Resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in rolC transgenic cell cultures of Vitis amurensis

Dubrovina, Alexandra S.; Manyakhin, A. Y.; Zhuravlev, Yuri N.; Kiselev, Konstantin V.

doi:10.1007/s00253-010-2792-z

Cited by 62 publications

(38 citation statements)

References 30 publications

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“…Similar results were reported for Rubia cordifolia cells, in which the rolB gene was more effective than the rolC gene, in the activation of antraquinone biosynthesis (Bulgakov et al 2002). In V. amurensis calli, the rolB gene was also shown to be more effective in the stimulation of resveratrol biosynthesis than the rolC gene, as the rolB gene insertion led to a more than 100-fold increase in resveratrol production, whereas the rolC gene caused a 4-to 12-fold increase in the resveratrol accumulation (Kiselev et al 2007;Dubrovina et al 2010). …”

Section: Productivity Of the Mb1 Mb2 And Mb4 Callus Culturesmentioning

confidence: 99%

RolB gene-induced production of isoflavonoids in transformed Maackia amurensis cells

Grishchenko

Kiselev

Tchernoded

et al. 2016

Appl Microbiol Biotechnol

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Maackia amurensis Rupr. et Maxim is a valuable leguminous tree grown in the Russian Far East, in China, and in Korea. Polyphenols from the heartwood of this species (primarily stilbenes and isoflavonoids) possess strong hepatoprotective activity. Callus culture of M. amurensis produced isoflavonoids and their derivatives. In pharmacological experiments, the callus complex was at least as effective, as the plant complex. To increase the yield of isoflavonoids, calli were transformed with the rolB gene of Agrobacterium rhizogenes. Neomycin phosphotransferase (nptII) gene was used for transgenic cell selection. Three rolB transgenic callus lines with different levels of the rolB gene expression were established. Insertion of the rolB gene caused alterations in callus structure, growth, and isoflavonoid production, and stronger alterations were observed with higher expression levels. MB1, MB2, and MB4 cultures accumulated 1.4, 1.5, and 2.1 % of dry weight (DW) isoflavonoids, respectively. In contrast, the empty vector-transformed MV culture accumulated 1.22 % DW. Isoflavonoid productivity of the obtained MB1, MB2, and MB4 cultures was equal to 117, 112, and 199 mg/L of medium, respectively, comparing to 106 mg/L for the MV culture. High level of expression of the rolB gene in MB4 culture led to a 2-fold increase in the isoflavonoid content and productivity and reliably increased dry biomass accumulation. Lower expression levels of the rolB gene in MB1 and MB2 calli did not significantly enhance biomass accumulation and isoflavonoid content, although the rolB gene activated isoflavonoid biosynthesis during the early growth stages and caused the increased content of several distinct compounds.

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Section: Productivity Of the Mb1 Mb2 And Mb4 Callus Culturesmentioning

confidence: 99%

RolB gene-induced production of isoflavonoids in transformed Maackia amurensis cells

Grishchenko

Kiselev

Tchernoded

et al. 2016

Appl Microbiol Biotechnol

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“…Overexpression of the STS genes involved in its biosynthesis can be a promising approach for producing resveratrol. There are consti tutive and inducible forms of STS genes, and their expression is greatly increased with an increasing con tent of resveratrol [14]. It has been suggested previ ously that the enzymatic activity of protein products of constitutive and inducible STS genes may vary [14].…”

Section: Introductionmentioning

confidence: 99%

Influence of overexpression of stilbene synthase VaSTS7 gene on resveratrol production in transgenic cell cultures of grape Vitis amurensis Rupr.

Kiselev

Aleynova

2016

Appl Biochem Microbiol

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We studied reservatrol biosynthesis in cells of the grape V. amurensis with overexpression of the VaSTS7 gene. The present study demonstrated that overexpression of the VaSTS7 gene increased the resver atrol content in transgenic grape cells by 3.2-6.6 times as compared with control cells. Also, the transforma tion enhanced dry biomass accumulation; resveratrol production therefore increased significantly, by 4.7-9.7 times, as compared with control cells.

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“…However, when E. coli received the 4CL gene from Lithospermum erythrorhizon and the STS gene from A. hypogea, and coumaroyl-CoA was used as a substrate, resveratrol production reached 171 mg/L (Katsuyama et al 2007). Significant progress in increasing the resveratrol content in plant cells has been achieved by transforming grape cells with the rolC gene of Agrobacterium rhizogenes (Dubrovina et al 2010) and treating the cell cultures with methyljasmonate and cyclodextrins that resulted in a resveratrol content of 1,600 μmol/g (Lijavetzky et al 2008). In the current study, we found that the resveratrol production by Alternaria sp.…”

Section: Discussionmentioning

confidence: 99%

Alternaria sp. MG1, a resveratrol-producing fungus: isolation, identification, and optimal cultivation conditions for resveratrol production

Shi

Qin

Liu

et al. 2012

Appl Microbiol Biotechnol

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Due to its potential in preventing or slowing the occurrence of many diseases, resveratrol (3,5,4'-trihydroxystilbene) has attracted great research interest. The objective of this study was to identify microorganisms from selected plants that produce resveratrol and to optimize the conditions for resveratrol production. Endophytes from Merlot wine grapes (Vitis vinifera L. cv. Merlot), wild Vitis (Vitis quinquangularis Rehd.), and Japanese knotweed (Polygonum cuspidatum Siebold & Zucc.) were isolated, and their abilities to produce resveratrol were evaluated. A total of 65 isolates were obtained and 21 produced resveratrol (6-123 μg/L) in liquid culture. The resveratrol-producing isolates belonged to seven genera, Botryosphaeria, Penicillium, Cephalosporium, Aspergillus, Geotrichum, Mucor, and Alternaria. The resveratrol-producing capability decreased or was completely lost in most isolates after three rounds of subculture. It was found that only the strain Alternaria sp. MG1 (isolated from cob of Merlot using GA1 medium) had stable and high resveratrol-producing capability in all subcultures. During liquid cultivation of Alternaria sp. MG1 in potato dextrose medium, the synthesis of resveratrol began on the first day, increased to peak levels on day 7, and then decreased sharply thereafter. Cell growth increased during cultivation and reached a stable and high level of biomass after 5 days. The best fermentation conditions for resveratrol production in liquid cultures of Alternaria sp. MG1 were an inoculum size of 6 %, a medium volume of 125 mL in a 250-mL flask, a rotation speed of 101 rpm, and a temperature of 27 °C.

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Resveratrol content and expression of phenylalanine ammonia-lyase and stilbene synthase genes in rolC transgenic cell cultures of Vitis amurensis

Cited by 62 publications

References 30 publications

RolB gene-induced production of isoflavonoids in transformed Maackia amurensis cells

RolB gene-induced production of isoflavonoids in transformed Maackia amurensis cells

Influence of overexpression of stilbene synthase VaSTS7 gene on resveratrol production in transgenic cell cultures of grape Vitis amurensis Rupr.

Alternaria sp. MG1, a resveratrol-producing fungus: isolation, identification, and optimal cultivation conditions for resveratrol production

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