hypertrophic response may be blunted in old age.Objectives: To determine 1) whether hypertrophy in the m. plantaris of old mice was blunted compared to adult and 2) whether this was related to a reduced satellite cell (SC) density and 3) how resveratrol affects hypertrophy in old mice. Methods:In adult (7.5 months, n=11), old (23.5 months, n=10) and old-resveratrol-treated (n=10) male C57BL/6j mice, hypertrophy of the left m. plantaris was induced by denervation of its synergists. The contralateral leg served as control.Results: After six weeks, overload-induced myofiber hypertrophy and IIB-IIA shift in myofiber type composition were less pronounced in old than adult mice (P=0.03), irrespective of resveratrol treatment. Muscles from old mice had a lower SC density than adult muscles (P=0.002). Overload-induced SC proliferation (P<0.05) resulted in an increased SC density in old, but not adult muscles (P=0.02), while a decrease occurred after resveratrol supplementation (P=0.044). Id2 and myogenin protein expression levels were higher in old than adult muscles (P<0.05). Caspase-3 was expressed more in hypertrophied than control muscles and was reduced with resveratrol (P<0.05). Conclusion:The blunted hypertrophic response in old mice was associated with a lower SC density, but there was no evidence for a lower capacity for proliferation. Resveratrol did not rescue the hypertrophic response and even reduced, rather than increased, the number of SCs in hypertrophied muscles.

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Section: Resveratrol Decreases the Sc Density After Overloadmentioning

confidence: 99%

Blunted hypertrophic response in old mouse muscle is associated with a lower satellite cell density and is not alleviated by resveratrol

Ballak

Jaspers

Deldicque

et al. 2015

Experimental Gerontology

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“…The phosphorylation of AKT and FoxO is known to be regulated by PI3K (4,41). Therefore, the present study investigated the effects of LY294002, an inhibitor of PI3K.…”

Section: Rg1 Stimulates Pi3k-dependent Phosphorylation Of Akt Andmentioning

confidence: 99%

“…It is associated with a number of human diseases and catabolic conditions, including fasting, disuse, aging, cancer, neuromuscular diseases, stroke, chronic obstructive pulmonary disease, chronic heart failure, HIV-acquired immunodeficiency syndrome and sepsis (1)(2)(3)(4)(5). Muscle volume shrinking and muscle weakness induced by muscular dystrophy typically disrupt and adversely affect the life of patients (3,(6)(7)(8).…”

Section: Introductionmentioning

confidence: 99%

Ginsenoside Rg1 prevents starvation-induced muscle protein degradation via regulation of AKT/mTOR/FoxO signaling in C2C12 myotubes

Peng

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Skeletal muscle atrophy is often caused by catabolic conditions including fasting, disuse, aging and chronic diseases, such as chronic obstructive pulmonary disease. Atrophy occurs when the protein degradation rate exceeds the rate of protein synthesis. Therefore, maintaining a balance between the synthesis and degradation of protein in muscle cells is a major way to prevent skeletal muscle atrophy. Ginsenoside Rg1 (Rg1) is a primary active ingredient in Panax ginseng, which is considered to be one of the most valuable herbs in traditional Chinese medicine. In the current study, Rg1 was observed to inhibit the expression of MuRF-1 and atrogin-1 in C2C12 muscle cells in a starvation model. Rg1 also activated the phosphorylation of mammalian target of rapamycin (mTOR), protein kinase B (AKT), and forkhead transcription factor O, subtypes 1 and 3a. This phosphorylation was inhibited by LY294002, a phosphatidylinositol 3-kinase inhibitor. These data suggest that Rg1 may participate in the regulation of the balance between protein synthesis and degradation, and that the function of Rg1 is associated with the AKT/mTOR/FoxO signaling pathway. IntroductionMuscle atrophy is characterized by an increase in protein degradation and reduction in protein synthesis. It is associated with a number of human diseases and catabolic conditions, including fasting, disuse, aging, cancer, neuromuscular diseases, stroke, chronic obstructive pulmonary disease, chronic heart failure, HIV-acquired immunodeficiency syndrome and sepsis (1-5). Muscle volume shrinking and muscle weakness induced by muscular dystrophy typically disrupt and adversely affect the life of patients (3,6-8). Therefore, it is essential to understand the mechanism by which skeletal muscle atrophy is regulated.A large amount of protein hydrolysis has previously been identified to occur during muscle atrophy and the ubiquitin-proteasome system (UPS) has been demonstrated to be involved in this process (9)(10)(11)(12)(13)(14). In this system, the proteins are first conjugated into multiple molecules of ubiquitin. The 26S proteasome then recognizes and degrades the ubiquitinated proteins (15,16). Multiple enzymes regulate the protein ubiquitination; these include E1, the ubiquitin-activating enzyme, E2, the ubiquitin conjugating enzyme and E3 ubiquitin ligases (17,18). E3 ubiquitin ligases serve a major role in the specificity of protein degradation, because the specific binding between the protein substrate and E3 occurs prior to the reaction with ubiquitin (18,19).It has been demonstrated that the insulin-signaling pathway is involved in the inhibition of UPS (20). In this pathway, the phosphorylation of insulin receptor substrate is stimulated when insulin binds its receptor. Phosphatidylinositol 3-kinase (PI3K), an intracellular intermediate, is recruited to phosphorylate a serine/threonine kinase, protein kinase B (AKT) during this process. AKT then phosphorylates the forkhead box class O transcription factors, subtype 1 and 3a (FoxO1 and FoxO3a), which pre...

