Resveratrol inhibits nitric oxide and TNF-α production by lipopolysaccharide-activated microglia

Bi, Xiu Li; Yang, Jing; Dong, Ying; Wang, Ji Ming; Cui, Yong Hong; Ikeshima, Takashi; Zhao, Yu; Wu, Chun Fu

doi:10.1016/j.intimp.2004.08.008

Cited by 255 publications

(173 citation statements)

References 52 publications

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“…These results coincide or agree with those of other research groups. Indeed, a similar finding has recently been reported and thus nature molecules such as flavonoids and tocopherols inhibit the release of proinflammatory cytokines after activating microglia with LPS [11][12][13].…”

Section: Anti-inflammatory Activity Of Gp-ee On Lps-activated N13 Cellssupporting

confidence: 82%

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Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Rodríguez-Morgado¹,

Candiracci

Santa-María³

et al. 2014

Plant Foods Hum Nutr

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Grape pomace, a winemaking industry by-product, is a rich source of bioactive dietary compounds. Using proteases we have developed an enzymatic process for obtaining a water-soluble extract (GP-EE) that contains biomolecules such as peptides, carbohydrates, lipids and polyphenols in soluble form. Of especial interest is its high polyphenol content (12 %), of which 77 % are flavonoids and 33 % are phenolic acids. The present study evaluates in vitro the potential anti-inflammatory effect of GP-EE by monitoring the expression of inflammatory molecules on N13 microglia cells stimulated with lipopolysaccharide (LPS). GP-EE decreases the mRNA levels of the inflammatory molecules studied. The molecules under study were as follows: inducible nitric oxide synthase (iNOS), tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), the ionized calcium binding adaptor molecule-1(Iba-1) and the Toll like receptor-4 (TLR-4), as well as the iNOS protein level in LPS-stimulated microglia. Our findings suggest that, as a result of its ability to regulate excessive microglial activation, GP-EE possesses antiinflammatory properties. Therefore, acting as a chemopreventive agent, it may be of therapeutic interest in neurodegenerative diseases involving neuroinflammation. We can, therefore, propose GP-EE as a useful natural extract and one that would be beneficial to apply in the field of functional foods.

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Section: Anti-inflammatory Activity Of Gp-ee On Lps-activated N13 Cellssupporting

confidence: 82%

“…It would be interesting, therefore, to search for molecules that could help control inflammation in the CNS [7][8][9][10]. Natural polyphenols have been shown to exert neuroprotective properties by inhibiting the release of proinflammatory cytokines after LPS-activation of microglia [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Rodríguez-Morgado¹,

Candiracci

Santa-María³

et al. 2014

Plant Foods Hum Nutr

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“…Resveratrol has been found to modulate neuroinflammation in both in vivo and in vitro models, specifically targeting activated micloglial cells [71]. Actually, resveratrol suppresses the activation of the NF-kB pathway in LPS-activated microglia by reducing the phosphorylation and consequent degradation of its inhibitor, IkB [72][73][74][75]. Our results demonstrate that microglial, but not astroglial reactivity, was significantly diminished with resveratrol administration compared to untreated SOD1 G93A mice.…”

Section: Discussionmentioning

confidence: 60%

Resveratrol Improves Motoneuron Function and Extends Survival in SOD1G93A ALS Mice

