Resveratrol and Red Wine Extracts Inhibit The Growth of Caga+ Strains of Helicobacter Pylori in Vitro

Abstract

In 1994, Helicobacter pylori was classified as a group I carcinogen and a definite cause of gastric cancer in humans by the International Agency for Research on Cancer (1). Since then, H. pylori has been epidemiologically linked to adenocarcinoma of the distal stomach (2,3), and a recent study has also found a positive association between H. pylori infection and colorectal adenomas (4). CagA is the strain-specific H. pylori gene that has been linked to the development of premalignant and malignant histological lesions (5). Thus, susceptibility of cagA+ H. pylori strains is of note because, as compared with cagAstrains, infections caused by cagA+ strains significantly increase the risk for developing severe gastric inflammation, atrophic gastritis, and noncardia gastric adenocarcinoma (5). Previously, we have demonstrated that resveratrol, a stilbene from red wine, inhibited the growth of 15 clinical strains of H. pylori in vitro and suggested that the anti-H. pylori activity of resveratrol may play a role in its chemopreventative effects (6). In this investigation, the antibacterial activities of two red wine extracts (Pinot Noir) and resveratrol were assessed against five cagA+ H. pylori strains: accession numbers M23-3, GTD7-13, G1-1, SS1 (Sydney Strain cagA+), and the ATCC 43504 (Rockville, MD) possessing the cagA+ gene and expressing vacuolating cytotoxin. The H. pylori cagA+ strains were obtained from Drs.

Antibacterial Effects of Grape Extracts onHelicobacter pylori

“…Phenolic compounds in grape products play important roles for antioxidant, anti-inflammatory, and antimicrobial effects (13,18,20). In this study, muscadine grape seed extract contained the highest total phenolic content (645.5 mg GAE/g dw), followed by muscadine synergy and skin extracts (381.9 and 135.0 mg GAE/g dw, respectively).…”

“…Grapes (Vitis vinifera), well known for their high levels of antioxidants and polyphenols, have also shown promise as novel antimicrobial agents. A few studies have already reported the anti-H. pylori activities of grape seed and wine, including an active chemical constituent (e.g., resveratrol, a stilbene from red wine) (13). However, no effort has been made to evaluate the grape skin or different grape types (e.g., table and muscadine grapes).…”

Antibiotic treatment forHelicobacter pylori: Is the end coming?

“…Epigallocatechin gallate, one of the green tea catechins, showed significant cytoprotective effects against H. pylori associated gastric cytotoxicity [163] . Red wine and resveratrol have also been shown to inhibit the growth of H. pylori cagA+ strains in vitro [164] . In an openlabel RCT, adding vitamin C and E to antibiotic regimens showed excellent H. pylori eradication rates.…”

In Vitro Activity of Olive Oil Polyphenols against Helicobacter pylori

“…Herbal extracts and essential oils have been used as traditional medicines for thousands of years all over the world, and their anti-H. pylori activity has been widely demonstrated in vitro (2)(3)(4)(5). Many foodstuffs have also exhibited inhibitory activity against the growth of H. pylori in vitro, among others, red wine (6,7), sprouted peas (8), green tea (9), and cranberry juice (10). In many cases, the antibacterial activity of both herbal extracts and foodstuffs has been associated with their content in phenolic compounds, in particular, flavonoids (11)(12)(13), resveratrol (6), and hydrolyzable tannins (14).…”

“…Many foodstuffs have also exhibited inhibitory activity against the growth of H. pylori in vitro, among others, red wine (6,7), sprouted peas (8), green tea (9), and cranberry juice (10). In many cases, the antibacterial activity of both herbal extracts and foodstuffs has been associated with their content in phenolic compounds, in particular, flavonoids (11)(12)(13), resveratrol (6), and hydrolyzable tannins (14). The mechanism by which phenolic compounds affect the growth of H. pylori is unknown, but different theories have been proposed, for example, inhibition of the urease activity (15), adhesion to human gastric mucus (10), disintegration of the outer membrane (16), and inhibition of VacA citotoxin activity which causes the development of inflammation and ulceration in patients (17,18).…”