Resveratrol-loaded polymeric micelles protect cells from Aβ-induced oxidative stress

Lu, Xiaowei; Ji, Chenbo; Xu, Huae; Li, Xiaolin; Ding, Hu; Ye, Min; Zhu, Zhenshu; Ding, Dan; Jiang, Xiqun; Xin-sheng, Ding; Guo, Xirong

doi:10.1016/j.ijpharm.2009.03.021

Cited by 172 publications

(84 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These globular species obtained from our experimental conditions were similar to those observed by other groups [59][60][61][62] and were reported to be nontoxic to PC12 neuronal cells. 63,64 TEM results were in agreement with the collected EIS and fluorescence data.…”

Section: Thioflavin T Assay Of Aβsupporting

confidence: 86%

Electrochemical Detection of Amyloid-Beta Aggregation in the Presence of Resveratrol

Hung

Cheng

et al. 2013

J. Electrochem. Soc.

View full text Add to dashboard Cite

The aggregation of amyloid-β (Aβ) is an important pathologic feature of Alzheimer's disease (AD) that leads to the formation of neuronal plaques. A rapidly growing number of polyphenols have been intensively investigated for AD treatment. In this report, the interaction of a polyphenolic compound, resveratrol, with Aβ was studied using electrochemical impedance spectroscopy (EIS). The growth of Aβ fibrils was facilitated with the incubation of oligomer-modified electrodes with free Aβ in solution. Resveratrol significantly slowed down the formation of Aβ aggregates, which was monitored by following the changes in the charge transfer resistance (R ct ) values. The moderating effect of resveratrol on Aβ aggregation was also confirmed with Thioflavin T (ThT) fluorescence assay and transmission electron microscopy (TEM) imaging. Results obtained from EIS correlated well with the ThT fluorescence assay and suggested that EIS was a promising method for the analysis of Aβ aggregation in the presence of novel Aβ-targeting compounds toward AD therapy. Various factors contribute to the deterioration of health in the aging population. Of those, dementia is a prominent worldwide issue that is currently under intense investigation in an effort to discover novel therapeutic agents to slow the progression of this disease. Alzheimer's disease (AD) has been found to be the most common cause of dementia, in which a multitude of regulatory pathways are affected. As the disease progresses, physical manifestations include reduced mobility, changes in behavior and a decline in cognitive function.

show abstract

Section: Thioflavin T Assay Of Aβsupporting

confidence: 86%

Electrochemical Detection of Amyloid-Beta Aggregation in the Presence of Resveratrol

Hung

Cheng

et al. 2013

J. Electrochem. Soc.

View full text Add to dashboard Cite

show abstract

“…Albumin nanoparticles constitute a promising drug carrier system for the transport of drugs and for specific target delivery [83][84][85][86]. Other incorporations of RSV into nanoparticles show a higher cell death compared with an equivalent dose of free RSV and an enhancement of RSV stability [87][88][89]. In these experiments, lipophilic RSV could be incorporated into the hydrophobic core of the nanosphere, while its hydrophilic outer shell still exists as a stabilizer for the system.…”

Section: Impact Of Rsv Transport On Its Bioavailability and Its Biolomentioning

confidence: 99%

Role of membrane dynamics processes and exogenous molecules in cellular resveratrol uptake: Consequences in bioavailability and activities

Delmas

Lin

2011

Molecular Nutrition Food Res

View full text Add to dashboard Cite

In the fields of nutrition prevention and therapy treatment, numerous studies have reported interesting properties of trans-resveratrol (RSV), a natural polyphenol against pathologies such as vascular diseases, cancers, viral infections and neurodegenerative processes. These beneficial effects are supported by more studies showing the pleiotropic actions of RSV. Nevertheless, a crucial question concerning these effects is how the polyphenol, when applied to an organism, gains access to its targets. In this review, we focus on the biochemical and biological parameters involved in RSV transport, particularly the role of the phospholipid bilayer in RSV uptake (passive diffusion, carrier-mediated transport) and of exogenous molecules modulating RSV transport and effects. The dynamic processes of the plasma membrane reveal the importance of the role of lipid composition in the fluidity, the lipid rafts in RSV endocytosis and the ATP-binding cassette transporters in RSV efflux. Specific membrane receptors such as integrin αvβ3 contribute to RSV uptake and to activate signalling pathways involved in apoptosis. We discuss the role of intracellular receptors (i.e. aryl-hydrocarbon and estrogen receptors). In addition, circulating molecules (i.e. albumin, haemoglobin, fatty acids, lipoproteins) play a role as RSV carriers. Finally, we developed a hypothesis concerning the relation between RSV uptake and its biological activities.

show abstract

“…[1][2][3] However, in most studies resveratrol has been used in its free form, which is not suitable for drug delivery. Nanocarriers have rarely been considered, [4][5][6][7][8][9] thus presenting a challenge still to be pursued. The development of site-specific drug delivery systems that protect resveratrol during its transit inside the organism is extremely important to preserve its pharmacological properties, while enhancing its bioavailability after oral administration.…”

Section: Introductionmentioning

confidence: 99%

Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability

Neves¹,

Lúcio²,

Martins³

et al. 2013

IJN

133

View full text Add to dashboard Cite

Introduction:Resveratrol is a polyphenol found in grapes and red wines. Interest in this polyphenol has increased due to its pharmacological cardio-and neuroprotective, chemopreventive, and antiaging effects, among others. Nevertheless, its pharmacokinetic properties are less favorable, since the compound has poor bioavailability, low water solubility, and is chemically unstable. To overcome these problems, we developed two novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance resveratrol's oral bioavailability for further use in medicines, supplements, and nutraceuticals. Methods and materials: Solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) loaded with resveratrol were successfully produced by a modified hot homogenization technique. These were completely characterized to evaluate the quality of the developed resveratrol-loaded nanoparticles. Results: Cryo-scanning electron microscopy morphology studies showed spherical and uniform nanoparticles with a smooth surface. An average resveratrol entrapment efficiency of ∼70% was obtained for both SLNs and NLCs. Dynamic light scattering measurements gave a Z-average of 150-250 nm, polydispersity index of ∼0.2, and a highly negative zeta potential of around −30 mV with no statistically significant differences in the presence of resveratrol. These characteristics remained unchanged for at least 2 months, suggesting good stability. Differential scanning calorimetry studies confirmed the solid state of the SLNs and NLCs at both room and body temperatures. The NLCs had a less ordered crystalline structure conferred by the inclusion of the liquid lipid, since they had lower values for phase transition temperature, melting enthalpy, and the recrystallization index. The presence of resveratrol induced a disorder in the crystal structure of the nanoparticles, suggesting a favoring of its entrapment. The in vitro release studies on conditions of storage showed a negligible resveratrol release over several hours for both nanosystems and the in vitro simulation of gastrointestinal transit showed that the resveratrol remained mostly associated with the lipid nanoparticles after their incubation in digestive fluids. Conclusion: Both nanodelivery systems can be considered suitable carriers for oral administration, conferring protection to the incorporated resveratrol and allowing a controlled release after uptake.

show abstract

Resveratrol-loaded polymeric micelles protect cells from Aβ-induced oxidative stress

Cited by 172 publications

References 36 publications

Electrochemical Detection of Amyloid-Beta Aggregation in the Presence of Resveratrol

Electrochemical Detection of Amyloid-Beta Aggregation in the Presence of Resveratrol

Role of membrane dynamics processes and exogenous molecules in cellular resveratrol uptake: Consequences in bioavailability and activities

Novel resveratrol nanodelivery systems based on lipid nanoparticles to enhance its oral bioavailability

Contact Info

Product

Resources

About