Resveratrol alleviates imidacloprid‐induced mitochondrial apoptosis, necroptosis, and immune dysfunction in chicken lymphocyte lines by inhibiting the ROS/MAPK signaling pathway

Abstract

Imidacloprid (IMI) is a neonicotinoid insecticide with the highest global market share, and IMI exposure in the environment can negatively affect many nontarget organisms (a general term for organisms affected by drugs other than target organisms). Resveratrol (RSV), a non‐flavonoid polyphenolic organic compound derived from peanuts, grapes, and other plants, has anti‐inflammatory and antioxidant effects. It is currently unclear how RSV protects against cell damage caused by IMI. Therefore, we established an experimental model of chicken lymphocyte lines exposed to 110 μg/mL IMI and/or 0.5 μM RSV for 24 h. According to the experimental results, IMI markedly raised intracellular reactive oxygen species levels and diminished the activity of the cellular antioxidant enzymes (CAT, SOD, and GPx), leading to MDA accumulation and decreased T‐AOC. JNK, ERK, and P38, the essential components of the mitogen‐activated protein kinase (MAPK) signaling pathway, were also expressed more when IMI was present. Additionally, IMI resulted in upregulation of mitochondrial apoptosis (Caspase 3, Caspase 9, Bax, and Cyt‐c) and necroptosis (Caspase 8, RIPK1, RIPK3, and MLKL) related factors expression, downregulation of Bcl‐2 expression, induction of upregulation of cytokine IL‐6 and TNF‐α expression, and downregulation of IFN‐γ expression. The combined treatment of RSV and IMI significantly reduced cellular oxidative stress levels, inhibited the MAPK signaling pathway, and alleviated IMI‐induced mitochondrial apoptosis, necroptosis, and immune dysfunction. To summarize, RSV antagonized IMI‐induced mitochondrial apoptosis, necroptosis, and immune dysfunction in chicken lymphocyte lines by inhibiting the ROS/MAPK signaling pathway.

Inhalation exposure-induced toxicity and disease mediated via mTOR dysregulation

“…This highly reactive oxygen containing species induces mitochondrial dysfunction and stimulates various signaling proteins kinases. These oxidative damage conditions can initiate autophagosome formation inducing autophagy [ 44 , 45 ]. In biological system, NPs tends to form protein-NP corona depending on protein affinity and NPs characteristics.…”

Dietary composition influences sperm quality and testis damage via endoplasmic reticulum stress in lambs

“…Wang et al. ( 2024 ) reported that the imidacloprid insecticide triggers mitochondrial apoptosis (Caspase‐3, Caspase‐9, Bax and Cyt‐c), necroptosis (Caspase‐8, RIPK1, RIPK3 and MLKL) and immune dysfunction in chickens by activating the key components of the mitogen‐activated protein kinase signalling pathway, resulting in oxidative stress. They also observed that these dysfunctions are significantly reduced by the polyphenolic compound Resveratrol.…”