Restriktion und Modifikation bei Staphylococcus aureus: Eigenschaften von Resistenzplasmiden und Prophagen

Abstract

Experiments on elimination and transfer of resistance‐plasmids in S. aureus (controlling resistance to penicillin, chloramphenicol and oxytetracycline) show that these plasmids have no restricting influence on phages used for typing of staphylococci. Prophages in lysogenic strains control a mechanism of restriction and modification which is active on phages and on chromosomal markers. The resistance‐plasmids used in these experiments are insensitive to prophage controlled restriction.

Multiply- and methicillin-resistantStaphylococcus aureusstrains isolated in the German Democratic Republic in 1985 and 1986

“…Experimental phage A994 (derived from phage 85, Witte, Nguyen van Dip & Diinnhaupt, 1986) was used; the experimental procedure is described by Witte (1976). The transductants were selected on nutrient medium L4 containing cadmium sulphate (200 ,g/ml), chloramphenicol (10 jug/ml, erythromycin (2 ,ug/ml), gentamicin (1 jug/ml) and oxytetracycline (10 ,ug/ml).…”