Resveratrol influences pulmonary mechanics and inflammatory response in a porcine ARDS model

Rissel, René; Kirchner, Louisa; Renz, Miriam; Mohnke, Katja; Riedel, Julian; Ruemmler, Robert; Hartmann, Erik K.; Kamuf, Jens; Ziebart, Alexander

doi:10.1016/j.lfs.2023.121410

“…RSV acts on different biological targets and thus produces different biological effects, therefore, it is being considered as a potential drug for the treatment of vascular diseases 17 , neurodegenerative diseases 18 , in ammatory disorders 19 , atherosclerosis, and cancer 20,21 . According to the latest reports, RSV has a bene cial in uence on the coronavirus disease (COVID-19) 22 .…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling to evaluate the impact of amantadine and rimantadine on the secondary metabolism of a model organism

Kostina-Bednarz

¹

,

Płonka

²

,

Barchańska

³

2023

Preprint

0

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Metabolic profiling offers huge potential to highlight markers and mechanisms in support of toxicology and pathology investigations during drug development. The main objective was to modify therapy with adamantane derivatives: amantadine and rimantadine, to increase their bioavailability and evaluate the influence of such therapy on drug metabolism using Saccharomyces cerevisiae as the model organism. In this study, the profile of endogenous metabolites of a model organism was measured and interpreted to provide an opportunity to investigate changes induced by treatment with amantadine and rimantadine. It was found that resveratrol supplementation synergistically enhanced the effects of amantadine treatment and increased rimantadine metabolism, potentially reducing side effects. The fingerprinting strategy was used as an efficient technique for qualitatively evaluating and monitoring changes in the profiles of endogenous components and their contents in a model organism. Chemometric tools were employed to find marker compounds that can be defined as characteristic indicators of a pharmacological response to a therapeutic intervention. An improved understanding of the mechanisms involved in drug effect and an increased ability to predict individual variations in the drug response of organisms will improve the treatment process and the development of new therapies.

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“…RSV acts on different biological targets and thus produces different biological effects, therefore, it is being considered as a potential drug for the treatment of vascular diseases 17 , neurodegenerative diseases 18 , inflammatory disorders 19 , atherosclerosis, and cancer 20 , 21 . According to the latest reports, RSV has a beneficial influence on the coronavirus disease (COVID-19) 22 .…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling to evaluate the impact of amantadine and rimantadine on the secondary metabolism of a model organism

Kostina-Bednarz,

Płonka,

Barchanska

2023

Sci Rep

1

0

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Metabolic profiling offers huge potential to highlight markers and mechanisms in support of toxicology and pathology investigations during drug development. The main objective was to modify therapy with adamantane derivatives: amantadine and rimantadine, to increase their bioavailability and evaluate the influence of such therapy on drug metabolism using Saccharomyces cerevisiae as the model organism. In this study, the profile of endogenous metabolites of a model organism was measured and interpreted to provide an opportunity to investigate changes induced by treatment with amantadine and rimantadine. It was found that resveratrol supplementation synergistically enhanced the effects of amantadine treatment and increased rimantadine metabolism, potentially reducing side effects. The fingerprinting strategy was used as an efficient technique for qualitatively evaluating and monitoring changes in the profiles of endogenous components and their contents in a model organism. Chemometric tools were employed to find marker compounds that can be defined as characteristic indicators of a pharmacological response to a therapeutic intervention. An improved understanding of the mechanisms involved in drug effect and an increased ability to predict individual variations in the drug response of organisms will improve the treatment process and the development of new therapies.

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Effects of volume management on free flap perfusion and metabolism in a large animal model study

Thiem,

Stephan,

Ziebart

et al. 2024

Lab Anim

0

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Free flap failure represents a substantial clinical burden. The role of intraoperative volume management remains controversial, with valid studies lacking. Here, using a large animal model, we investigated the influence of volume management on free flap perfusion and metabolism. Autotransfer of a musculocutaneous gracilis flap was performed on 31 German domestic pigs, with arterial anastomosis and catheterization of the pedicle vein for sequential blood sampling. Flap reperfusion was followed by induction of a hemorrhagic shock with maintenance for 30 min and subsequent circulation stabilization with crystalloid solution, crystalloid solution and catecholamine, autotransfusion or colloidal solution. Flap perfusion and oxygenation were periodically assessed using hyperspectral imaging. Flap metabolism was assessed via periodic blood gas analyses. Hyperspectral imaging revealed no difference in either superficial or deep tissue oxygen saturation, tissue hemoglobin or tissue water content between the test groups at any time point. Blood gas analyses showed that lactate levels were significantly increased in the group that received crystalloid solution and catecholamine, after circulatory stabilization and up to 2 h after. We conclude that, in hemorrhagic shock, volume management impacts acid–base balance in free flaps. Crystalloid solutions with norepinephrine increase lactate levels, yet short-term effects on flap perfusion seem minimal, suggesting that vasopressors are not detrimental.

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Resveratrol influences pulmonary mechanics and inflammatory response in a porcine ARDS model

Cited by 4 publications

References 25 publications

Metabolic profiling to evaluate the impact of amantadine and rimantadine on the secondary metabolism of a model organism

Metabolic profiling to evaluate the impact of amantadine and rimantadine on the secondary metabolism of a model organism

Metabolic profiling to evaluate the impact of amantadine and rimantadine on the secondary metabolism of a model organism

Effects of volume management on free flap perfusion and metabolism in a large animal model study

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