Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Iwata, Hisataka

doi:10.1002/rmb2.12401

Cited by 16 publications

(9 citation statements)

References 94 publications

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“…Our data suggested that Res might have counteracted Cd-toxicity by up-regulating SIRT1 contributing to overexpression of the antioxidant genes such as, SOD1 and GPX1. We can also hypothesize that Res might have stimulated SIRT1 mediated mt turnover in Cd-exposed oocytes [63,64]. This aspect requires further investigations.…”

Section: Discussionmentioning

confidence: 91%

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Piras

Ariu

Maltana

et al. 2022

J Animal Sci Biotechnol

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Background Heavy metal cadmium (Cd) is a widespread environmental contaminant with a potential toxicity that might negatively affect female reproduction and fertility. It has been reported that Cd exposure impaired the quality of oocytes and led to a defective maturation and fertilization, through oxidative stress induction. Resveratrol (Res) is a natural polyphenol with strong antioxidant properties that exhibited protective role in preventing oocyte redox homeostasis disruption and quality decline. Here, we explored whether the addition of Res to in vitro maturation (IVM) medium might act as a protection against Cd-induced toxicity on ovine oocyte maturation and fertilization. Firstly, we evaluated the effect of supplementing IVM medium with two different Res concentrations (1 and 2 μmol/L) on nuclear maturation and fertilization of oocytes matured under CdCl2 (2 μmol/L) exposure. Therefore, the concentration of 1 μmol/L Res was selected to analyse the effects of this compound on intracellular ROS levels, mitochondrial (mt) distribution and activity, chromatin configuration, cytoskeleton morphology, cortical granules (CGs) distribution and mRNA expression of genes associated with cellular response to oxidative stress (i.e. SIRT1, SOD 1, GPX1, GSR, CAT) in Cd-exposed in vitro matured oocytes. Results We found that 1 μmol/L Res restored the reduced oocyte meiotic competence induced by Cd exposure as well as, Res sustained oocyte ability to be normally fertilized and decreased polyspermic fertilization at both tested concentrations. Moreover, we demonstrated that 1 μmol/L Res mitigated Cd-induced alterations of oocyte cytoplasmic maturation by reducing reactive oxygen species (ROS) accumulation, preventing mt dysfunction, maintaining the correct meiotic spindle and cortical F-actin assembly and the normal cortical granule distribution as well as up-regulating SIRT1, SOD1 and GPX1 genes. Conclusions Taken together, our findings highlighted the beneficial influence exerted by Res in preventing Cd-induced disturbance of nuclear and cytoplasmic maturation and subsequent fertilization in ovine oocytes. Res treatment may help to establish defence strategies counteracting Cd-induced toxicity on the female gamete.

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Section: Discussionmentioning

confidence: 91%

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Piras

Ariu

Maltana

et al. 2022

J Animal Sci Biotechnol

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“…Mitochondria are the essential organelles involved in many cellular functions and have a pivotal role in oocyte competence and embryo development (42). Vitrification of oocytes or embryos has been reported to cause mitochondrial dysfunction such as influencing distribution, mitochondrial ROS production, membrane potential, ATP content and others (43,44). For the vitrified porcine zygotes, although mitochondrial activity showed a decrease in 4-cell embryos, it was completely restored at the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

Astaxanthin Supplementation Improves the Subsequent Developmental Competence of Vitrified Porcine Zygotes

et al. 2022

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Cryopreservation of embryos has been confirmed to cause oxidative stress as a factor responsible for impaired developmental competence. Currently, astaxanthin (Ax) raises considerable interest as a strong exogenous antioxidant and for its potential in reproductive biology. The present study aimed to investigate the beneficial effects of Ax supplementation during in vitro culture of vitrified porcine zygotes and the possible underlying mechanisms. First, the parthenogenetic zygotes were submitted to vitrification and then cultured in the medium added with various concentrations of Ax (0, 0.5, 1.5, and 2.5 μM). Supplementation of 1.5 μM Ax achieved the highest blastocyst yield and was considered as the optimal concentration. This concentration also improved the blastocyst formation rate of vitrified cloned zygotes. Moreover, the vitrified parthenogenetic zygotes cultured with Ax exhibited significantly increased mRNA expression of CDX2, SOD2, and GPX4 in their blastocysts. We further analyzed oxidative stress, mitochondrial and lysosomal function in the 4-cell embryos and blastocysts derived from parthenogenetic zygotes. For the 4-cell embryos, vitrification disturbed the levels of reactive oxygen species (ROS) and glutathione (GSH), and the activities of mitochondria, lysosome and cathepsin B, and Ax supplementation could fully or partially rescue these values. The blastocysts obtained from vitrified zygotes showed significantly reduced ATP content and elevated cathepsin B activity, which also was recovered by Ax supplementation. There were no significant differences in other parameters mentioned above for the resultant blastocysts. Furthermore, the addition of Ax significantly enhanced mitochondrial activity and reduced lysosomal activity in resultant blastocysts. In conclusion, these findings revealed that Ax supplementation during the culture period improved subsequent embryonic development and quality of porcine zygotes after vitrification and might be used to ameliorate the recovery culture condition for vitrified embryos.

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“…Mihanfar et al found that quercetin, also an agonist of SIRT1, was involved in regulating energy homeostasis via upregulating the expression of SIRT1 and AMPK, with beneficial effects on insulin resistance and hormonal indices [ 42 ]. Hisataka found that resveratrol increased SIRT1 expression levels and enhanced mitochondrial recovery from cryopreservation-induced damage in oocytes and embryos [ 43 ]. Itami et al also found that resveratrol increased the level of ATP synthesis and improved the quality of porcine oocytes in early sinus follicles through the activation of SIRT1 during sow oocyte development [ 44 ].…”

Section: Regulation Of Oxidative Stress and Mitochondrial Function By...mentioning

confidence: 99%

Regulation of SIRT1 in Ovarian Function: PCOS Treatment

et al. 2023

CIMB

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The sirtuin family, a group of NAD+-dependent class 3 histone deacetylases (HDACs), was extensively studied initially as a group of longevity genes that are activated in caloric restriction and act in concert with nicotinamide adenine dinucleotides to extend the lifespan. Subsequent studies have found that sirtuins are involved in various physiological processes, including cell proliferation, apoptosis, cell cycle progression, and insulin signaling, and they have been extensively studied as cancer genes. In recent years, it has been found that caloric restriction increases ovarian reserves, suggesting that sirtuins may play a regulatory role in reproductive capacity, and interest in the sirtuin family has continued to increase. The purpose of this paper is to summarize the existing studies and analyze the role and mechanism of SIRT1, a member of the sirtuin family, in regulating ovarian function. Research and review on the positive regulation of SIRT1 in ovarian function and its therapeutic effect on PCOS syndrome.

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Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Cited by 16 publications

References 94 publications

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Protective effect of resveratrol against cadmium-induced toxicity on ovine oocyte in vitro maturation and fertilization

Astaxanthin Supplementation Improves the Subsequent Developmental Competence of Vitrified Porcine Zygotes

Regulation of SIRT1 in Ovarian Function: PCOS Treatment

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