Restriction fragment length polymorphism (RFLP) of exon 2 of the <i>MhcBibi‐DRB3</i> gene in American bison (<i>Bison bison</i>)

Morris, B. G.; Spencer, Madeleine; Stabile, Samuel dos Santos; Dodd, Jonathan

doi:10.1111/j.1365-2052.1994.tb00409.x

Cited by 9 publications

(7 citation statements)

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“…1). Restriction fragment patterns (Morris et al 1994), three of which (a, f, and m) were also found in European bisui. In American bison, patterns k, q and s were not observed.…”

Section: Resultsmentioning

confidence: 81%

“…2). RFLP pattern i was shown to be present in three American bison herds (Morris et al 1994) and, in one of the herds, the frequency of the allele was estimated to be 0.540. Pattern t (111, 39, 30, and 104 bp), which was absent in cattle and present in five American herds studied by these authors, is identical to cattle pattern m (111, 69, and 104 bp) with an additional restriction site (Fig.…”

Section: Resultsmentioning

confidence: 95%

“…Digestion with Haelll produced the following RFLP patterns present in cattle and also revealed in American bison: a/a and b/b, the heterozygote a/6, the pattern corresponding to cattle c/c, which was absent in American bison (Morris et al 1994), and the heterozygote b/c (Fig. 1).…”

Section: Resultsmentioning

confidence: 95%

“…Two segment PCR was performed on thermal cycler "Techne" PHC-3 under the following conditions: 68°C -20s and 95°C -10s during 30 cycles; initial denaturation at 95°C -1 min, and final extension at 68°C -2 min. Since several copies of DRB genes with a high degree of homology may be present in a single animal (Van Eijk et al 1992, Morris et al 1994, "hot" start procedure was applied to increase the specificity of the PCR reaction (Mullis 1991). After amplification, 5-10 fil of the incubated mixture from each sample were analyzed by electrophoresis in 6% polyacrylamide gel.…”

Section: Methodsmentioning

confidence: 99%

“…In American bison Bison bison, the same procedure was used with primers specific to exon 2 BoLA-DRB3 for assessing heterogeneity between managed herds. RFLP-patterns proved to be quite scarce, and restriction sites demonstrated in cattle were found to be conserved (Morris et al 1994).…”

Section: Breeding European Bison Bison Bonasusmentioning

confidence: 98%

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Bisoniana 116. Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibo-DRB3 gene in European bison Bison bonasus

Andreassen

Bjørnbom²,

Carlsen³

et al. 1998

Acta Theriol.

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“…1). Restriction fragment patterns (Morris et al 1994), three of which (a, f, and m) were also found in European bisui. In American bison, patterns k, q and s were not observed.…”

Section: Resultsmentioning

confidence: 81%

Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Breeding European Bison Bison Bonasusmentioning

confidence: 98%

See 3 more Smart Citations

Bisoniana 116. Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibo-DRB3 gene in European bison Bison bonasus

Andreassen

Bjørnbom²,

Carlsen³

et al. 1998

Acta Theriol.

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Characterization of Bison bison major histocompatibility complex class IIa haplotypes

et al. 2005

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American bison (Bison bison) and domestic cattle (Bos taurus and Bos indicus) evolved from a common ancestor 1-1.4 million years ago. Nevertheless, they show dramatic differences in their susceptibility to infectious diseases, including malignant catarrhal fever (MCF). Although bison are highly susceptible to ovine herpesvirus-2 (OvHV-2) associated MCF, about 20% of healthy domesticated and wild bison are positive for OvHV-2 antibody. We are interested in testing the hypothesis that, within the bison population, the polymorphism of major histocompatibility complex (MHC) class II genes influences resistance to MCF. However, since little was known about the MHC class II genes of bison, it was necessary to first characterize class II haplotypes present in Bi. bison (Bibi). Thus, the MHC class II haplotypes carried by 14 bison were characterized by the PCR-based cloning and sequencing of their DRB3, DQA, and DQB alleles. Twelve MHC class II haplotypes were identified in the 14 bison. These haplotypes comprised six previously reported and six new Bibi-DRB3 alleles, along with 11 Bibi-DQA and 10 Bibi-DQB alleles. For each bison class II allele, it was possible to identify closely related cattle sequences. The closest bison and bovine DQA, DQB, and DRB3 alleles, on average, differed by only 1.3, 3.5, and 5.8 amino acids, respectively. Furthermore, bison MHC haplotypes with both nonduplicated and duplicated DQ genes were identified; these haplotypes appear to have originated from the same ancestral haplotypes as orthologous cattle haplotypes.

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Polymorphism of exon 2 of BoLA-DRB3 gene and its relationship with somatic cell score in Beijing Holstein cows

Chu

Qiao³

et al. 2011

Mol Biol Rep

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In the present study, the exon 2 and 3' end sequence of intron 1 of BoLA-DRB3 gene of 211 Beijing Holstein cows was amplified and a uniform fragment of 284 bp was obtained. The genetic polymorphism was investigated by PCR-RFLP using two restriction endonucleases HaeIII and BstYI. Seven genotypes were detected by digesting the PCR products with HaeIII. The frequency of AA, AB, AC, AD, BB, BC and BF genotypes was 0.4638, 0.0193, 0.0193, 0.3478, 0.0290, 0.0386 and 0.0822, respectively. Three genotypes were found by digesting the PCR products with BstYI. The frequency of AA, AB and BB genotypes was 0.0569, 0.2844 and 0.6587, respectively. The relationship between the polymorphisms in exon 2 of BoLA-DRB3 gene and somatic cell score (SCS) in Beijing Holstein cows was analyzed by least squares linear model. No significant difference was detected among least squares means of SCS for seven HaeIII-RFLP genotypes (P > 0.05). As for BstYI-RFLP analysis, least squares mean of SCS for AA was significantly lower than that for AB (P < 0.05) or BB (P < 0.05), and no significant difference was detected between AB and BB genotypes (P > 0.05). BstYI AA was the most favorable genotype and BstYI BB was the most unfavorable genotype for mastitis resistance. The information found in the present study is very important for improving mastitis resistance in dairy cattle by marker assisted selection.

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Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibi‐DRB3 gene in American bison (Bison bison)

Cited by 9 publications

References 4 publications

Bisoniana 116. Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibo-DRB3 gene in European bison Bison bonasus

Bisoniana 116. Restriction fragment length polymorphism (RFLP) of exon 2 of the MhcBibo-DRB3 gene in European bison Bison bonasus

Characterization of Bison bison major histocompatibility complex class IIa haplotypes

Polymorphism of exon 2 of BoLA-DRB3 gene and its relationship with somatic cell score in Beijing Holstein cows

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