Resveratrol Ameliorates the Components of Hepatic Inflammation and Apoptosis in a Rat Model of Streptozotocin‐Induced Diabetes

Pektaş, Mehmet Bilgehan; Sadı, Gökhan; Koca, Halit Buğra; Yüksel, Yasemin Topal; Vurmaz, Ayhan; Koca, Tulay; Tosun, Murat

doi:10.1002/ddr.21287

Cited by 18 publications

(11 citation statements)

References 30 publications

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“…These findings proposed that resveratrol may promote hepatic function affecting insulin-signaling pathway in liver, however its action on the pathway may vary between different tissues of genders and models of pathology. There is some evidence in previous studies that inhibition of inflammatory pathways by resveratrol may contribute to alleviation of hepatic injury induced by dietary fructose or streptozotocin treatment [10,25,26]. Here, reduction of hepatic levels of TNFα and IL-1β may account for attenuation of hepatic degeneration and hyperemia in male and female rats that are subjected to fructose feeding.…”

Section: Discussionmentioning

confidence: 96%

Dietary Fructose-Induced Hepatic Injury in Male and Female Rats: Influence of Resveratrol

et al. 2016

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Relatively little is known about gender-dependent susceptibility to hepatic injury induced by nutritional factors. In the current study, we investigated dietary fructose-induced hepatic degeneration and roles of endothelial nitric oxide synthase (eNOS), insulin receptor (IRβ) and substrate-1 (IRS-1) expressions in association with inflammatory markers in male and female rats. Moreover, we examined potential effect of resveratrol on fructose-induced changes. Male and female rats were divided into 4 groups as control, resveratrol, fructose and resveratrol plus fructose. All rats were fed with a standard diet with or without resveratrol (500 mg/kg). Fructose was given as 10% in drinking waterfor 24 weeks. Long-term dietary fructose caused parenchymal degeneration and hyperemia in association with impaired eNOS mRNA/protein expressions in liver of male and female rats. This dietary intervention also led to increases in hepatic triglyceride content, TNFα and IL-1β levels in both genders. Gender-related differences to consequence of fructose consumption were not obvious. Resveratrol supplementation markedly attenuated hepatic degeneration, hyperemia and triglyceride content in association with reduced TNFα and IL-1β levels, but enhanced IRβ mRNA and IRS-1 protein, in male and female rats upon fructose feeding. Long-term dietary fructose causes hepatic degeneration possibly via a decrease in eNOS, but increase in TNFα and IL-1β, in both genders. Resveratrol supplementation improved fructose-induced hepatic injury.

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Section: Discussionmentioning

confidence: 96%

Dietary Fructose-Induced Hepatic Injury in Male and Female Rats: Influence of Resveratrol

et al. 2016

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“…Various findings [7,47,48] suggest that the optimal concentration of resveratrol for anti-inflammatory action in rat models ranges from 10 to 30 mg/kg. In the past, we also performed several studies [11,40,49,50] to demonstrate that resveratrol at 10 ~ 20 mg/kg can suppress severe acute pancreatitis and reduce inflammatory injury in rats.…”

