Resveratrol-Loaded Lipid Nanocarriers: Correlation between In Vitro Occlusion Factor and In Vivo Skin Hydrating Effect

Abstract

Lipid nanocarriers show occlusive properties that may be related to their ability to improve skin hydration. The aim of this work was to evaluate the relationship between in vitro occlusion factor and in vivo skin hydration for three types of lipid nanocarriers: nanoemulsions (NEs), solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs). These lipid nanocarriers were loaded with trans-resveratrol (RSV) and incorporated in gel vehicles. In vitro occlusion factor was in the order SLNs > NLCs > NEs. Gels containing unloaded or RSV loaded lipid nanocarriers were applied on the back of a hand of 12 healthy volunteers twice a day for one week, recording skin hydration changes using the instrument Soft Plus. An increase of skin hydration was observed for all lipid nanocarriers (SLNs > NLCs > NEs). RSV loading into these nanocarriers did not affect in vitro and in vivo lipid nanocarriers effects. A linear relationship (r2 = 0.969) was observed between occlusion factor and in vivo increase of skin hydration. Therefore, the results of this study showed the feasibility of using the occlusion factor to predict in vivo skin hydration resulting from topical application of different lipid nanocarriers loading an active ingredient with no inherent hydrating activity.

Development and Characterization of Nanoemulsion Containing Almond Oil, Biodegradable Polymer and Propranolol as Potential Treatment in Hemangioma

“…These results are in agreement with the study of Montenegro et al (2017) where it was observed that the gels containing non‐crystalline nanocarriers without solid lipid, e.g. gel with nanoemulsion, showed an occlusion factor significantly higher that the control gel, suggesting that the liquid lipid may contribute to the occlusive properties of the nanocarriers …”

“…An ideal drug delivery system fulfills the objective of maximizing therapeutic effect while minimizing toxicity . Nanoemulsion (NE) is a drug delivery system constituted by nanodroplets with a size between 20 and 200 nm surrounded by surfactant.…”

“…These systems have many advantages compared to conventional emulsions. Usually, NE can exist as oil‐in‐water (O/W) but can also be water‐in‐oil (W/O) or multiple emulsions W/O/W . Their very large interfacial area positively influences the drug transport and their delivery to target specific sites.…”

In Vitro Antioxidant Activity and In Vivo Topical Efficacy of Lipid Nanoparticles Co-Loading Idebenone and Tocopheryl Acetate

“…In vivo topical efficacy under in use conditions of unloaded and IDE-loaded SLN and NLC was assessed according to a protocol previously described [11,12,28]. Fourteen female subjects with average photo-aging were selected (age range: 45-63 years) and experiments were performed according to the rules of the Declaration of Helsinki of 1975, revised in 2008.…”

“…In the last decades, solid lipid nanoparticles (SLNs) have been widely investigated as carriers for topical drug delivery [1][2][3][4][5][6][7]. These colloidal systems, consisting of a solid lipid matrix stabilized by different types of surfactants, have been proposed for skin delivery of various active ingredients including antifungal, anticancer, anti-inflammatory, and antioxidant agents [8][9][10][11][12][13][14][15]. The interest in SLNs arises from their many advantages compared to other colloidal carriers such as possibility of incorporating both hydrophilic and lipophilic drugs, ability to achieve drug-controlled release and targeting, thus improving drug topical bioavailability while reducing systemic side effects [1,4].…”