1967
DOI: 10.1016/0042-6822(67)90197-3
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Restriction of nonglucosylated T-even bacteriophage: Properties of permissive mutants of Escherichia coli B and K12

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Cited by 125 publications
(68 citation statements)
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“…Another example is the well-studied T4 phage, in which 5-hydroxymethylcytosine (5hmC) is incorporated into the DNA during replication and additional glucosyltransferases further modify all 5hmC to glucosylated-hydroxymethylcytosine (5ghmC). T4 genomic DNA containing 5ghmC is resistant to cleavage by most restriction endonucleases, with the exception of Type IV modification-dependent endonucleases (4,5).…”
Section: -Methylcytosine | Methylomementioning
confidence: 99%
“…Another example is the well-studied T4 phage, in which 5-hydroxymethylcytosine (5hmC) is incorporated into the DNA during replication and additional glucosyltransferases further modify all 5hmC to glucosylated-hydroxymethylcytosine (5ghmC). T4 genomic DNA containing 5ghmC is resistant to cleavage by most restriction endonucleases, with the exception of Type IV modification-dependent endonucleases (4,5).…”
Section: -Methylcytosine | Methylomementioning
confidence: 99%
“…Two independent genes control the restricting functions in E. coli K12 (10,11). The r6 function restricts all T* phages, while the r2,4 function restricts T*2 and T*4 phages, but not T*6 phage.…”
Section: Resultsmentioning
confidence: 99%
“…RR1 carries merB information from E. coli B. This mcrB function is of different specificity than the K12 activity (20,37) and may have a weaker effect. Therefore we worked in an merB::TnlO host ER1467.…”
Section: Discussionmentioning
confidence: 99%
“…It has been recently shown that certain E. coli strains have systems that block introduction of DNA containing cytosine methylation (20); the locus responsible for this activity has been designated 'mcrB' (modified gytosine restriction, 20). In RR1, this locus is derived from E. coli B and is of different specificity and apparently weaker activity (20,37). A new series of cloning strains has been constructed by inactivating this locus with TnlO insertions.…”
Section: Only Insertions Betweenmentioning
confidence: 99%