Restriction endonuclease mapping of Co1E2-P9 and Co1E3-CA38 plasmids

Watson, Robert J.; Visentin, Louis P.

doi:10.1016/0378-1119(80)90151-1

Cited by 29 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The bacterial strain MC4100 was used as a host for the various colicinogenic plasmids: pColA9 (Lloubts et al, 1986), colicin A mutant plasmids (pDS1, pBD2, pBE1, pAR1) . ColEl-K30 (Herschman & Helinski, 1967b) ColE2-P9 and ColE3-CA38 (Watson & Visentin, 1980). The MC4100 strain containing the various plasmids was grown in LB medium (Miller, 1972).…”

Section: Methodsmentioning

confidence: 99%

Comparison of the Uptake Systems for the Entry of Various BtuB Group Colicins into Escherichia coli

Bénédetti¹,

Frenette²,

Baty³

et al. 1989

Microbiology

View full text Add to dashboard Cite

Colicins A, El, E2 and E3 belong to the BtuB group of colicins. The NH,-terminal region of colicin A is required for translocation, and defects in this region cannot be overcome by osmotic shock of sensitive cells. In addition to BtuB, colicin A requires OmpF for efficient uptake by sensitive cells. The roles of BtuB and OmpF in translocation and binding to the receptor of the colicins A, El, E2 and E3 were compared. The results suggest that for colicin A OmpF is used both as a receptor and for translocation across the outer membrane. In contrast, for colicin El, OmpF is used neither as a receptor nor for translocation. For colicins E2 and E3, the situation is intermediate: only BtuB is used as a receptor but both BtuB and OmpF are involved in the translocation step.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparison of the Uptake Systems for the Entry of Various BtuB Group Colicins into Escherichia coli

Bénédetti¹,

Frenette²,

Baty³

et al. 1989

Microbiology

View full text Add to dashboard Cite

show abstract

“…Plasmids ColE2-P9 and ColE3-CA38 carried in E. coli WA802 strain were used and purified as previously described (21). Bacteriophage M13 mp7, mp8 and mp9 replicative form DNAs (26) were purchased from PL Biochemicals.…”

Section: Materials and Methods Plasmids And Bacterial Strainsmentioning

confidence: 99%

“…Previous studies from this laboratory have localized the genes for the immunity proteins and the 3'-ends of the neighbouring colicin E2 and E3 genes to a seemingly non-homologous DNA region on the two producing plasmids, ColE2-P9 and ColE3-CA38 respectively (21)(22)(23)). In thi s report we present a comparison of the nucleotide sequences and the deduced amino acid sequences for the C-terminal peptides of colicins E2 and E3 and for their immunity proteins.…”

Section: Introductionmentioning

confidence: 99%

Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3

Lau¹,

Rowsome²,

Zuker³

et al. 1984

Nucl Acids Res

Self Cite

View full text Add to dashboard Cite

Using INTRODUCTIONThe plasmid-coded bactericidal proteins, colicins E2 and E3 and the E3-related cloacin DF13, constitute an interesting group of toxins in that each protein with an approximate Mr of 60,000 contains information for i) translocation across cell membranes, ii) receptor binding on the surface of sensitive E. coli cells and iii) nucleolytic activities (for recent reviews, see ref. 1-3). Colicin E2 is an endonuclease active on both single and double stranded DNA but with undefined specificity (4,5) whereas colicin E3

show abstract

“…L broth (Watson and Visentin 1980) was used for growth of strain HBlOl. Antibiotics were included as required at the following concentrations ( pg/mL): streptomycin, 100; Tc, 10; ampicillin (Ap), 20; Km, 20; Cm, 10; Gm, 20.…”

Section: Methodsmentioning

confidence: 99%

Derivation and molecular characterization of symbiotically deficient mutants of Rhizobium meliloti

Selvaraj¹,

Hooper²,

Shantharam³

et al. 1987

Can. J. Microbiol.

View full text Add to dashboard Cite

A collection of symbiotically defective mutants of Rhizobium meliloti JJ1c10 was derived by Tn5 mutagenesis using the suicide vector pGS9. They include two Nod− and about 250 Fix− mutants. The mutants were found to be heterogenous in acetylene reduction activity and in the morphology and ultrastructure of the nodules which they induced. Over 90% were found to contain bona fide Tn5 insertions in a variety of DNA restriction fragments. When Tn5-carrying DNA segments cloned from 24 of the mutants were introduced into the equivalent location in the genome of the wild-type strain by recombination-mediated replacement, only eight produced a symbiotically defective phenotype similar to that of the original mutant. This result indicated that many of the symbiosis mutations were not directly caused by Tn5 insertion. DNA segments apparently containing mutated fix genes but not containing Tn5 were found in eight mutants by identifying cosmids carrying wild-type DNA which complemented their symbiosis defects. Probing of the DNA of these mutants with their complementing cosmids revealed no detectable physical alteration of the homologous DNA. A segment of DNA including the hsn and nifHDK genes was favoured for these non-Tn5 mutations. Three regions of the genome in which Tn5 caused fix mutations were identified. One of these was the known megaplasmid nod-nif region. The other two regions, designated fix-e5 and fix-h21, were found to be chromosomal. Mutants in one of these chromosomal regions fluoresced more intensely on calcofluor plates than the wild type.

show abstract

Restriction endonuclease mapping of Co1E2-P9 and Co1E3-CA38 plasmids

Cited by 29 publications

References 22 publications

Comparison of the Uptake Systems for the Entry of Various BtuB Group Colicins into Escherichia coli

Comparison of the Uptake Systems for the Entry of Various BtuB Group Colicins into Escherichia coli

Comparative nucleotide sequences encoding the immunity proteins and the carboxyl-terminal peptides of colicins E2 and E3

Derivation and molecular characterization of symbiotically deficient mutants of Rhizobium meliloti

Contact Info

Product

Resources

About