Section: Discussionmentioning

confidence: 99%

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

Wei

Chen

Wang

et al. 2016

Cell Physiol Biochem

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Background: Intra-abdominal adhesions are a very common complication following abdominal surgery. Our previous studies have demonstrated that the inhibition of inflammation at the sites of peritoneal injury can prevent the formation of intra-abdominal adhesions. Resveratrol is a natural extract with a broad range of anti-inflammatory effects. Therefore, we propose that resveratrol can reduce the formation of intra-abdominal adhesions after surgery. The aim of this study was to investigate the effect of resveratrol on intra-abdominal adhesion prevention in a rat model with surgery-induced peritoneal adhesions. Materials and Methods: The cecum wall and its opposite parietal peritoneum were abraded following laparotomy to induce intra-abdominal adhesion formation. Varying doses of resveratrol were administered to the animals. On the eighth day after surgery, the adhesion score was assessed using a visual scoring system. Picrosirius red staining and a hydroxyproline assay were used to assess the amount of collagen deposition in the adhesion tissues. The levels of serum interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and transforming growth factor beta-1 (TGF-β1) were determined by an enzyme-linked immunosorbent assay (ELISA). Western blotting was performed to determine the protein expression of TGF-β1, fibrinogen, and α-smooth muscle actin (α-SMA) in rat peritoneal adhesion tissue. Real-time RT-PCR was performed to quantify the mRNA expression of TGF-β1, fibrinogen, and α-SMA. Results: Resveratrol significantly reduced intra-abdominal adhesion formation and fibrin deposition in the rat model. Furthermore, resveratrol significantly reduced the serum levels of IL-6, TNF-α, and TGF-β1. The protein and mRNA expression of TGF-β1, fibrinogen, and α-SMA in the rat peritoneum and adhesion tissues were also down-regulated due to resveratrol intervention. Conclusion: Resveratrol can effectively prevent the formation of postoperative intra-abdominal adhesions in a rat model. This effect may be related to the suppression of inflammatory cytokine expression in the injured peritoneum by resveratrol. This study suggests that resveratrol may be a new and effective anti-adhesive agent that is worthy of further study and has potential application value.

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“…Apoptosis is a process that causes the activation of genes and enzymes in association with various physiological and pathological agents. Resveratrol has been shown to prevent apoptosis in the liver of rats with streptozotocin‐induced diabetes and in a CCl 4 hepatotoxicity model . Exposure to α‐AMA for 12 hr in primary dog hepatocyte cell cultures has been reported to cause a decrease in cell vitality proportional to the concentration of α‐AMA and length of exposure.…”

Section: Discussionmentioning

confidence: 99%

A Comparison of the Effectiveness of Silibinin and Resveratrol in Preventing Alpha‐Amanitin‐Induced Hepatotoxicity

Şahin

Arıcı

Yılmaz

et al. 2018

Basic Clin Pharma Tox

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Amanita phalloides species mushrooms containing alpha-amanitin (α-AMA) are responsible for the majority of fatal mushroom intoxications and can lead to severe poisonings resulting in hepatotoxicity and acute hepatic failure. Existing antidotes, such as silibinin, are not sufficiently effective in the prevention and/or resolution of α-AMA-induced hepatotoxicity. We investigated the effects of resveratrol on α-AMA-induced hepatotoxicity and compared with silibinin, a known antidote using in vivo and in vitro toxicity models. In the in vivo protocol, resveratrol (30 mg/kg) was given simultaneously with α-AMA (α-AMA + SR) or 12 (α-AMA + 12R) or 24 (α-AMA + 24R) hr after α-AMA administration. Silibinin (5 mg/kg) (α-AMA + Sil) and normal saline (α-AMA + NS) were given simultaneously with α-AMA. We found that liver transaminase levels in α-AMA + SR and α-AMA + 12R groups and histomorphologic injury score in the α-AMA + SR, α-AMA + 12R, α-AMA + 24R and α-AMA + Sil groups were significantly lower than that of the α-AMA + NS group. Resveratrol decreased mononuclear cell infiltration, necrosis and active caspase-3 immunopositivity in the liver. In the in vitro protocol, the effects of resveratrol and silibinin were evaluated in a reduction in cell viability induced by α-AMA in THLE-2 and THLE-3 hepatocytes. Neither resveratrol nor silibinin was found to be effective in increasing cell viability decreased by α-AMA + NS. As a conclusion, resveratrol was found to be effective in α-AMA-induced hepatotoxicity with its anti-inflammatory properties in in vivo conditions. It is a promising compound with the potential for use in the treatment of hepatotoxicity associated with Amanita phalloides type mushroom poisonings.

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Resveratrol Ameliorates the Components of Hepatic Inflammation and Apoptosis in a Rat Model of Streptozotocin‐Induced Diabetes

Cited by 18 publications

References 30 publications

Dietary Fructose-Induced Hepatic Injury in Male and Female Rats: Influence of Resveratrol

Dietary Fructose-Induced Hepatic Injury in Male and Female Rats: Influence of Resveratrol

Effect of Resveratrol on the Prevention of Intra-Abdominal Adhesion Formation in a Rat Model

A Comparison of the Effectiveness of Silibinin and Resveratrol in Preventing Alpha‐Amanitin‐Induced Hepatotoxicity

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