Resveratrol inhibits cell growth in a human cholangiocarcinoma cell line

Roncoroni, Leda; Elli, Luca; Dolfini, E.; Erba, Eugenio; Dogliotti, Elena; Terrani, C.; Doneda, Luisa; Grimoldi, Maria Grazia; Bardella, Maria Teresa

doi:10.1111/j.1478-3231.2008.01749.x

Cited by 31 publications

(33 citation statements)

References 31 publications

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“…Significantly reduced inflammatory cells in NDMA+TUR at 2 months was observed, as well as formation of new bile ducts. This was correlated with increased ALP levels, which may be produced from bile duct epithelial cells (Roncoroni et al 2008). The pathological changes from O. viverrini infection alone were observed from the inflammatory cells (mononuclear cells and eosinophils) surrounding the hepatic bile ducts, as well as epithelial hyperplasia, goblet cell metaplasia, adenomatous metaplasia, and thickened periductal fibrosis (Fig.…”

Section: Discussionmentioning

confidence: 88%

Turmeric reduces inflammatory cells in hamster opisthorchiasis

et al. 2009

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The curcumin compound from turmeric is effective in the treatment of many inflammatory diseases. The aim of our present study was to evaluate the efficacy of turmeric on reducing the histopathological changes of hamster opisthorchiasis. Hamsters were infected with Opisthorchis viverrini and then administered turmeric. Using light microscopic observation, liver function tests for alanine transaminase (ALT), alkaline phosphatase, and direct bilirubin were investigated. The resulting histopathological changes show that turmeric has anti-inflammatory properties--during both N-nitrosodimethylamine administration and O. viverrini infection--by reducing the aggregation of inflammatory cells surrounding the hepatic bile ducts, which correlates with a decreased serum ALT level. The decrease in direct bilirubin levels in the hamsters treated with turmeric suggests that turmeric may enhance biliary contraction. The present study found that turmeric clearly reduces the inflammatory cells in hamster opisthorchiasis at an early stage. This finding may be connected with a reduction in the risk factors of cholangiocarcinoma development.

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Section: Discussionmentioning

confidence: 88%

Turmeric reduces inflammatory cells in hamster opisthorchiasis

et al. 2009

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“…The inhibitory effects of RESV on cell cycle have been previously demonstrated, as has its ability to mediate tumour cell death selectively through necrosis, apoptosis, autophagy and other mechanisms, although evidence is emerging that certain normal cells such as endothelial cells, lymphocytes, chondrocytes and adipocytes are vulnerable to RESV [27][28][29][30]. Likewise, RESV inhibits proliferation and promotes apoptosis of several tumour lines, such as osteosarcoma, epidermoid carcinoma, macroglobulinemia malignant cells or breast cancer cells [15,16,18,20]. Furthermore, in the majority of studies, RESV inhibits cell proliferation and induces apoptosis via different mechanisms [29,30].…”

Section: Discussionmentioning

confidence: 95%

“…as a cofactor [12,13]. Since the discovery of the involvement of SIRT 1 in apoptosis, cell survival, transcription, metabolism and aging, the interests in SIRT1 physiological effects has increased [12,[14][15][16]. Current research is therefore focused to understand the mechanisms involved in the ability of RESV to increase the activity of SIRT1 and on the intracellular pathways regulated by this protein.…”

Section: Introductionmentioning

confidence: 98%

Resveratrol Inhibits Proliferation and Promotes Apoptosis of Neuroblastoma Cells: Role of Sirtuin 1

et al. 2010

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Resveratrol prolongs lifespan and prevent cancer formation; however, the mechanisms are not understood. Here we evaluated the cell-cycle inhibition and apoptosis of resveratrol in B65 neuroblastoma cells, and we also studied the effects of resveratrol on the mammalian silent information regulator 2 (SIRT1). Results show that resveratrol reduces cell viability and causes apoptosis at 24 h of treatment. Resveratrol partially blocked cell proliferation, and significantly increased the fraction of cells arrested in the S phase. The role of SIRT1 in cell-cycle effects mediated by resveratrol was studied through changes in the expression of SIRT1 using western blot. Exposure to resveratrol decreased SIRT1 content, concomitant with an increase in the acetylated form of sirtuin substrates p53 and NFκ-β. Treatment of B65 neuroblastoma cells with resveratrol also reduced the content of the phosphorylated form of AKT. Exposure to the SIRT1 inhibitors nicotinamide and sirtinol altered neither cell viability nor the fraction of apoptotic cells. Furthermore, when cells were exposed simultaneously to resveratrol and nicotinamide or sirtinol, no changes were observed in the fraction of apoptotic cells. Our results show that a decrease in SIRT1 content, caused by exposure to resveratrol, does not appear to be involved in cell-cycle arrest or activation of apoptosis.

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“…Optical density was measured at 450 nm with microplates reader (Bouty diagnostici, Milan, Italy). The results were normalized referring to tissue weight [13]. Caspase 9 was evaluated colorimetrically by means of instant ELISA technique following the manufacturer's instructions (Bender, Milano, Italy), as the transforming growth factor b (TGFb) evaluation (Bendey).…”

Section: Chemoluminescent Multiparametric Cytokine Array and Analysismentioning

confidence: 99%