Restriction fragment length polymorphism analysis of rotavirus VP7-encoding gene from humans and animals of Northeast India: a relative study of Indian and global isolates

Abstract

A restriction fragment length polymorphism (RFLP) assay was developed to examine the genetic relationship between 67 (29 Indian, 38 global) rotavirus isolates of human, bovine and porcine neonates. The assay involved direct digestion of RT-PCR amplified VP7 cDNAs with three restriction enzymes (VspI, HaeIII, NlaIV) independently. Forty-eight RFLP patterns were identified for all 67 strains, and of these 20 patterns were associated with Indian isolates. A correlation between the restriction patterns and G type was apparent through deduction of enzyme restriction sites from known sequences. Major G serotypes (G1, G2, G6, G8) with a few mixed types could be differentiated where there was a positive assortment of intrinsic serotypes from multiple host origin, and certain single or combined enzyme profiles were highly dominant in the population. Significant genetic variations were established between global and Indian isolates and none of the RFLP patterns were shared between them. These data suggest that the Indian wild-type rotavirus population is distinguishable based on the VP7 gene, and co-circulation of distinct strains in different hosts is foremost, indicating the possible likelihood of inter-species transmission.

Molecular characterization of porcine group A rotavirus to contain piglet diarrhea for productivity enhancement in North East India

“…The ELISA positive samples showed successful amplification of both VP7 and VP4 region of porcine RV by RT PCR. Notably, RVA identification from the present findings is higher in the two states in comparison to a recent report where Assam and Tripura had a positivity percentage of 23.05% and 6.54% respectively [23].…”

“…There is a wide distribution of rotavirus associated piglet diarrhea in different parts of India [2,3,21,22] with reports of higher occurrence in the north eastern states [12,14,22,23]. In the present study, out of 320 samples (120 from Assam and 200 from Tripura) screened by Monoclonal Antibody based sELISA (Premier Rota-cloneTM, Meridian Biosciences, United States) 130 samples (40.62%) were detected to be positive for RVA with an OD measure which ranged between 0.150 and 0.351.…”

“…In addition to the most prevalent RVA strains circulating worldwide, several rare and/or novel G (G1, G2-like, G6, G8, G9, G10, and G12) and P types (P [1], P [5], P [8], P [11], P [13], P [19], P14 [23], P [26]) have been detected sporadically in pigs [13]. The high incidence of diversity in the RV strains is predominantly due to accumulation of point mutations leading to genetic/antigenic drift and reassortment of cognate genes leading to genetic/ antigenic shift.…”

“…Currently, 36 G and 51 P types were described for RVA [https://rega.kuleuven.be/cev/vir almetagenomics/virus-classification/rcwg]. From global epidemiological surveys, 12 different G-types (G1 to G6, G8 to G12 and G26) and 13 different P-types (P [1], P [5] to P [8], P [11], P [13], P [19], P [23], P [26], P[27], P[32], P[34]) have been identified and reported to be associated with piglet diarrhea caused by RVA [8][9][10]. In India, 6 types of G (G3, G4, G6, G9, G10, G12) and 5 types of P (P [6], P [7], P [8], P [13], P [19]) of RVA have been detected from pigs so far (11)(12)(13).…”

Unusual rotavirus genotypes in humans and animals with acute diarrhoea in Northeast India

“…We also identified the common G1P[8] and G9P[8] genotypes in human RVA episodes along with an unusual G5P[8] genotype. Previous studies on eastern and NE Indian human RVAs detected G4, G9, G10, G12, P[4], P[6], P[7] and P[9] strains [16–18]; however, the majority of the strains were genetically distant with regional RVA isolates. Evidence of G1 RVAs in combination with a human P[8] VP4 specificity in animals was atypical with limited reports of its incidence in animal diarrhoeal cases worldwide [32–34].…”