Restriction fragment length polymorphisms in myogenin and myf3 genes and their influence on lean meat content in pigs

Abstract

A total of 120 pigs from farm I and 109 from farm II were examined for DdeI polymorphism in myf3 and for MspI polymorphism in myogenin genes. Meatness, weight and ratio of ham and loin meat, and loin eye area of animals with different genotypes for myf3 and for myogenin were compared. In myf3 polymorphism pigs A/A from farm I displayed significantly lower values for these characteristics. In farm II there was a reverse tendency. Present results indicate that the DdeI polymorphic region in porcine myf3 gene does not influence functionality of this gene but it may point at another polymorphism located nearby and being of functional importance. Two regions of the porcine myogenin gene were analysed for MspI polymorphism. The region encompassing coding sequences (MYOG1) was polymorphic only in three Pietrain pigs (out of 150 individuals tested) making it useless for assessment of meat productivity in pigs. The 3′ untranslated region was polymorphic and the frequency of 4.2 and 4.9 alleles varied among breeds. The 4.2 variant was predominant in Pietrain pigs. Statistical analysis indicated that meat, ham and loin meat percentages as well as loin eye area were significantly higher in 4.2/4.2 homozygotes. Further study will be undertaken to explain the relations between meat productivity and myf3 and MYOG2 alleles within breed.

Effect of myog genotypes on growth rate and production traits in Hungarian Large White pigs

“…Tendency of birth weight and growth rate data correspond to the results of Te Pas et al (1999) and Cieslak et al (2000).…”

“…Birth weight, carcass weight, growth rate and lean weight differ significantly between the MYOG genotypes (Te Pas et al, 1996, 1999Cieslak et al, 2000). The effect of the MYOG genotypes on backfat thickness has been evaluated differently (Te Pas et al, 1999;Cieslak et al, 2002).…”

Association analysis of seven candidate genes with performance traits in Czech Large White pigs

“…It can be expected that structural changes in the MYOD1 gene will have an impact on some muscle characteristics (Cieslak et al, 2000). Our results for the MYOD1 gene DdeI polymorphism (510A>C), which is in the non-coding region (intron 1), revealed no association with any analysed traits.…”

“…However, no statistically significant association with carcass and meat quality traits was found in the study of Liu et al (2008). In the study of Cieslak et al (2000) pigs from farm I with AA genotype of MYOD1 gene displayed significantly lower values for meatiness, weight and ratio of ham or loin meat and loin eye area, but the tendency was reverse in pigs from another farm. The results of the presented studies indicate that the effect of DdeI polymorphism in the MYOD1 gene may vary in different breeds or lines due to linkage disequilibrium between this SNP and the causative QTL, or due to background gene effects.…”

The analysis of carcass value in pigs of different genotypes

“…In many cases genetic researches are aimed at detection of loci (position, a part of chromosome) that have a statistically significant effect on some of the parameters of efficiency (Dvořák, 1999). The results of their research are mentioned by Kahánková et al (1996), Fiedler et al (1999), Cieslak et al (2000.In addition to relations to reproductive traits a lot of authors specialize in analyses of results of carcass value traits. Aspiration of scientific workstations is an exploitation of determined results in subsequent selection work in individual pig populations.…”