Resuscitation-promoting factors are important determinants of the pathophysiology in<i>Mycobacterium tuberculosis</i>infection

Rosser, Andrew; Stover, Cordula M.; Pareek, Manish; Mukamolova, Galina V.

doi:10.1080/1040841x.2017.1283485

Cited by 59 publications

(43 citation statements)

References 93 publications

(158 reference statements)

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“…Thus, VBNC or uncultured bacteria in cellulosic biomass must be able to respond to Rpf, otherwise they will miss chances for growth and division. However, until recently, little is known about the potential cellulase activities of uncultured bacteria, as previous studies have focused on resuscitation of VBNC bacteria from the view of medicine and epidemiology (Mukamolova et al, 2002;Oliver, 2010;Rosser et al, 2017). Research is needed to explore the uncultivated bacteria which are potential cellulose degraders using the resuscitation and stimulation function of Rpf.…”

Section: Introductionmentioning

confidence: 99%

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Zhang

Mei

et al. 2018

Microbial Biotechnology

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SummaryNowadays, much of what we know regarding the isolated cellulolytic bacteria comes from the conventional plate separation techniques. However, the culturability of many bacterial species is controlled by resuscitation‐promoting factors (Rpfs) due to entering a viable but non‐culturable (VBNC) state. Therefore, in this study, Rpf from Micrococcus luteus was added in the culture medium to evaluate its role in bacterial isolation and enhanced effects on cellulose‐degrading capability of bacterial community in the compost. It was found that Proteobacteria and Actinobacteria were two main phyla in the compost sample. The introduction of Rpf could isolate some unique bacterial species. The cellulase activity of enrichment cultures with and without Rpf treatment revealed that Rpf treatment significantly enhanced cellulase activity. Ten isolates unique in Rpf addition displayed carboxymethyl‐cellulase (CMCase) activity, while six isolates possessed filter paper cellulase (FPCase) activity. This study provides new insights into broader cellulose degraders, which could be utilized for enhancing cellulosic waste treatment.

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Section: Introductionmentioning

confidence: 99%

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Zhang

Mei

et al. 2018

Microbial Biotechnology

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“…Up to now, the resuscitation function of Rpf proteins has been extensively documented in the fields of medical and food microbiology (Oliver ; Rosser et al . ). However, very little research work has been done on resuscitation of VBNC bacteria for isolating high‐efficient pollutant‐degrading bacteria.…”

Section: Resuscitation Of Vbnc Bacteria In Pah‐contaminated Sitesmentioning

confidence: 97%

Revealing potential functions of VBNC bacteria in polycyclic aromatic hydrocarbons biodegradation

Bamba

Zhang

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: As the vast majority microbial resource, viable but nonculturable (VBNC) bacteria, which showed their potential functions in polycyclic aromatic hydrocarbons (PAHs) biodegradation, can be of great significance in environmental bioremediation. It is therefore important to resuscitate VBNC bacteria for their better capabilities. Meanwhile, preventing the indigenous functional community from entering into the VBNC state will also maintain the high activity of PAH-degrading bacteria in actual bioremediation applications. Undoubtedly, much more work needs to be done to reveal indigenous micro-organisms in the VBNC state from the perspective of environmental functions. AbstractThe bioremediation of polycyclic aromatic hydrocarbon (PAH)-contaminated sites is not running smoothly, because of the lower activity of PAH-degrading bacteria in actual bioremediation applications. The phenomenon of "viable but nonculturable" (VBNC) state may be a main limiting factor for their poor biodegradation capabilities of PAHs. Due to their abilities of entering into the VBNC state, most of bacterial populations with PAH-degradation potential remain unculturable. Resuscitation of VBNC bacteria will enhance the degradation capability of indigenous bacteria which will eventually obtain their better capabilities in environmental bioremediation. Although evidences have been presented indicating that resuscitation of VBNC bacteria in polychlorinated biphenyl (PCB)-contaminated environments not only significantly enhanced PCB degradation, but also obtained novel highly efficient PCB-degrading bacteria, scanty information is available on the VBNC bacteria in PAH-contaminated sites. VBNC bacteria, as a vast majority of potential microbial resource could be the repository of novel highly efficient PAH-biodegraders. Therefore, studies need to be done on resuscitation of VBNC bacteria to overcome key bottlenecks in bioremediation of PAHcontaminated sites. This mini-review provides a new insight into the potential functions of VBNC bacteria in PAHs biodegradation.

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“…The ability to switch between actively replicating and non-replicating persistence (NRP) is at the heart of Mycobacterium tuberculosis’ success as a pathogen. M. tuberculosis expresses five resuscitation-promoting factors (RpfA-E) (1). These are cell wall remodelling enzymes critical for the transition of M. tuberculosis between dormancy and resuscitation, and for reactivation of tuberculosis (TB) in animal models (2-4).…”

Section: Introductionmentioning

confidence: 99%

Cell-wall synthesis and ribosome maturation are co-regulated by an RNA switch in Mycobacterium tuberculosis

Schwenk

Moores

Nobeli³

et al. 2017

Preprint

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The success of Mycobacterium tuberculosis as a pathogen relies on the ability to switch between active growth and non-replicating persistence, associated with latent TB infection. Resuscitation promoting factors (Rpfs) are essential for the transition of M. tuberculosis to dormancy and for emergence from the non-replicating persistent state. But these enzymes are double-edged swords, as their ability to degrade the cell wall, is potentially lethal to the bacterium itself. Hence, Rpf expression is tightly regulated. We have identified a novel regulatory element in the 5’ untranslated region (UTR) of rpfB. We demonstrate that this element is a transcriptionally regulated RNA switch/riboswitch candidate, which is restricted to pathogenic mycobacteria, suggesting a role in virulence. Moreover, we have used translation start site mapping to re-annotate the RpfB start codon and identified and validated a ribosome binding site that is likely to be targeted by an RpfB antisense RNA. Finally, we show that rpfB is co-transcribed with downstream genes, ksgA and ispE. ksgA encodes a universally conserved methyl transferase involved in ribosome maturation and ispE encodes an essential ATP-dependent kinase involved in cell wall synthesis. This arrangement implies co-regulation of resuscitation, cell wall synthesis and ribosome maturation via the RNA switch. We propose that deregulation of this switch, associated with cell wall synthesis and ribosome function, presents a new target for anti-tuberculosis drug development.ImportanceThis work describes the identification and characterisation of a novel regulatory RNA element/attenuator that controls cell wall synthesis and ribosome function in Mycobacterium tuberculosis, the causative agent of human tuberculosis (TB). By switching between two different conformations, this RNA switch can either enable or inhibit transcription of a tri-cistronic mRNA that encodes a cell-wall remodelling enzyme crucial for activation of latent TB, an RNA methytransferase that is important for ribosome function and a protein kinase essential for early steps in cell wall synthesis. This RNA switch is only present in a subset of pathogenic mycobacteria, and by regulating the expression of three genes associated with classical antimicrobial targets we believe that it offers a novel important target for future anti-tuberculosis drugs.

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Resuscitation-promoting factors are important determinants of the pathophysiology inMycobacterium tuberculosisinfection

Cited by 59 publications

References 93 publications

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Resuscitation of viable but non‐culturable bacteria to enhance the cellulose‐degrading capability of bacterial community in composting

Revealing potential functions of VBNC bacteria in polycyclic aromatic hydrocarbons biodegradation

Cell-wall synthesis and ribosome maturation are co-regulated by an RNA switch in Mycobacterium tuberculosis

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