Resveratrol Decreases the Invasion Potential of Gastric Cancer Cells

Abstract

The cancer-preventive agent Resveratrol (RSV) [3,5,4′-trihydroxytrans-stilbene] is a widely recognized antioxidant molecule with antitumoral potential against several types of cancers, including prostate, hepatic, breast, skin, colorectal, and pancreatic. Herein, we studied the effect of RSV on the cell viability and invasion potential of gastric cancer cells. AGS and MKN45 cells were treated with different doses of RSV (0–200 μM) for 24 h. Cell viability was determined using the Sulphorhodamine B dye (SRB) assay. For invasion assays, gastric cells were pre-treated with RSV (5–25 μM) for 24 h and then seeded in a Transwell chamber with coating Matrigel. The results obtained showed that RSV inhibited invasion potential in both cell lines. Moreover, to elucidate the mechanism implicated in this process, we analyzed the effects of RSV on SOD, heparanase, and NF-κB transcriptional activity. The results indicated that RSV increased SOD activity in a dose-dependent manner. Conversely, RSV significantly reduced the DNA-binding activity of NF-κB and the enzymatic activity of heparanase in similar conditions, which was determined using ELISA-like assays. In summary, these results show that RSV increases SOD activity but decreases NF-kB transcriptional activity and heparanase enzymatic activity, which correlates with the attenuation of invasion potential in gastric cancer cells. To our knowledge, no previous study has described the effect of RSV on heparanase activity. This article proposes that heparanase could be a key effector in the invasive events occurring during gastric cancer metastasis.

Combination therapy of cisplatin and resveratrol to induce cellular aging in gastric cancer cells: Focusing on oxidative stress, and cell cycle arrest

“…Although the exact mechanism of action has not been fully defined yet, resveratrol induced cell senescence, cell cycle arrest, and increased anti-oxidant capacity ( Rojo et al, 2022 ) (decreased the invasion) as well as reducing metastasis in former preclinical studies ( Pyo et al, 2020 ). However, its biological effects might vary considering different cell types ( Farhadnejad et al, 2019 ).…”

“…The promising advantage of resveratrol on preventing AGS cells invasion and metastasis was stated in-vitro , especially at the concentrations of 5–25 µM ( Rojo et al, 2022 ). Almost similarly, the current experiment proved a reduction in cells migration following treatment of AGS cells with cisplatin-resveratrol combination, at all concentrations.…”

Comparative efficacy of five most common traditional Chinese medicine monomers for promoting recovery of motor function in rats with blunt spinal cord injury: a network meta-analysis

“…This pathophysiological status is alleviated by RSV owing to its ability to relieve inflammation, inhibit oxidative damage, and prevent apoptosis. Furthermore, previous studies have demonstrated that RSV suppresses the inflammatory response in SCI by upregulating the SIRT-1 signaling pathway and downregulating the NF-κB signaling pathways ( 36 , 37 ). SCI is associated with the generation of free radicals, which cause oxidative degradation of lipids ( 38 ).…”

Inhibitory Potential of Resveratrol in Cancer Metastasis: From Biology to Therapy

“…Additionally, Rojo et al have demonstrated that RES administered at doses ranging from 5 to 25 µM can enhance superoxide dismutase (SOD) activity, while concurrently suppressing NF-κB transcriptional activity and heparanase activity in gastric cancer cells (AGS and MKN45). This finding suggests that RES may have a role in reducing the invasive potential of gastric cancer cells [66]. Furthermore, Buhrmann et al reported that RES can downregulate TNF-β-induced colorectal cancer cell (HCT116) proliferation by blocking TNF-β/TNF-β-receptor-induced NF-κB activation, resulting in activation of caspase-3 cleavage [67].…”