Restriction site patterns in the ribosomal DNA of Camelidae

Abstract

The restriction map of rDNA from South American camelids and the Bactrian camel was analyzed by digestion of high-molecular-weight DNA with endonucleases EcoRI,BamHI and the two combined followed by Southern blot hybridization with probes for the 18S and 28S rDNA sequences. We scored a total of 17 restriction sites, six of which were mapped conserved in all the species. The other eleven corresponded to spacer regions and revealed variations between these taxa. The study showed that the two groups differ in the length of the internal transcribed spacer. Also they showed the existence of two regions of fast evolution on the opposite termini of the external spacer. A restriction site present at low frequency in the non-transcribed spacer of guanaco and llama was the only difference encountered within the South American group.

Jorge Víctor Crisci: Latin American champion of synantherology, phenetics, cladistics and biogeography

“…The 1990s witnessed the creation in the Museo de la Plata of the Laborator y of Systematics and Evolutionar y Biology (LASBE after its Spanish acronym), which represents the formalization of an eclectic group of systematists, led by Jorge, who seek to approach taxonomic and biogeographic problems from an evolutionar y and multidisciplinar y perspective (e.g., Crisci et al, 1991a, b;Crisci et al, 1994;Morrone et al, 1994;Semorile et al, 1994;Dewey et al, 1996). During this process, Jorge directed his effor ts at another discipline, Biogeography, that had been introduced 20 years earlier by Cabrera himself.…”

Molekulargenetische Untersuchungen an Überresten präkolumbischer Neuwelt-Camelidae aus dem Palpa-Tal (Peru)

“…In anderen genetischen Abschnitten, wie z.B. dem 18sr-RNA-Gen ist kaum Diversität nachzuweisen (Semorile et al 1994). Die meisten Mikrosatelliten-Marker sind auch in Altwelt-Camelidae anwendbar (s. z.B.…”