Resveratrol inhibits phosphorylation within the signal transduction and activator of transcription 3 signaling pathway by activating sirtuin 1 in SW1353 chondrosarcoma cells

Abstract

The present study assessed the mechanism by which resveratrol (Res) inhibits the growth of SW1353 chondrosarcoma cells and examined whether sirtuin 1 (Sirt1) activation affects phosphorylation within the signal transduction and activator of transcription 3 (STAT3) signaling pathway. The present study used SW1353 chondrosarcoma cells in the logarithmic phase of growth (control and treatment groups). The latter group was treated with Res at 25 and 50 µmol/l for 24 h, and cell viability, proliferation and apoptosis were analyzed using the cell counting kit‑8 assay, colony counting and Hoechst staining, respectively. The expression levels of caspase‑3, cleaved caspase‑3, B‑cell lymphoma‑2 (BCL‑2), BCL-2 associated X protein (Bax), STAT3 and phosphorylated (p‑)STAT3) were measured by Western blotting. SW1353 cells were transfected with small interfering (si)RNA targeting Sirt1 and the expression levels of Sirt1, STAT3 and p-STAT3 were assessed. Exposure of SW1353 cells to Res reduced cell viability in a dose‑dependent manner (P<0.01). Additionally, cell proliferation was significantly inhibited and the cell nuclei exhibited apoptotic characteristics. Cleaved caspase‑3, Sirt1 and Bax levels were upregulated. The expression levels of BCL‑2 and p‑STAT3 were downregulated. Additionally, the BCL‑2/Bax ratio was reduced compared with the control group. The total STAT3 level was unaffected. Res treatment activated Sirt1, however, in cells transfected with Sirt1‑siRNA, the ability of resveratrol to suppress p‑STAT3 expression was compromised. Overall, it was revealed that Res treatment induced apoptosis, inhibited proliferation and affected phosphorylation within the STAT3 signaling pathway by activating Sirt1 in SW1353 chondrosarcoma cells.

Induction of sirtuin-1 signaling by resveratrol induces human chondrosarcoma cell apoptosis and exhibits antitumor activity

“…Jin et al . indicated that treatment with SIRT1 agonist resveratrol in vitro induced apoptosis, inhibited proliferation, and affected phosphorylation within the STAT3 signaling pathway by activating Sirt1 in SW1353 chondrosarcoma cells 47 . However, the role of SIRT1 in oncogenic and tumor-suppressive function in chondrosaroma still remains to be clarified.…”

High expression of PIWIL2 promotes tumor cell proliferation, migration and predicts a poor prognosis in glioma

“…PIWIL2 has already been demonstrated to inhibit cell apoptosis through the activation of the Stat3/Bcl-XL pathway (34). Similar to Bcl-XL, Bcl-2 and caspase 3 are also regulated by STAT3, which leads to cell apoptosis (35)(36)(37). In our study, we determined that downregulated expression of PIWIL2 led to the increased expression of cleaved caspase 3 while it inhibited Bcl-2 expression, which meant that PIWIL2 may inhibit glioma cell apoptosis by suppressing nuclear to mitochondrial translocation as well as the subsequent caspase activation.…”

Therapeutic Targets and Emerging Treatments in Advanced Chondrosarcoma

“…Resveratrol, a dietary phytochemical found in various plant species, has shown antitumor effects on several types of malignant tumors [81]. Jin et al investigated the effect of resveratrol on tumor growth and sirtuin 1 activity in chondrosarcoma cells [82]. They reported that resveratrol showed antitumor effects, including the inhibition of cell viability and proliferation, the induction of apoptosis, and the suppression of phosphorylation within the STAT3 signaling pathway, in chondrosarcoma cells.…”