Resveratrol induces SIRT1-Dependent autophagy to prevent H2O2-Induced oxidative stress and apoptosis in HTR8/SVneo cells

Wang, Ping; Huang, Chenxi; Gao, Jun; Shi, Ying; Li, Hong; Yan, Huan; Yan, Shujun; Zhang, Zhan

doi:10.1016/j.placenta.2020.01.002

Cited by 41 publications

(25 citation statements)

References 43 publications

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“…Araloside A and L-ascorbic acid exerted synergistic antioxidant effects, probably due to the combination of the glycosylation activity of araloside A and the dehydrogenation reaction of ascorbic acid. [36]. In contrast, the maximum oxidative damage was achieved when Chinese hamster cells were incubated with high-dose H 2 O 2 for 5 min [37].…”

Section: Discussionmentioning

confidence: 91%

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H₂O₂‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Tian

Zhang

et al. 2021

Oxidative Medicine and Cellular Longevity

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Araloside A is a pentacyclic triterpenoid saponin, and L-ascorbic acid is a globally recognized antioxidant. In this study, coadministered araloside A and L-ascorbic acid were found to have a strong synergistic antioxidant effect, and correlations between cellular antioxidant indexes and free radical scavenging ability were found. Individual and combined pretreatment with araloside A and L-ascorbic acid increased both cell viability and antioxidant enzyme activity and inhibited the release of lactate dehydrogenase (LDH); the accumulation of malondialdehyde (MDA), lipid peroxidation (LPO) products, and H2O2; and the production of intracellular reactive oxygen species (ROS), protein carbonyls, and 8-hydroxy-2-deoxy guanosine (8-OHdG). Free radical scavenging ability was positively correlated with superoxide dismutase (SOD) and catalase (CAT) activity, the glutathione (GSH)/oxidized glutathione (GSSG) ratio, and total antioxidant capacity (T-AOC). Our study is the first investigation of araloside A and L-ascorbic acid coadministration for the treatment of diseases caused by oxidative stress. The synergistic antioxidant effects of araloside A and L-ascorbic acid support their potential as functional food ingredients for the elimination of oxidative stress-induced adverse reactions.

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Section: Discussionmentioning

confidence: 91%

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H₂O₂‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Tian

Zhang

et al. 2021

Oxidative Medicine and Cellular Longevity

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“…Indeed, some studies that focused on the use of antioxidants, such as vitamins C and E, were not successful [36,37]. However, resveratrol, a potent natural antioxidant and SIRT1 activator presented mainly in grapes and wine, emerges as a possible supporter to attenuate PE symptoms as it has been shown by our group [38] and others [33,[39][40][41][42]. Nevertheless, more research on the side effects of the use of SIRT1 activators in humans is necessary given previous reports of its tissuedependent activity [43,44].…”

Section: Discussionmentioning

confidence: 96%

Analysis of SIRT1 Expression in Plasma and in an In Vitro Model of Preeclampsia

Viana-Mattioli

Nunes

Cavalli

et al. 2020

Oxidative Medicine and Cellular Longevity

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Preeclampsia (PE) is a pregnancy-specific disorder that affects 3–8% expecting mothers worldwide being one of the main causes of maternal and fetal morbidity and mortality. The search for altered circulating molecules in PE is an important target to better understand the pathophysiology of this disease. Therefore, we evaluated Sirtuin-1 (SIRT1) concentration in plasma from healthy pregnant (HP) women, gestational hypertensive women (GH), and preeclampsia women (PE) via enzyme-linked immunosorbent assay (ELISA). We also measured intracellular SIRT1 in HUVECs incubated with plasma from PE patients compared to HP and GH via Western Blot Assay. Statistical differences were considered when p<0.05. SIRT1 was downregulated in PE compared to HP and GH, both in plasma and in in vitro assay. Similarly, SIRT1 was also reduced in pregnant women who subsequently developed PE (case) compared to women who had healthy pregnancies (control). This reduction may be indicative of possible underlying pathophysiology mechanisms in PE.

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“…The modulation of autophagy by resveratrol has been intensively investigated in the last decades [150,[304][305][306][307][308]. In the recent study, Gong and Xia showed that treatment melanoma cells with resveratrol led to the upregulation of proteins associated with autophagy (Beclin 1 and microtubule-associated protein 1A/1B-light chain 3 (LC3)-II/I), while the p62 expression was downregulated [309].…”

Section: Modulation Of Autophagy and Apoptosis By Polyphenolsmentioning

confidence: 99%

Polyphenols as Caloric-Restriction Mimetics and Autophagy Inducers in Aging Research

et al. 2020

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It has been thought that caloric restriction favors longevity and healthy aging where autophagy plays a vital role. However, autophagy decreases during aging and that can lead to the development of aging-associated diseases such as cancer, diabetes, neurodegeneration, etc. It was shown that autophagy can be induced by mechanical or chemical stress. In this regard, various pharmacological compounds were proposed, including natural polyphenols. Apart from the ability to induce autophagy, polyphenols, such as resveratrol, are capable of modulating the expression of pro- and anti-apoptotic factors, neutralizing free radical species, affecting mitochondrial functions, chelating redox-active transition metal ions, and preventing protein aggregation. Moreover, polyphenols have advantages compared to chemical inducers of autophagy due to their intrinsic natural bio-compatibility and safety. In this context, polyphenols can be considered as a potential therapeutic tool for healthy aging either as a part of a diet or as separate compounds (supplements). This review discusses the epigenetic aspect and the underlying molecular mechanism of polyphenols as an anti-aging remedy. In addition, the recent advances of studies on NAD-dependent deacetylase sirtuin-1 (SIRT1) regulation of autophagy, the role of senescence-associated secretory phenotype (SASP) in cells senescence and their regulation by polyphenols have been highlighted as well. Apart from that, the review also revised the latest information on how polyphenols can help to improve mitochondrial function and modulate apoptosis (programmed cell death).

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Resveratrol induces SIRT1-Dependent autophagy to prevent H2O2-Induced oxidative stress and apoptosis in HTR8/SVneo cells

Cited by 41 publications

References 43 publications

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H₂O₂‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H₂O₂‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Analysis of SIRT1 Expression in Plasma and in an In Vitro Model of Preeclampsia

Polyphenols as Caloric-Restriction Mimetics and Autophagy Inducers in Aging Research

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Resveratrol induces SIRT1-Dependent autophagy to prevent H2O2-Induced oxidative stress and apoptosis in HTR8/SVneo cells

Cited by 41 publications

References 43 publications

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H2O2‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H2O2‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Analysis of SIRT1 Expression in Plasma and in an In Vitro Model of Preeclampsia

Polyphenols as Caloric-Restriction Mimetics and Autophagy Inducers in Aging Research

Contact Info

Product

Resources

About

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H₂O₂‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status

Synergistic Antioxidant Effects of Araloside A and L‐Ascorbic Acid on H₂O₂‐Induced HEK293 Cells: Regulation of Cellular Antioxidant Status