Resveratrol, a Polyphenol Found in Wine, Reduces Ischemia Reperfusion Injury in Rat Kidneys

Abstract

Reactive oxygen species have been implicated in the pathophysiology of renal ischemia reperfusion injury. Antioxidants including polyphenolics have been found to protect renal cells from the cellular injury induced by ischemia and reperfusion. Resveratrol, a stilbene polyphenol found in grapes and red wine, has recently been found to protect isolated rat heart from ischemia reperfusion injury. This study was sought to determine if resveratrol could also protect renal cells from ischemic injury. Male Wistar rats were treated with control, resveratrol (0.23 microg/kg), vehicle used to solubilize resveratrol, and resveratrol plus L-NAME (15 mg/kg body wt), a nitric oxide blocker. Our results demonstrated that resveratrol administration reduced the mortality of ischemic rats from 50% to 10% and renal damage was reduced as indicated by histologic examination and serum creatinine level. The short-term administration of resveratrol also inhibited renal lipid peroxidation induced by ischemia and reperfusion both in cortex and in medulla. Electron paramagnetic resonance detected an increased formation of nitric oxide in the resveratrol-treated kidney that was reduced to the baseline value after treating the rats with L-NAME in addition to resveratrol. The results suggest that resveratrol reduced the renal ischemia reperfusion injury through a nitric oxide-dependent mechanism.

Bone Marrow-Derived Cells Mobilized by Granulocyte-Colony Stimulating Factor Facilitate Vascular Regeneration in Mouse Kidney after Ischemia/Reperfusion Injury

“…Resveratrol has been shown to restore kidney function in I/R injury in animal experiments (Giovannini et al 2001;Saito et al 2005), although our study on mice failed to demonstrate a protective effect. Giovannini et al (2001) blocked rat renal arteries for 40 minutes and administered resveratrol at 0.23 μ g/kg, while Saito et al (2005) blocked rat renal arteries for 60 min and administered resveratrol at 4 mg/kg.…”

“…Giovannini et al (2001) blocked rat renal arteries for 40 minutes and administered resveratrol at 0.23 μ g/kg, while Saito et al (2005) blocked rat renal arteries for 60 min and administered resveratrol at 4 mg/kg. They both found decreased serum creatinine levels by resveratrol compared with the vehicle.…”

Kidney-specific Overexpression of Sirt1 Protects against Acute Kidney Injury by Retaining Peroxisome Function

“…Sirt1, a mammalian homologue of Sir2, acts on several nuclear proteins, including p53 (2) and forkhead family proteins (FoxOs) (3), and prevents apoptosis. Calorie restriction or resveratrol, which activates Sirt1, is reported to protect from reactive oxygen species (ROS)-induced 2 organ insult (4,5). Additionally, the overexpression of Sirt1 itself also strengthens the stress resistance.…”

Inhaled Hydrogen Gas Therapy for Prevention of Renal Ischemia/Reperfusion Injury in Rat Model

“…Antioxidants have been found to protect renal cells from cellular injury induced by I/R. [22][23][24] The finding of the anti-oxidant effect of hydrogen and its therapeutic value led us to suggest that endogenous hydrogen may play an important physiological role in maintaining homeostasis. Several studies have shown that hydrogen exerts cytoprotective effects after various types of injuries.…”