Resveratrol Ameliorates Testicular Histopathology of Mice Exposed to Restraint Stress

Abstract

Simple SummaryThe search for effective medicines is challenging. Resveratrol is a phytoalexin, and its function remains unelucidated. Therefore, we undertook the present study to investigate reproductive disturbances due to restraint stress in mice and whether resveratrol plays an anti-stress role. Our results confirmed that resveratrol plays a potential role in the reduction of stress in mice. AbstractWe evaluated immobilization stress and resveratrol supplementation in immature male mice at 30 days of age for 15 consecutive days. Fifty Swiss mice were divided into five groups (10 mice each): Controls, restraint stress (RS), restraint stress + vehicle (RS + V), RS + 2 mg/kg, and RS + 20 mg/kg. We determined results on the basis of hematoxylin and eosin (H&E), “Periodic acid-Schiff” staining, and TUNEL assay. The results indicated that immobilization stress significantly decreased body weight, testis weight, and water/food intake compared to the control; while resveratrol ameliorated these effects. The quantitative histologic evaluation of the seminiferous tubule diameter, luminal diameter, area of seminiferous tubules, area of tubule lumen, epithelial height, Leydig cell number, and the width of the tunica albuginea were similarly decreased after exposure to RS. These parameters recovered back to normal in the RS + 2 mg/kg group. The development of spermatogenesis was significantly delayed in the RS, RS + V, and RS + 20 mg groups based upon our evaluation score system. However, we observed no significant differences in the RS + 2 mg group compared with the control group. The number of TUNEL-positive cells also significantly decreased in the RS + 2 mg/kg group. In conclusion, we found that the administration of 2 mg/kg was an effective dose against immobilization stress in mice.

MOTILIPERM Ameliorates Immobilization Stress-Induced Testicular Dysfunction via Inhibition of Oxidative Stress and Modulation of the Nrf2/HO-1 Pathway in SD Rats

“…The decrease in germ cell number was due to downregulation of testosterone levels. These results are consistent with our previous findings [29]. This study demonstrates that MOTILIPERM restores seminiferous tubular structure, protects germ cell proliferation, and improves sperm count and sperm motility.…”

“…The present investigation also showed that testicular apoptosis is significantly increased in stressed rats, which is consistent with previous findings [1,3,11,15,29,45]. Bax is an important pro-apoptotic gene; Bcl-2 is an anti-apoptotic gene; and cleaved caspase-3 is an executioner caspase that is essential for triggering apoptosis [24].…”

Positive Roles of Resveratrol in Early Development of Testicular Germ Cells against Maternal Restraint Stress in Mice

“…2) (A = 3.14 √ 2) [33]. In order to compare histopathological parameters and MRS values, one-way analysis of variance [37] results for multiple comparison tests by Dennett's multiple comparisons (which compares the mean of each column with the mean of control column) were carried out.…”

“…Resveratrol (Sigma Chemical Co., St Louis, MO, USA) was diluted to 5% in the vehicle (CMC, sodium carboxymethyl cellulose) [33,34]. The Beijing Institute for Pharmacology and Toxicology (China) supplied sodium carboxymethyl cellulose with trans-resveratrol for oral gavage at 0.5% in CMC.…”

Morphometric Evaluation of Spermatogenic Cells and Seminiferous Tubules and Exploration of Luteinizing Hormone Beta Polypeptide in Testis of Datong Yak

“…Our findings illustrated that luminal diameter, luminal area, Leydig cell area, and width of tunica albuginea increased as yaks aged toward sexual maturity. Sheeraz et al [46] evaluated luminal diameter (69.10 ± 16.99 µm 2 ), luminal area (49.26 ± 8.47 µm 2 ), Leydig cell area (108.19 ± 30.60 µm 2 ), and width of tunica albuginea (12.99 ± 1.35 µm) in mice. In our findings, the mean cross-sectional area of the seminiferous tubules varied from 46,675.0 ± 2689 to 50,812 ± 3711 µm 2 for different ages of yak, and the differences were not significantly different.…”