Resveratrol attenuates high-fat diet-induced obesity and the aging-related sarcopenia mitochondrial dysfunction in skeletal muscle

Abstract

Running title: Resveratrol attenuates sarcopenic obesity. Abstract words:243The maximum word count of the main text (including the main text and figure legends, but not the title page, abstract, materials and methods section or reference list): 4222 AbstractSarcopenic obesity is a progressive loss of skeletal muscle mass and strength with increases in adiposity. The aim of this study was to investigate the effects of resveratrol on obesity or sarcopenia to potential therapy risk for skeletal muscle declines in physical function. C57BL/6J male mice were fed either a high-fat diet for 4 weeks and resveratrol (low-, middle-, and high-dose) for 8 weeks to express the obesity effect. Samp8 mice sarcopenia skeletal muscle functional deterioration expressed an age-associated decline. Resveratrol (150 mg/Kg BW, daily) was administered by oral gavage two times a week one month of the experimental period. Exercise training based on adaptations in the muscle is training twice a week for 4 weeks. The skeletal muscles from mice in each group were analyzed by H&E staining, TUNEL and western blot analysis to determine mitochondrial function expression, apoptosis and relative fibrosis signaling. Results of the present study indicate that resveratrol in obesity skeletal muscle is linked to an increase in the expression of mitochondrial function involved in Bcl-2 and PI3K/AKT. On the other hand, resveratrol attenuates sarcopenia Samp8 mice, the age-related loss of skeletal muscle mass and mitochondrial function involved in Bad, caspase 3 and IL-6/ERK1. However, exercise training not find a significant difference in sarcopenia skeletal muscles SAMP8 mice. Exercise training didn't induce sarcopenia skeletal muscle hypertrophy in sarcopenic SAMP8 mice. Therefore, we suggest that resveratrol as a therapeutic potential in the combination of sarcopenia and obesity, the state called sarcopenic obesity.

The beneficial therapeutic effects of plant‐derived natural products for the treatment of sarcopenia

“…Resveratrol attenuated age‐related loss of skeletal muscle mass and mitochondrial function. However, exercise training had no significant effects on sarcopenia in SAMP8 mice 60 …”

“…However, exercise training had no significant effects on sarcopenia in SAMP8 mice. 60 In a different experimental study by Toniolo et al, aged C57/BL6 mice ingested a diet enriched with 0.04% resveratrol for 6 months, while mice that received no treatment were considered the control group. Results showed that mice that ingested the resveratrol-supplemented diet had less tubular aggregates (TAs), which are a morphological feature with increases in densely-arranged tubules from the sarcoplasmic reticulum of striated muscles.…”