Results of ecological investigation on Culicoides nipponensis TOKUNAGA, 1955 in Meiwa-cho, Taki-gun, Mie Prefecture

勝己, 丸山; 正洋, 高木

doi:10.7601/mez.28.367

Cited by 2 publications

(1 citation statement)

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“…Earlier reports also revealed immature of C. arakawae, C. oxystoma, C. nipponensis, and C. peregrinus in paddy Þelds (Kitaoka and Morii 1963, Maruyama and Takagi 1977, Wirth and Hubert 1989, but this is the Þrst report of larvae of C. kibunensis, C. pictimargo, C. punctatus, and C. verbosus in paddy Þelds as far as we know. Larvae of C. arakawae and C. kibunensis were also collected from the surface mud of a paddy Þeld at Kagoshima A in early March, when adult midges were not active, suggesting that their larvae overwintered.…”

Section: Discussionmentioning

confidence: 63%

Molecular Identification of Field-CollectedCulicoidesLarvae in the Southern Part of Japan

et al. 2013

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Although Culicoides biting midges act as a vector of important human and domestic animal diseases, their ecology is poorly understood. The lack of proper identification systems of Culicoides larvae is one of the main obstacles to progress in research. Based on mitochondrial sequences of 19 Japanese Culicoides species, we designed a universal primer set to amplify the partial sequence of the mitochondrial cytochrome c oxidase I (cox 1). The polymerase chain reaction product amplified from extracted DNA of Culicoides larvae using the primer set was directly sequenced, and species identification based on the variation at cox1 was conducted. Using the molecular identification system, we sorted 243 specimens of field-collected larvae from the southern part of Japan into 10 species including Culicoides arakawae (Arakawa), Culicoides oxystoma Kieffer, and Culicoides brevitarsis Kieffer, which are regarded as vectors of important livestock animal diseases. Eight species of Culicoides larvae, including C. arakawae and C. oxystoma, were recovered from active paddy fields and an abandoned paddy field. The result suggests that paddy fields contribute to breeding a variety of Culicoides species and maintenance and spread of Culicoides-borne pathogens. In contrast, larvae of C. brevitarsis were collected from cattle dung in pastures. The molecular identification system described herein using nucleotide sequences successfully achieved larval identification and will be useful for a better understanding of larval habitats of Culicoides biting midges.

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Section: Discussionmentioning

confidence: 63%