Resveratrol-loaded solid lipid nanoparticles versus nanostructured lipid carriers: evaluation of antioxidant potential for dermal applications

Abstract

Background: Excessive generation of radical oxygen species (ROS) is a contributor to skin pathologies. Resveratrol (RSV) is a potent antioxidant. Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) can ensure close contact and increase the amount of drug absorbed into the skin. In this study, RSV was loaded into SLN and NLC for dermal applications. Methods: Nanoparticles were prepared by high shear homogenization using Compritol 888ATO, Myglyol, Poloxamer188, and Tween80. Particle size (PS), polydispersity index (PI), zeta potential (ZP), drug entrapment efficiency (EE), and production yield were determined. Differential scanning calorimetry (DSC) analysis and morphological transmission electron microscopy (TEM) examination were conducted. RSV concentration was optimized with cytotoxicity studies, and net intracellular accumulation of ROS was monitored with cytofluorimetry. The amount of RSV was determined from different layers of rat abdominal skin. Results: PS of uniform RSV-SLN and RSV-NLC were determined as 287.2 nm ± 5.1 and 110.5 nm ± 1.3, respectively. ZP was −15.3 mV ± 0.4 and −13.8 mV ± 0.1 in the same order. The drug EE was 18% higher in NLC systems. TEM studies showed that the drug in the shell model was relevant for SLN, and that the melting point of the lipid in NLC was slightly lower. Concentrations below 50 µM were determined as suitable RSV concentrations for both SLN and NLC in cell culture studies. RSV-NLC showed less fluorescence, indicating less ROS production in cytofluorometric studies. Ex vivo skin studies revealed that NLC are more efficient in carrying RSV to the epidermis. Conclusion: This study suggests that both of the lipid nanoparticles had antioxidant properties at a concentration of 50 µM. When the two systems were compared, NLC penetrated deeper into the skin. RSV-loaded NLC with smaller PS and higher drug loading appears to be superior to SLN for dermal applications.

The Impact of Variables on Particle Size of Solid Lipid Nanoparticles and Nanostructured Lipid Carriers; A Comparative Literature Review

“…Although it seems that there are no statistical differences in polydispersity indices (PDI) between NLC with 5% (PDI 0.280) and 15% (PDI 0.259) oil and the SLN formulation (PDI 0.263), the NLC formulations with 30% (PDI 0.224) and 45% (PDI 0.213) oil showed smaller size distribution than SLN. 32 The comparison of 6 SLN and NLC formulations loaded with different amounts of sildenafil citrate and prepared with identical processes revealed that NLC showed a lower particle size than SLN in all formulations. This observation was attributed to the better emulsification of the solid lipid matrix when oil is incorporated in the solid matrix.…”

Anti-Aging Cosmetics Approaches and Components in Products

“…Membran lipitlerini ve çekirdek DNA'sını oksidatif hasara karşı belirgin ölçüde korur 61 . [80][81][82][83][84][85][86][87][88] . Sentetik ve doğal kökenli kozmetik bileşenleri açısından yeni taşıyıcı sistemlerin kullanımının birçok avantajı vardır.…”

“…Sentetik ve doğal kökenli kozmetik bileşenleri açısından yeni taşıyıcı sistemlerin kullanımının birçok avantajı vardır. Bitkisel kökenli bileşenlerin biyoparçalanma ve düşük toksisite gibi bazen avantajlı oldukları durumlar da göz önüne alınarak bitkisel bileşenlerin kozmetik formülasyonlarda kullanımında lipozom, fitozom, transferzom, nanoemülsiyon, nanopartikül, mikroemülsiyon, nanokristal, kübozomlar ve mikrosüngerler ile çalışılmıştır [80][81][82][83][84][85][86][87][88][89][90] . Özellikle nanoemülsiyonlar son yıllarda kişisel bakım ürünlerinde sürekli etkili kozmetikler ve aktif bileşenlerin belli deri katmanlarına dağılmasında tercih edilmektedir.…”

“…yaşlanma karşıtı aktif bileşenlerin kozmetik formüllerinde kullanılmasında tercih edilmektedir. Veziküler sistemler kullanılarak deriden su kaybı engellenebilmekte, onarıcı etki ile birlikte vitaminlerin stabilitesinin korunması da sağlanmış olmaktadır [79][80][81][82][83][84][85][86][87][88][89][90][91][92][93] . Mikrokapsüller ve mikroküreler ile güneşten koruyucu aktif maddeler enkapsüle edilebilmiş, vitamin A palmitat krem formülasyonu haline getirilebilmiştir.…”

Preparation of resveratrol-loaded nanoporous silica materials with different structures

“…It is extremely photosensitive compound with low chemical stability, which limits its beneficial therapeutic effects [4]. Thus, loading of resveratrol in nanoporous silica systems can stabilize and protect it from degradation.…”