Results of a Decellularized Porcine Heart Valve Implanted into the Juvenile Sheep Model

Dohmen, Pascal M.; Costa, Francisco da; Lopes, Sergio Veiga; Yoshi, S.; Souza, F.P. da; Vilani, Ricardo; Costa, Maurício Barcelos; Konertz, Wolfgang

doi:10.1532/hsf98.20041140

Cited by 59 publications

(46 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Grauss et al [7] showed that changes in formation of extracellular matrix after chemical decellularization of the graft could lead to problems in the cell repopulation by cells of host origin, mainly depending on the method used for decellularization. The use of sodium deoxycholate was previously reported as a suitable method for decellularization of porcine pulmonary and aortic valves [11]. In this study, the cell extraction by using sodium deoxycholate resulted in no significant structural changes in configuration of the collagen fibers of the extracellular matrix, which is important to offer a suitable environment for the in vivo process of remodeling of the valve.…”

Section: Discussionmentioning

confidence: 79%

Análise do comportamento biológico de heteroenxertos descelularizados e homoenxertos criopreservados: estudo em ovinos

Lopes¹,

Costa²,

Paula³

et al. 2009

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

Análise do comportamento biológico de heteroenxertos descelularizados e homoenxertos criopreservados: estudo em ovinosDecellularized heterografts versus cryopreserved homografts: experimental study in sheep model Abstract Objectives: The aim of this study is to assess the biological behaviour of porcine decellularized heterografts (Desc group) compared with cryopreserved homografts (Crio group) implanted in juvenile sheep.Methods: Decellularized porcine pulmonary heterografts were implanted in five animals and cryopreserved pulmonary homografts in another five. The animals were followed-up for a mean of 280 ± 14 days. The valve diameter was measured by echocardiography, which was performed at the 30th postoperative day, and before the explantation. The valves were also assessed macroscopically. Histological evaluation was performed using H.E., Gomori and Weigert staining. Immunohistochemistry specified different cell types (Factor VIII, CD3, Vimentin and CD68). Calcium quantity was analyzed using atomic absortion spectometry.Results: There was one death in the Desc group due to endocarditis. The valves of Crio group showed decrease in the cellularity whereas the valves of Desc group showed matrix repopulation with endothelial and interstitial cells. Loss of collagen density and disarrangement of the normal fiber architecture was observed in Crio group. Calcium content demonstrated higher levels on the cusps and conduits in Crio group comparatively with Desc group. (P=0.016). The mean valvular diameter at the explantation was significantly increased (P=0.025) in the Desc group. Rev Bras Cir Cardiovasc 2009; 24(1): 15-22 Conclusions: Decellularized heterografts had a different biological behaviour when compared to cryopreserved homografts and become repopulated by cells with fibroblasts and endothelial cells characteristics. The matrix was preserved and some regenerative potential was present

show abstract

Section: Discussionmentioning

confidence: 79%

Análise do comportamento biológico de heteroenxertos descelularizados e homoenxertos criopreservados: estudo em ovinos

Lopes¹,

Costa²,

Paula³

et al. 2009

Rev Bras Cir Cardiovasc

View full text Add to dashboard Cite

show abstract

“…26 Many previous studies have suggested that the ECM may provide a suitable environment for repopulation by host cells, which would provide the ECM with a regenerative capacity typical of native valves, thus improving xenograft durability and preventing thrombosis. 14,15,29,30 However, further studies are required to investigate the capacity of the matrix to be repopulated with host endothelial and interstitial cells.…”

Section: Discussionmentioning

confidence: 99%

“…12 Enzymatic removal of cells and cellular remnants significantly reduces immunologic reaction, 13 whereas the intact ECM scaffold is expected to improve recolonization of decellularized conduits with native cells, as experimental evidence exists that the decellularized matrix becomes populated with functional native cells. [14][15][16] To our knowledge, this is the first study to analyze 3 different methods of heart valve tissue decellularization using collagenase enzyme. Immuno logic responses against decellularized tissue after implantation, as well as the tissue construction, differed with these 3 methods.…”