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“…Other in vitro studies also demonstrated that resveratrol is capable of reversing muscle mass loss induced by inflammation, mainly the pro-inflammatory cytokine TNF-␣, through regulation of Akt/mTOR/FoxO1 signaling pathway, which enhances MPS required for muscle structure and function. Akt (protein kinase B) is a kinase capable of controlling both MPS, via phosphorylating and activating of mTOR, and MPD, by inactivating of transcription factor Forkhead Box O1 [97]. In addition, intra-gastric administration of resveratrol with a dose of 100 mg/kg/day to 8 month-old rats for 12 weeks decreased ROS level in subsarcolemmal and intermyofibrillar mitochondria and increased antioxidative capacity as measured by total superoxide dismutase (SOD), glutathione peroxidase and catalase enzymes activity levels in tibialis anterior muscle [98].…”

Section: Antioxidantsmentioning

confidence: 99%

Nutritional supplementations and administration considerations for sarcopenia in older adults

Farshidfar

Shulgina

Myrie

2016

NUA

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Abstract. Sarcopenia is a progressive degenerative disorder affecting ≥40% of older adults over the age of 70 years. It is characterized by involuntary muscle loss leading to functional disability, weakness, and frailty in the elderly. Numerous mechanisms have been suggested as potential contributors to sarcopenia onset, including anorexia of aging, protein imbalances, and oxidative stress. While consensus preventive and management approaches for sarcopenia are not yet clearly defined, it is unquestionable that nutrition plays a critical role. This review focuses on the more widely studied, potent nutrients used to attenuate sarcopenia: protein/amino acids, vitamin D and calcium, antioxidants and omega-3 fatty acids. In efforts to achieve optimum delivery of these nutrients to target tissues in older adults it is important to also consider the issue of compromised digestive system that occurs with aging, which will contribute to suboptimal nutrients absorption and utilization. Therefore, this review aims to summarize key sarcopenia-attenuating nutrients and to distinguish for each identified nutrient whether a dietary or a supplemental form is the most effective therapy for nutrient delivery in sarcopenic older adults.

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Resveratrol prevents TNF-α-induced muscle atrophy via regulation of Akt/mTOR/FoxO1 signaling in C2C12 myotubes

Cited by 140 publications

References 52 publications

Blunted hypertrophic response in old mouse muscle is associated with a lower satellite cell density and is not alleviated by resveratrol

Blunted hypertrophic response in old mouse muscle is associated with a lower satellite cell density and is not alleviated by resveratrol

Ginsenoside Rg1 prevents starvation-induced muscle protein degradation via regulation of AKT/mTOR/FoxO signaling in C2C12 myotubes

Nutritional supplementations and administration considerations for sarcopenia in older adults

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