et al. 2014

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Amyotrophic lateral sclerosis (ALS) is an adult onset neurodegenerative disease that causes progressive paralysis and death due to degeneration of motoneurons in spinal cord, brainstem and motor cortex. Nowadays, there is no effective therapy and patients die 2-5 years after diagnosis. Resveratrol (trans-3,4′,5-trihydroxystilbene) is a natural polyphenol found in grapes, with promising neuroprotective effects since it induces expression and activation of several neuroprotective pathways involving Sirtuin1 and AMPK. The objective of this work was to assess the effect of resveratrol administration on SOD1 G93A ALS mice. We determined the onset of symptoms by rotarod test and evaluated upper and lower motoneuron function using electrophysiological tests.We assessed the survival of the animals and determined the number of spinal motoneurons. Finally, we further investigated resveratrol mechanism of action by means of western blot and immunohistochemical analysis. Resveratrol treatment from 8 weeks of age significantly delayed disease onset and preserved lower and upper motoneuron function in female and male animals. Moreover, resveratrol significantly extended SOD1 G93A mice lifespan and promoted survival of spinal motoneurons. Delayed resveratrol administration from 12 weeks of age also improved spinal motoneuron function preservation and survival. Further experiments revealed that resveratrol protective effects were associated with increased expression and activation of Sirtuin 1 and AMPK in the ventral spinal cord. Both mediators promoted normalization of the autophagic flux and, more importantly, increased mitochondrial biogenesis in the SOD1 G93A spinal cord. Taken together, our findings suggest that resveratrol may represent a promising therapy for ALS.

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“…RV (5 mg/kg bw for 30 days) found a reduction of the levels of blood glucose, glycosylated hemoglobin, TNF-alpha, interleukin-1-beta, interleukin-6, NF-kappaB p65 unit and NO with increase in plasma insulin in diabetic rats (29). Bi et al (30) found that RV treatment significantly inhibited the release of TNF-alpha and nitrite oxide induced by lipopolysaccharide (LPS) in rat cortical microglia and a mouse microglial cell line N9 in vitro. RV inhibited the degradation of IkappaB alpha, expression of iNOS and phosphorylation of p38 mitogen-activated protein kinases in N9 microglial cells treated with LPS.…”

Section: Discussionmentioning

confidence: 97%

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

Aydın¹,

Şahin²,

Bacanlı³

et al. 2016

Balkan Med J

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Background:The increases of free radicals have been proposed to be involved in the pathogenesis of sepsis, which leads to multiple-organ dysfunction syndromes. The uses of antioxidants as a complementary tool in the medical care of oxidative stress-related diseases have attracted attention of researchers. Resveratrol (RV) has suggested being antioxidant, anti-proliferative, and anti-inflammatory effects in various experimental models and clinical settings. Aims: This study was undertaken to evaluate the protective effects of RV on oxidative DNA damage induced by sepsis in the liver and kidney tissues of Wistar albino rats. Study Design: Animal experimentation. Methods: Four experimental groups consisting of eight animals for each was created using a total of thirty-two male Wistar albino rats. Sham group was given 0.5 mL of saline intra-peritoneal (ip) only following laparatomy. Sepsis group was given 0.5 mL saline ip only following the induction of sepsis. RV-treated group was given a dose of 100 mg/kg ip RV in 0.5 mL saline following laparatomy. RVtreated sepsis group was given 100 mg/kg ip RV in 0.5 mL saline following the induction of sepsis. A model of sepsis was created by cecal ligation and puncture technique. In the liver and kidney tissues, oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX)) and a proinflammatory cytokine (tumor necrosis factor alpha (TNF-alpha)), were evaluated spectrophotometrically and DNA damage was determined by the alkaline single cell gel electrophoresis (comet assay) technique using formamidopyrimidine DNA glycosylase protein. Results:In the RV-treated sepsis group, the levels of MDA and TNF-alpha were lower and GSH levels, SOD and GPX activities were higher than in the septic rats (p<0.05). RV treatment significantly reduced the sepsis-induced oxidative DNA damage in the liver and kidney cells (p<0.05). Conclusion: It is suggested that RV treatment might reduce the sepsis-induced oxidative DNA damages in sepsis-related diseases; however, there is a need for more studies to clear up the protective mechanisms of RV against sepsis.

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Resveratrol inhibits nitric oxide and TNF-α production by lipopolysaccharide-activated microglia

Cited by 255 publications

References 52 publications

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Obtaining from Grape Pomace an Enzymatic Extract with Anti-inflammatory Properties

Resveratrol Improves Motoneuron Function and Extends Survival in SOD1G93A ALS Mice

Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats

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