Section: Introductionmentioning

confidence: 99%

Untitled

Abdolghafoorian

Farnia²,

Rs³

et al. 2017

Exp Clin Transplant

View full text Add to dashboard Cite

Objectives: Endothelial cells harbor many antigenic determinants that may be targets for the immune system. The aim of this study was to determine the immunologic effects of decellularization, using 3 different methods, on xenograft rejection. Materials and Methods: In a sterile plate containing phosphate-buffered saline, fresh sheep aortic heart valves were decellularized using 3 different enzymatic methods: with 900 µg/mL of collagenase at 40°C (method A), with 450 µg/mL of collagenase at 4°C (method B), and with 900 µg/mL of collagenase at 4°C (method C). Intact and decellularized valves were implanted subdermally into inbred male albino rabbits and extracted after 21 days (extra valve pieces were also extracted after 60 days, as control samples, for assessing chronic rejection). Valves were histologically analyzed for inflammatory cell infiltration. Subendothelial structure integrity was determined using surface electron microscope. Results: No inflammatory cell infiltration was seen around the decellularized valve with method A, and no subendothelial structure change was observed by surface electron microscope. Infiltration of immune cells involved in rejection was not seen around valves decellularized with method B, although the subendothelial structure was relatively preserved and valve stiffness was increased. With method C, we observed a foreign body-type reaction around the intact valve and the decellularized valve. Conclusions: Method A is considered the optimal method of decellularization in our study, as this method significantly reduced the immune response to xenograft tissue, while maintaining subendothelial tissue.

show abstract

“…Under sterile conditions, the skin incision was made from the medial point of the costal arch to near the sternum (ventro-lateral approach), like described previously by DOHMEN et al (2005). The third or fourth intercostals spaces (ICS) were accessed with ligation and transection of the left mammary artery and vein.…”

Section: Methodsmentioning

confidence: 99%

Substituição valvar cardíaca em ovinos – Descrição da técnica operatória e análise dos resultados

Sillas¹,

Costa²,

Vilani³

et al. 2008

AVS

View full text Add to dashboard Cite

Visando avaliar um protocolo experimentalde pesquisa com próteses valvares cardíacas emovinos, as técnicas cirúrgicas de substituição valvarpulmonar, aórtica e mitral de 58 cirurgias cardíacas foramanalisadas. Através de procedimentos padronizadosos ovinos (idade 18 ± 7 semanas e o peso 30 ± 9.9kg) foram submetidos ao implante de próteses valvaresbiológicas. Quarenta animais receberam prótesesvalvares pulmonares, 14 animais receberam prótesesaórticas e quatro animais receberam próteses mitrais.O quarto espaço inter-costal foi considerado de eleiçãopara realização das três técnicas operatórias avaliadas.O método de circulação extracorpórea (CPB)por canulação da aorta descendente e do átrio direitofoi considerado rápido e seguro. O tempo de CPB durantea substituição valvar pulmonar foi de 34 ± 14minutos e a mortalidade 7,5%. A substituição valvaraórtica, com implante subcoronariano, teve tempo deCPB 119 ± 5 minutos e mortalidade de 57%. Durantea substituição valvar mitral o tempo de CPB foi 74 ±22 minutos e mortalidade 50%. A técnica de implantevalvar pulmonar foi muito eficiente, entretanto a altamortalidade das técnicas de implante valvar aórtico emitral, associadas à maior complexidade cirúrgica eelevado tempo de CPB, faz necessário refinamentostécnicos para melhorar o aproveitamento dos animaisnas cirurgias das valvas cardíadas esquerdas.

show abstract

Results of a Decellularized Porcine Heart Valve Implanted into the Juvenile Sheep Model

Cited by 59 publications

References 13 publications

Análise do comportamento biológico de heteroenxertos descelularizados e homoenxertos criopreservados: estudo em ovinos

Análise do comportamento biológico de heteroenxertos descelularizados e homoenxertos criopreservados: estudo em ovinos

Untitled

Substituição valvar cardíaca em ovinos – Descrição da técnica operatória e análise dos resultados

Contact Info

Product

Resources

About