Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains

Literák, Ivan; Rychlı́k, Ivan; Svobodová, Vlasta; Pospíšil, Zdeněk

doi:10.1016/s0020-7519(98)00090-3

Cited by 30 publications

(21 citation statements)

References 21 publications

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“…The great variation observed among the other isolates (D1, D2, D3, D4, D7, CH1, CH2, CH3, CH6, CH7, CH8, CH9 and CH11) has not allowed us to classify them into virulent and nonvirulent, being, thus, classified into intermediate virulent. Intermediate virulent strains are believed to be transition strains from the virulent to the non-virulent phenotype (Literák et al, 1998). Guo et al (1997) reported that intermediate virulent strains occasionally occur in nature.…”

Section: Discussionmentioning

confidence: 99%

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Brandão

Ferreira²,

Melo³

et al. 2006

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Summary :In an attempt to isolate and characterize Toxoplasma gondii from the State of Minas Gerais, Brazil, musculature samples from 72 pigs, 25 dogs, 28 free-range chickens and 50 chickens produced in industrialized farms were collected. Antibodies to T. gondii have not been detected in pigs, but were found in nine (40.9 %) out of 22 dogs, and in 15 (53.6 %) of 28 free range chickens. T. gondii was not isolated from pigs and industrialized chickens, but from eight dogs and 11 free range chickens. In order to determine T. gondii virulence, female BALB/c mice were inoculated with 10 3 , 10 2 , 10 1 and 10 0 tachyzoites of the 19 isolates. The strains RH (virulent) and ME49 (non-virulent) were used as references. Isolates were divided into three groups according to the virulence phenotype: five isolates were classified into virulent in mice, one into non-virulent and 13 into intermediate virulent. Nested-PCR of T. gondii SAG2 locus amplified DNA from 21 out of 22 DNA samples directly extracted from heart of free range chickens. These samples were genotyped through a PCR-RFLP assay. Seventeen (80.9 %) were classified into type I; one (4.8 %) into type III and three (14.3 %) into type I or II. Résumé

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Section: Discussionmentioning

confidence: 99%

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Brandão

Ferreira²,

Melo³

et al. 2006

Parasite

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“…gondii strains (Table 1) The strains used in the study included K6, K19, K24, K25 and Z92 strains isolated in the Czech Republic in 1994 and 1995 (Literák et al 1998). Strains were maintained in a tissue cyst form in mice by injections of brain homogenate every 4-6 months.…”

Section: Methodsmentioning

confidence: 99%

“…RFLP/DNA was performed as described previously (Literák et al, 1998). Isolated DNA was digested with restriction endonuclease PstI and SalI (Amersham, UK).…”

Section: Methodsmentioning

confidence: 99%

“…Probe was labelled with 32P adATP by Random Primed Labelling kit (Amersham, UK). After hybridization, membranes were washed, dried and exposed (Literák et al 1998).…”

Section: Methodsmentioning

confidence: 99%

“…Those studies documented the characteristic genotypes of virulent and avirulent strains (Cristina et al 1991ab;Sibley and Boothroyd 1992;Cristina et al 1995;Sibley 1994, 1995;Literák et al 1998). …”

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Genome Changes in the Toxoplasma gondii Strains during Laboratory Passages in Mice

Literák¹,

Rychlı́k²

1999

Acta Vet. Brno

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Literák I., I. Rychlík: Genome Changes in the Toxoplasma gondii Strains During Laboratory Passages in Mice. Acta Vet. Brno 1999, 68: 203-208. Three strains of Toxoplasma gondii isolated from rabbits (K6 and K9) and a cat (K24) in the Czech Republic in 1994-95 were maintained in a tissue cyst form in laboratory mice by injections of brain homogenate every 4 -6 months. These strains were passaged 12 to 13 times before tachyzoites, from which DNA was isolated, were obtained. PCR/RFLP (restriction fragment length polymorphism) (ROP1 gene/DdeI restriction endonuclease) and RFLP/DNA (RFLP with chromosomal DNA) (TGR1E probe/PstI and SalI restriction endonucleases) were used to characterize their genotypes. In the course of subsequent tissue cyst-tissue cyst passages, the pathogenicity of the strains increased and intraperitoneal passaging of tachyzoites from the peritoneal exudate two times weekly had to be used to facilitate the continuous maintenance. After the pathogenicity increase, the DNA was isolated from the tachyzoites after 6 to 48 tachyzoite-tachyzoite passages. Genotype differences were observed in the strain before and after increasing their pathogenicity. Both PCR/RFLP and RFLP/DNA patterns of the strain before and after increasing their pathogenicity were similar, and even identical, with previously described genotype characteristics of avirulent and virulent strains of T. gondii.

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Toxoplasma Gondii

and¹,

Dubey²

2003

Microbial Food Safety in Animal Agriculture

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Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains

Cited by 30 publications

References 21 publications

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Characterization ofToxoplasma gondiifrom domestic animals from Minas Gerais, Brazil

Genome Changes in the Toxoplasma gondii Strains during Laboratory Passages in Mice

Toxoplasma Gondii

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