Results of intradermal tests in horses without atopy and horses with chronic obstructive pulmonary disease

Lorch, Gwendolen; Hillier, Andrew; Kwochka, Kenneth W.; Saville, William J.; Kohn, Catherine W.; Jose‐Cunilleras, Eduard

doi:10.2460/ajvr.2001.62.389

Cited by 36 publications

(35 citation statements)

References 22 publications

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“…T h e y are comparable to previous results, but the security coefficient is much higher than previously. Note the significant positive d i fference between healthy horses and horses with allerg i c respiratory disease to C u l e x and Dermatophagoides farinae, w h i c h is different from that observed by Lorch et al (2001c). A great part of the differences found previously according to age, season or breed have disappeared.…”

Section: Reading Criteria After 20 Minutesmentioning

confidence: 62%

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Intradermal skin tests in equine dermatology: a study of 83 horses

Lebis¹,

Bourdeau

Marzin-Keller³

2002

Equine Veterinary Journal

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Summary Allergic diseases are often diagnosed clinically in the horse without performing diagnostic tests. The purpose of this work was to contribute to the validation of intradermal skin tests in the horse. Eighty‐three horses, 14 showing skin or respiratory signs of supposed allergic origin, were subjected to an intradermal skin test using 6 different allergens, positive and negative controls. The tests were read for all animals after 20 min, and for 29 horses after 1 and 4 h. Additionally, 19 horses were tested a few months apart. The comparison after 20 min of the cutaneous reactions to allergens and to positive and negative controls allowed us to propose a threshold of positivity; skin reactions with a diameter above or equal to 15 mm, or with at least a 13 mm diameter and a skin thickness similar to the positive control. There was a marked difference between the healthy group and the allergic group for Culex pipiens and Dermatophagoides farinae, although positive reactions were not rare in the healthy group. Tabanus sp. gave positive reactions in both healthy and allergic animals. There was no significant variation in the reactions observed after 20 min and after 60 min. After 4 h, the progression of the reactions was highly variable and negative controls showed a certain number of positive reactions, which negated their reliability at the point in time, and made it difficult to interpret the other allergens. Finally, the repeatability of intradermal skin tests reactions after 20 min was poor, and probably related to the influence of seasonality for some of the allergens. Further studies are required, notably with others allergens, to interpret intradermal skin tests' responses in clinical practice.

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Section: Reading Criteria After 20 Minutesmentioning

confidence: 62%

“…Sloet Van Oldruitenborg h - Oosterban et al (1996) proposed the use of these allergens at lower dilution. Lorch et al (2001c) considered that allergens with a positive test result in >25% of normal horses were irritants.…”

Section: Results At 20 Minmentioning

confidence: 99%

Intradermal skin tests in equine dermatology: a study of 83 horses

Lebis¹,

Bourdeau

Marzin-Keller³

2002

Equine Veterinary Journal

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“…Horses with atopic dermatitis and recurrent hives generally have a higher incidence of positive reactions than healthy horses, but the diagnosis (as in other species) cannot solely be made on the basis of the intradermal test or serological testing alone (Lorch et al . ,b,c). The testing should be interpreted in light of the history of the disease and used to determine which allergens might be useful in ASIT, should the owners elect that route of treatment.…”

Section: Allergiesmentioning

confidence: 99%

A diagnostic approach to the pruritic horse

White

2015

Equine Veterinary Education

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Summary Pruritus is a common complaint in equine medicine. While pruritus in most horses is due to ectoparasites (midges, flies, mites) or environmental allergens (pollens, barn dust, moulds, etc.), other causes such as staphylococcal or fungal infections, vasculitis, and internal organ dysfunction should not be overlooked. In a rational approach to the pruritic horse, history and physical examination become very important as a guide to choosing diagnostic tests.

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“…Actually, few studies have been performed with monoclonal antibodies (Wagner et al 2003) or with the α chain of the IgE receptor (Lorch et al 2001a) to allow specific detection of equine IgE. Additionally, direct testing of the horse's skin has not proven useful for diagnostic purposes since most horses give a positive skin reaction to common barn allergens, even though horses with RAO tend to have a stronger positive skin reaction (Eyre 1972;Schatzmann and Gerber 1972;Evans et al 1992;Mcgorum et al 1993;Lorch et al 2001b). Furthermore, another study suggests that a higher concentration of A. fumigatus mixture in the skin test may be needed to detect horses sensitive to this antigen group (Wong et al 2005).…”

Section: Introductionmentioning

confidence: 99%

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with Recurrent Airway Obstruction

et al. 2009

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In horses, Recurrent Airway Obstruction (RAO) is an allergic disease that involves IgE mediated Type I Hypersensitivity responses. The development of this type of allergy involves a series of events that begins with reaginic antibodies, mainly IgE and some IgG subclasses. These reaginic antibodies bind with high affinity, via the Fc portion, to FcepsilonRI receptors on the membrane of mast cells and basophils. Once bound, environmental allergens cross-link the antibodies, which results in mast cell degranulation leading to the production of histamine and other chemical mediators that act together to induce airway inflammation. RAO-affected horses present with coughing, respiratory distress, airway obstruction and poor performance. The aspect of the RAO has been extensively studied, yet the precise sequence of events is still not well understood. Therefore, this study proposes a bioassay for reaginic antibody detection from horse serum of RAO-affected individuals, in order to determine the etiology of disease, which mediate immediate type reactions. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from affected or unaffected horses and one of the RAO antigens (Aspergillus fumigatus). The results presented here demonstrate that 30% of RAO-affected horses react positively in this in vitro bioassay, whereas unaffected horses do not. This bioassay may facilitate further research on RAO and other allergic diseases in horses.

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Results of intradermal tests in horses without atopy and horses with chronic obstructive pulmonary disease

Cited by 36 publications

References 22 publications

Intradermal skin tests in equine dermatology: a study of 83 horses

Intradermal skin tests in equine dermatology: a study of 83 horses

A diagnostic approach to the pruritic horse

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with Recurrent Airway Obstruction

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