Restriction mapping of the genome of the protozoan parasite Theileria parva.

Abstract

We have used a modified linking clone strategy and pulsed-field gel electrophoresis to derive a map of the 29 Sfi I and 4 Not I sites in the 10 million base pawr genome of the protozoan parasite Theienaparva. This was achieved in the absence of classical genetic information. The map reveals four chromosomes. Several genes, including those for parasite antigens, have been located on the map, as has the single locus carrying the major repetitive sequence in this organism. The map forms the basis for a study of sexual recombination in T. parva, which will be of importance in the application of present methods ofimmunization. Aspects ofthe mapping strategy may be useful in the study of other small eukaryotic genomes.

Characterisation of single domain ATP-binding cassette protein homologues of Theileria parva

“…Hybridization of the probe to S®I-digested, agarose embedded, T. parva DNA revealed that TpABC1 was located on the second largest (1258 kb) S®I fragment (Fig. 5B), which was consistent with hybridization to chromosome 1 according to the genomic restriction map (Morzaria and Young 1992). The TpABC2 sequence was also hybridized to blots of S®I digested T. parva DNA and (HMDRN and HMDRC), the mouse MHC-linked peptide transporter, HAM1, and the Escherichia coli haemolysin exporter protein, HlyB.…”

“…It was thus of interest to determine the location of TpABC1 in the genome of T. parva. It has been shown that T. parva (Muguga) contains four chromosomes and 33 S®I fragments (Morzaria and Young 1992). Using the same conditions employed by Morzaria and Young (1992) to resolve the chromosomes of T. parva, the location of this gene was found to be on chromosome 1 of T. parva (Fig.…”

Concerted Evolution at a Multicopy Locus in the Protozoan Parasite Theileria parva: Extreme Divergence of Potential Protein-Coding Sequences†

“…The Tpr ORFs are tandemly arrayed within a single region of the T. parva genome. Previous analysis has shown that in T. parva Muguga the Tpr ORFs are present as a tandem array (3) and that the Tpr1 sequences are located on two adjacent, chromosome-internal, SfiI fragments (numbers 3 and 6) in the T. parva Muguga genome (34). In this study, a 115-bp sequence with 78% similarity to the extreme 3Ј end of the T. parva Muguga Tpr1 was identified 300 bp upstream of the large 624-and 656-codon ORFs in Uganda DNA (Fig.…”

“…The nucleotide sequence of the p67 sporozoite antigen gene (39), which is encoded on the same 780-kb SfiI fragment as the Tpr locus (34), is identical in T. parva Muguga, Boleni, and Uganda (40). The unusual conservation of the p67 gene, which extends to the 29-bp intron and the third base position of all codons, is in marked contrast to the exceptional divergence of the Tpr sequences.…”

Medium-Range Restriction Maps of Five Chromosomes ofLeishmania infantumand Localization of Size-Variable Regions

“…To define the structural basis for these Morzaria and Young, 1992; Janse et al, 1992; variations, we have constructed full-length restriction Blaineau et al, 1991; Weiden et al, 1991). In Trypamaps of five chromosomes of 370 to 490 kb in Leish-nosoma brucei and Plasmodium falciparum, karyomania infantum clone LEM1317.…”