Resveratrol inhibit interleukin-1β induced inflammatory response in human osteoarthritis chondrocytes through NF-κB signaling pathway

Abstract

Osteoarthritis (OA) is a common degenerative disease and affect millions of people. Resveratrol is a coumarin compound refined from traditional Chinese medicines with potential anti-inflammatory ability. This study aimed to evaluate protective anti-inflammatory effects of resveratrol in human OA chondrocytes. The chondrocytes were isolated from OA patients. CCK-8 method was used to explore the optimal dose of resveratrol for chondrocyte. Next, we used PCR and Western-blot method to assess the relative mRNA and protein expression in IL-1β group. We further explored relevant mechanism of resveratrol for anti-inflammatory in human OA chondrocytes by immunofluorescence and Western-blot. The results showed that resveratrol blocked IL-1β-stimulated production of NO and PGE2. In addition, resveratrol inhibited the expression of COX-2, iNOs, MMP-1, MMP-3, MMP-13, and increased the levels of aggrecan and collagen-II. Mechanistically, resveratrol suppressed IL-1βinduced IκB-α degradation and NF-κB activation. In conclusion, our results demonstrate that resveratrol inhibits inflammation in OA via the regulation of NF-κB signaling pathway, and suggested that resveratrol may be a potential therapeutic agent for OA. BackgroundOsteoarthritis (OA) acts as a prevalent chronic age-related disease joint disease featuring cartilage degradation, osteophyte formation, and joint stiffness, which seriously affects the health of the elderly people [1] . There is an increase in the incidence rate of OA with the aging of population, thus it is necessary to find an effective strategy for the treatment of OA [2] .During the progression of OA, the increased expression of interleukin-1β (IL-1β) in the articular cartilage and synovium leads to the destruction of cartilage, downregulation of type II collagen [3] .Furthermore, IL-1β has the function of inducing the chondrocyte release of prostaglandin E2 (PGE2) and nitric oxide (NO). Thus, reducing the IL-1β in articular cavity could delay the development of OA [4] .Resveratrol is a common polyphenolic compound in the skin of red grapes [5] . Resveratrol exhibits anti-ageing, anti-inflammatory, and anti-oxidant activities, and has cardio-, neuro-, and chondroprotective effects as proved by many studies [6] . Nuclear factor-kappa B (NF-κB) signaling XFS, YC and QRD conducted experiments; MQZ analyzed data; QRD and XFS wrote manuscript; YC revised manuscript; XFS and YC designed the experiment and edited manuscript.

Advancing Wound Healing: Innovative Pathway Strategies for Enhanced Recovery

“…Moreover, LncRNA XIST targets miR-29b-3p/COL1A1¹⁶ and can also inhibit miR-29a and promote LIN28A expression, e ectively contributing to the synthesis of HSFs¹⁴ ,17 . Finally, XIST is also crucial in alleviating pain behavior as it suppresses cytokines that cause in ammation 14,18 . These results suggest that LncRNA XIST may be a promising therapeutic target for promoting wound healing.…”