Restriction mapping of cDNA recombinants including the adult chicken and duck globin messenger sequences: a comparative study

Reynaud, Claude‐Agnès; Tahar, S. Ben; Krust, Andrée; Franco, Maria-Pia Amaral de Lima; Goldenberg, Samuel; Gannon, Frank; Scherrer, Klaus

doi:10.1016/0378-1119(80)90066-9

Cited by 11 publications

(7 citation statements)

References 33 publications

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“…The genomic fragments were separated by electrophoresis on agarose gels, transferred onto nitrocellulose sheets (5) and hybridized with 32P labelled probes, i.e. chicken a, aA and aD globin inserts of pBR322 recombinant plasmids (6).…”

Section: Introductionmentioning

confidence: 99%

Modification of the methylation pattern in the vicinity of the chicken globin genes in avian erythroblastosis virus transformed cells

et al. 1981

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Having previously found a reduced transcription of globin genes and an abortive processing of the already transcribed globin pre-mRNA in Avian Erythroblastosis Virus (AEV) transformed cells (1), we compared the genomic DNA of these cells with that of normal chicken erythroblasts, using 32-P-labelled cDNA probes specific for the beta, alpha A and alpha D globin sequences. Restriction endonuclease digestion, electrophoresis of digests in agarose gels, Southern blotting and hybridization were carried out. Our results show that the overall genome organization is not disturbed in the immediate neighbourhood of the adult globin genes; the observed restriction fragments are identical for both DNAs after EcoRI, HindIII, BamHI and XbaI digestion, using the beta, alpha A and alpha D globin cDNA probes. However, we observe specific modifications at some methylation sites in the beta, beta-like and alpha D regions: after HpaII or MspI digestion in the alpha D region and after HhaI digestion in the beta and beta-like region, heavier bands appear in the transformed cell DNA in addition to the ones observed in normal DNA. This implies that, at some specific sites, the transformed cell DNA is more methylated than the normal erythroblast DNA. The possible significance of this observation is discussed.

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Section: Introductionmentioning

confidence: 99%

Modification of the methylation pattern in the vicinity of the chicken globin genes in avian erythroblastosis virus transformed cells

et al. 1981

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“…The chicken beta-globin probe, kindly provided by K. Sherrer and T. Imaizumi, was a TaqI restriction fragment of 620 bp of recombinant plasmid DNA carrying cDNA of chicken beta-globin (29).…”

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The chicken c-erbA proto-oncogene is preferentially expressed in erythrocytic cells during late stages of differentiation.

Hentzen¹,

Renucci²,

Guellec³

et al. 1987

Mol. Cell. Biol.

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We analyzed the expression of the c-erbA proto-oncogene in different tissues of chicken embryos. c-erbA transcripts were found at low levels in the lung, kidney, liver, and heart and in high amounts in embryonic blood cells. Nuclease mapping assays proved that these transcripts were true c-erbA transcripts. In situ hybridization on fractionated embryonic blood cells showed that c-erbA transcripts were predominantly found in erythroblasts, particularly during the final step of differentiation. Life span analysis of c-erbA mRNAs revealed their relative instability, demonstrating that the high level of c-erbA transcripts in embryonic erythroblasts was not the result of passive accumulation. These results suggest that the c-erbA genes play some role in erythrocyte differentiation.

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“…Furthermore, it is possible that there is more than one different alpha globin newly expressed in anemic chickens as determined from the sequences reported for them [I2 -141, since they are each slightly different from each of the others. At this point we must note that Scherrer and collaborators [31,32] have recently reported the construction of recombinant cDNAs containing duck globin genes. Each of their alpha globin recombinants contain PstI and EcoRI restriction endonuclease sites which our alphaglobin gene does not.…”

Section: Results a N D Discussionmentioning

confidence: 99%

Nucleotide Sequence for a Novel Duck Alpha‐Globin Gene

Paddock

Gaubatz

1981

European Journal of Biochemistry

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Nucleotide sequence analysis of a recombinant cDNA for a duck alpha‐globin gene indicates a gene with novel features and evolutionary history. The duck alpha‐globin double‐stranded cDNA, treated with S1 nuclease and tailed with poly(dC), was inserted into the PstI restriction endonuclease site of pBR322 tailed with poly(dG). Nucleotide sequence analysis of the resulting recombinant cDNA indicates that it contains the translated region and all of the 3′ untranslated region for an alpha‐globin gene. The sequence was determined by procedures designed especially for rapid analysis of pBR322 (PstI site) recombinant cDNAs. This duck globin has sequences related to the chicken alpha‐A globin in some regions but in other regions it is more closely related to another alpha globin found in anemic chickens.

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Restriction mapping of cDNA recombinants including the adult chicken and duck globin messenger sequences: a comparative study

Cited by 11 publications

References 33 publications

Modification of the methylation pattern in the vicinity of the chicken globin genes in avian erythroblastosis virus transformed cells

Modification of the methylation pattern in the vicinity of the chicken globin genes in avian erythroblastosis virus transformed cells

The chicken c-erbA proto-oncogene is preferentially expressed in erythrocytic cells during late stages of differentiation.

Nucleotide Sequence for a Novel Duck Alpha‐Globin Gene

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