Restriction of primary responses to the IgG class and dependency of IgM responses on secondary immunization for the copolymers of L-glutamic acid, L-tyrosine, and L-alanine.

Abstract

Primary responses to the linear polymers of L-glutamic acid, L-tyrosine, and L-alanine are restricted to the IgG class of antibodies. The appearance of specific IgM antibodies against these antigens is dependent upon secondary immunization, in contrast to many classical antigenic systems. The presence of an IgM response was verified by a direct plaque-forming cell assay, the inhibition of direct plaques by an antiserum specific for mouse micron-chain, and the physical separation of IgM and IgG GAT-specific antibodies by gel filtration. Preimmunization of the appropriate nonresponder strain with GAT or GT inhibits both the secondary IgM and IgG responses to GAT-MBSA and GT-MBSA, respectively. The tolerance observed is due to the induction of suppressor cells as demonstrated by cell transfer experiments.

Alcohol-mediated polarization of type 1 and type 2 immune responses

“…Preliminary experiments showed that BALB/c mice made very strong primary antibody responses to FγG, but rather weak responses to the DNS hapten. We previously reported (45,46) that boosting mice with antigen 3-5 d after the initial antigen priming would result in a robust antibody response. In the experiments reported here, groups of 5 BALB/c mice were fed SD, LCD, or LED30 and remained on the diets throughout the experimental protocol.…”