Results of mosquito collection at four areas near Narita International Airport, Japan, in 2003 and 2004

良夫, 津田; 由紀子, 比嘉; 真治, 葛西; 晴彦, 伊澤; 啓太, 星野; 利彦, 林; 修, 駒形; 京子, 澤邉; 年則, 佐々木; 隆史, 冨田; 直子, 二瓶; 弘, 倉橋; 陸生, 小林

doi:10.7601/mez.57.211

Cited by 9 publications

(1 citation statement)

References 18 publications

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“…p. pallens and Cx. p. form molestus are the major species of Culex mosquitoes collected in the field (29,30) and the anautogenous type of Cx. p. pipiens has yet to make an appearance in this country.…”

Section: Resultsmentioning

confidence: 99%

PCR-Based Identification of <i>Culex pipiens</i> Complex Collected in Japan

et al. 2008

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The Culex pipiens complex consists of vector mosquitoes that transmit important human pathogens. In this study we established a simplified method to distinguish three members of the Cx. pipiens complex, Cx. p. pallens Coquillet, Cx. p. form molestus Forskal, and Cx. quinquefasciatus Say, collected in Japan. Sequence analysis of the Drosophila Ace-orthologous acetylcholinesterase (Ace) gene (668 to 680 bp) revealed that a single polymorphic region characterizes each species. Based on this region, specific primers that distinguish Cx. p. form molestus (ACEpip2) and Cx. p. pallens (ACEpall2) were newly designed. Polymerase chain reactions were performed with the genomic DNA of Culex mosquitoes as the template, and these primers clearly distinguished two Culex spp. The accuracy of the designed primers was evaluated with 38 colonies of mosquito samples collected from 9 prefectures of Japan. The testing revealed that the distribution of anautogenous Cx. p. pipiens has not been confirmed in Japan. It also revealed that the male of Cx. p. pallens possesses an Ace gene haplotype that is highly similar to the sequence of Cx. quinquefasciatus. This improved method allows the evaluation of vector competence of Cx. p. form molestus, which is the suspected vector of West Nile virus.

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Section: Resultsmentioning

confidence: 99%

PCR-Based Identification of <i>Culex pipiens</i> Complex Collected in Japan

et al. 2008

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The inhibitory effect of a combination of imidacloprid and permethrin on blood feeding by mosquitoes in dogs raised under outdoor conditions

Machida¹,

Kondo²,

Kanehira³

et al. 2008

Veterinary Parasitology

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Host-Feeding Habits ofCulex pipiensandAedes albopictus(Diptera: Culicidae) Collected at the Urban and Suburban Residential Areas of Japan

et al. 2010

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To evaluate the vectorial capacity of mosquitoes for viruses in Japan, the host-feeding habits of the mosquitoes were analyzed by sequencing polymerase chain reaction-amplified fragments of the cytochrome b and 16S ribosomal RNA regions of the mitochondrial DNA of 516 mosquitoes of 15 species from seven genera that were collected from residential areas during 2003-2006. Culex pipiens L. and Aedes albopictus Skuse were the most commonly collected species in urban and suburban residential areas. Anautogenous Culex pipiens pallens Coquillett was distinguished from the autogenous Cx. pipiens form molestus Forskal using a polymerase chain reaction-based identification method. Both Cx. p. pallens and Cx. p. form molestus exhibited similar host-feeding habits, broadly preferring avian (50.0 and 42.5% of avian, respectively) and mammalian (38.6 and 45.0% of avian, respectively) hosts, such as tree sparrows, ducks, and humans. Conversely, Ae. albopictus exhibited a highly mammalophilic and anthropophilic feeding pattern, with 84.2% feeding on mammalian hosts and 68.5% of these on humans. We concluded that in Japan, Cx. pipiens might play a significant role in the avian-to-mammal transmission of viruses, such as West Nile virus, whereas Ae. albopictus might play a role in the human-human transmission of dengue and Chikungunya viruses.

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Results of mosquito collection at four areas near Narita International Airport, Japan, in 2003 and 2004

Cited by 9 publications

References 18 publications

PCR-Based Identification of <i>Culex pipiens</i> Complex Collected in Japan

PCR-Based Identification of <i>Culex pipiens</i> Complex Collected in Japan

The inhibitory effect of a combination of imidacloprid and permethrin on blood feeding by mosquitoes in dogs raised under outdoor conditions

Host-Feeding Habits ofCulex pipiensandAedes albopictus(Diptera: Culicidae) Collected at the Urban and Suburban Residential Areas of Japan

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